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  • 1
    Electronic Resource
    Electronic Resource
    A study of the relationship between the pronephros and the haploid syndrome in frog larvaeSubmitted to the faculty of the University of Illinois in partial fulfillment of the requirements for the degree of Doctor of Philosophy. (1961)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 108 (1961), S. 203-217 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051080206
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  • 2
    Electronic Resource
    Electronic Resource
    Mechanism of repair of lenticular wounds in Rana pipiens. I. Role of cell migrationThis investigation was supported by Public Health Service Research grant 8 R01 EY 00401-02 (formerly NB 07813-02) from the National Eye Institute. (1971)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 133 (1971), S. 409-416 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The earliest visible changes that occur in the normal organization of the lens epithelium after a penetrating wound in the lens suggest that passage of an injury stimulus outward from the wound occurs within the first half day after injury: changes in normal tissue architecture appear near the wound at six hours and move outward to involve the proliferative zone by 12 hours. This is followed by migration of cells toward the wound. There is a slight increase in cell number in the proliferative zone within the first day, followed at later intervals by a decrease there and a concomitant increase in cell number adjacent to the wound. After a pre-injury injection of H3-TdR (or I125-UdR), labeled cells that had incorporated the precursor in the normal proliferative zone were found progressively closer to the wound with increasing time. Only the cells which incorporated the radioactive tracer could be followed, but it is likely that cells in the central areas also migrated toward the wound since they showed spindling and superimposition. Migration of cells into the wound margins is an important phase of wound closure which begins long before the major productions of new cells by mitosis.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051330405
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  • 3
    Electronic Resource
    Electronic Resource
    Studies on the nature and localization of acid phosphatase in normal and lens-regenerating urodele eyes. II. Biochemical assayThis investigation was supported in part by Public Health Service Research Grant 1R01-NB-07813-01 from the National Institute of Neurological Diseases, and in part by Basic Improvement Grant 1T02-CH-1017-01 from the National Institutes of Health. (1969)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 129 (1969), S. 359-367 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Biochemical assay of acid phosphatase in normal and lens-regenerating eyes of the urodele Diemictylus viridescens, using p-nitrophenyl phosphate as substrate, demonstrates both soluble and lysosomal fractions of the enzyme. While the specific activity of the soluble fraction remains unchanged during lens regeneration, the lysosomal fraction shows four distinct rises in specific activity during the thirty-day regeneration period studied. These peak activities on the second, eighth, fifteenth, and twenty-second days post-lentectomy apparently correspond to lysosomal activity in the processes of wound healing, iris depigmentation, and lens differentiation which occur during urodele lens regeneration. On the basis of biochemical and histochemical studies as well as observations of morphological changes in the urodele eye as lens regeneration proceeds, it is postulated that there is a significant correlation between these morphological changes and the level and localization of the lysosomal acid hydrolases in the tissues in which the changes occur.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051290307
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  • 4
    Electronic Resource
    Electronic Resource
    Actin filament patterns in mouse lens epithelium: A study of the effects of aging, injury, and genetics (1988)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 9 (1988), S. 17-29 
    Details
    ISSN: 0886-1544
    Keywords: sequestered actin bundles ; polygonal arrays ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Using mainly fluorescence microscopy after rhodamine-phalloidin staining, the F-actin distribution in the mouse lens epithelium was studied with regard to the effects of age, genetic strain, and mechanical injury.These studies have revealed that aside from its association with the plasma membrane the structural organization of F-actin in the mouse lens epithelium in situ is characterized by two major configurations: (1) a filamentous arrangement in such patterns as stress fibers, polygonal arrays (PAs), and meshworks, and (2) a highly concentrated structure called a sequestered actin bundle (SAB).The aging study indicated that the SAB is a consistent character in C57BL/6 mice from the age of 5 wk on, but not in CF1 mice. The size and shape of the SAB change gradually with age as inferred from two-dimensional measurements. The genetic study on the SAB character using hybrids and congenic strains showed that it is inherited as a Mendelian dominant, probably multigenic mode. Finally, the injury study revealed a structural modification in cells around the wound, including flattening of cells at the edge and extension of processes into the wound space. In the rest of the epithelium, injury amplified membrane infolding and fluorescence of polygonal arrays but diminished the size and fluorescence intensity of SABs. These changes are thought to be correlated with wound repair involving cell division and migration.These studies illustrate the variability in F-actin expression in situ in lens epithelial cells that can be induced by intrinsic and extrinsic factors.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970090104
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  • 5
    Electronic Resource
    Electronic Resource
    Near-UV radiation disrupts filamentous actin in lens epithelial cells (1993)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 26 (1993), S. 40-48 
    Details
    ISSN: 0886-1544
    Keywords: actin ; tubulin ; vimentin cytoskeleton ; cataract ; elasmobranch lens ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ultraviolet radiation in the near range (UVA) causes lens opacification and disrupts the actin cytoskeleton in rabbit and gray squirrel lenses. Changes were noted using transmission electron microscopy of tangential sections and rhodaminephalloidin fluorescence microscopy of epithelial whole mounts of irradiated and unirradiated lenses, and corresponded with gross cataract formation. Irradiated lenses lacked microfilament polygonal arrays at the inner surface of the apical plasma membrane (i.e., in the cell pole next to the lens fibers) in lens epithelia of both species; a condensed actin bundle was present instead. This bundle, and scattered small actin clumps in the cytoplasm, were identified by immunogold TEM, using a specific antibody and a secondary antibody conjugated with coloidal gold. Similar techniques showed breakdown of tubulin and vimentin, but after longer intervals than for the breakdown of actin. Generalized cytologic damage was also present in epithelial cells, but not in the underlying cortical lens fibers. Damage began to occur after 4 hr of irradiation and became more severe with increased exposure. Shielded controls remained clear, had normal cytology and polygonal arrays, and no clumping of actin filaments. © 1993 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970260105
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  • 6
    Electronic Resource
    Electronic Resource
    Studies of an injury-induced growth in the frog lensThis investigation was carried out during the tenure of a traineeship (Graduate Training Grant in Anatomy no. 2G-286(C2)) from the National Institutes of Health, U. S. Public Health Service. (1963)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 146 (1963), S. 299-311 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: A tumor-like growth in the lens of Rana pipiens occurs following mechanical injury to the lens. The frequency of occurrence and size of the growth are roughly dependent on the extent of the injury inflicted. The growth first appears four days after wounding, grows actively for three weeks and usually begins to regress after a month. The cells of the growth appear to originate by proliferation of the lens epithelialcells surrounding the wound which grow into and actively invade the lens fibers. The lens growth was not transplantable either homologously into the anterior eye chamber after dissociation, nor isologously in a subcutaneous site. Protein, LDH, MDH, and G-6-PD patterns obtained after electrophoretic separation in starch gels were similar for normal and the abnormal lenses. Whether a true tumor or hyperplasia, the froglens exhibits an unique reaction to the injury stimulus which is not found in mammals (man, rabbit, mouse). The controversial tumor immunity of the mammalian lens appears to the related to an innate resistance of the epithelium to proliferation which is illustrated in part by the species difference in the reactivity of the mammalian lensand the amphibian lens to injury.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091460404
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  • 7
    Electronic Resource
    Electronic Resource
    Propagation and prolongation of mitotic activity in the formation of injury-induced lentomas in Rana pipiensThis investigation was supported in part by Public Health Service Research grant NB-056 35-01 from the National Institute of Neurological Diseases and Blindness, and in part by an Institutional Research Grant from The Johns Hopkins University (allocation from NIH General Research Support grant 1-S01-FR-005378-01). (1965)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 153 (1965), S. 111-127 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Autoradiography following labeling with H-3-thymidine was used to study the pattern of growth of injury-induced lentomas in Rana pipiens. This reaction to injury was compared with the more controlled reaction in the rabbit lens, which has been extensively studied by others.These studies show that, unlike that of the rabbit, the entire lens epithelium of the frog reacts within a day to the injury stimulus by disarrangement of its normal architecture. H-3-thymidine is incorporated between 24 and 40 hours post-injury, followed subsequently by widespread mitotic activity. Peak thymidine incorporation occurs at three days and peak mitosis at five days post-injury. However, the stimulus to mitotic activity is propagated after the first week largely among the superficial cells of the enlarging lentoma and adjacent normal epithelium; DNA synthesis and mitosis in the deeper cells of the lentoma diminish at a week and cease by two weeks post-injury, and by three weeks in the superficial cells.It is concluded that the frog lentoma is formed by accretion from the surface as a result of extensive multiplication of cells throughout the injured epithelium. The reaction of frog and rabbit lenses to injury differs both in the extensiveness of the initial reaction and the duration of the stimulus to proliferation.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091530202
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of cell populations of normal and injured mouse lens epithelium. I. Cell cycleThis work was supported by Public Health Service Research Grant 5 R01-EY 00698-03, -04, -05 from the National Eye Institute. (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 186 (1976), S. 105-113 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Using a combination of single isotope and double isotope autoradiography after injection of 3H-thymidine and of 3H- + 14C-thymidine, respectively, the cell cycle of normal and injured lens epithelial cells in the mouse was determined. Progenitor cells in the peripheral region of normal lens epithelium were found to traverse the replicative cycle in the same amount of time as the injury-stimulated cells in both peripheral and central (wound) regions. The individual phases were also the same length, except for a slight shortening of G2 in the injured epithelium. The durations of the phases were: S: 11-12 hours; G2: 1.5-2 hours; M: 4.2-5 hours; G1 (derived): 38-44 hours; and total cycle: 55-61 hours. Two findings were significant in view of previous observations that injury to mouse lens seldom induces lens opacity. First, while the cell cycle duration was not affected by injury, a burst of proliferation of potentially active cells ensued. And, secondly, this burst of proliferation involved only one cell cycle. Only a small number of cells, equivalent to the normal progenitor compartment, continued into a second replicative cycle. The wound was healed, therefore, mainly by one division cycle involving a large number of cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091860108
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  • 9
    Electronic Resource
    Electronic Resource
    The cytoarchitecture of normal mouse lens epitheliumThis investigation was supported by Public Health Service Research grant 7 R01 EY00698-01 from the National Eye Institute. (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 173 (1972), S. 225-228 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The lens epithelium of 30 gm male albino CF1 mice was characterized by determining the area dimensions and the mitotic rate of the total and of the several different regions of whole-mount preparations. A ring of nonpigmented iris cells is found to adhere to the outer surface of the lens cuticle, which serves to delineate an inner central zone from an outer peripheral zone of the epithelium. A high number of dividing cells in the peripheral area, especially immediately adjacent to the meridional rows, but including the area overlain by the iridial fringe of cells, identifies this wide region as the proliferative zone. The mitotic rate, furthermore, undergoes marked diurnal variation, rising in the late evening through the early morning hours and diminishing during the late morning and afternoon hours.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091730212
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of cell populations of normal and injured mouse lens epithelium. II. Contribution of local and migrating cells to wound healingThis work was supported by Public Health Service Research Grant 5 R01-EY 00698-03, -04, -05 from the National Eye Institute. (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 186 (1976), S. 115-120 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The contribution of progenitor cells in the normal peripheral region of mouse lens epithelium to healing of a centrally located wound was studied using an autoradiographic technique designed to follow migrating 3H-thymidine labeled cells. It was found that few progenitor cells labeled before or at the time of injury migrate toward the wound and hence contribute little to the repair of the wound. Moreover, the central injury did not delay or reverse the normal migration of the labeled peripheral cells in the opposite direction into the bow. It is concluded from this and other studies that a central injury to the mouse lens is healed mainly by numerous local cells which are stimulated for the most part to traverse one replicative cycle of normal duration, and that the injury does not affect formation of new lens fibers. These findings are thought to be related to the observed low incidence of traumatic cataract in the mouse.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1091860109
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