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Selenium protects primary human keratinocytes from apoptosis induced by exposure to ultraviolet radiation (2003)

Rafferty, T. S. ; Beckett, G. J. ; Walker, C. ; [et al.]

Oxford, UK : Blackwell Science Ltd

Clinical and experimental dermatology 28 (2003), S. 0

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ISSN: 1365-2230

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Summary The generation of reactive oxygen species has been implicated in ultraviolet radiation (UVR)-induced skin damage. In mice, increasing dietary selenium intake protects skin from UVR-induced DNA damage and photocarcinogenesis. We sought to determine whether selenium supplementation could protect keratinocytes from apoptosis resulting from exposure to broadband (TL20W/12) UVR. Unirradiated cultures contained 6.5 ± 1% apoptotic cells; the maximum percentage of apoptotic cells (34 ± 5%) was seen 16 h after UVR of 600 J/m2. Under these conditions cell death from necrosis was 15 ± 2.5% of the total cells. A 24-h preincubation with sodium selenite (10 nm−1 µm) or selenomethionine (50 nm−1 µm) protected cultured human keratinocytes from UVR-induced apoptosis. In primary keratinocytes the greatest reduction in apoptosis was found with 100 nm of either selenium compound (71% reduction in the numbers of total apoptotic cells; P 〈 0.01). Supplementation with 100–200 nm selenite or selenomethionine prevented UVR-induced apoptosis, but did not decrease the levels of UVR-induced p53, as measured by Western blotting. Collectively, this data suggests that selenium prevents UVR-induced cell death by inhibiting p53-independent cell death pathways.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1365-2230.2003.01254.x

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A clinical computer database entry form for an intraoperative anesthesiology-based transesophageal echocardiography monitoring service (1993)

Rafferty, T. ; Davis, E. ; Lippmann, H. ; [et al.]

Springer

Journal of clinical monitoring and computing 10 (1993), S. 235-245

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ISSN: 1573-2614

Keywords: database ; entry form ; transesophageal ; echocardiography ; intraoperative

Source: Springer Online Journal Archives 1860-2000

Topics: Computer Science , Medicine

Notes: Abstract Transesophageal echocardiography (TEE) has been increasingly applied to supplement and, in instances, to supplant conventional intraoperative cardiac monitoring. Our body of experience (〉1600 intraoperative TEE procedures), combined with insights gleaned from an intramural quality assurance study, and clinical implications of certain recent advances in the field, led us to develop the following TEE computer database entry form. The form, completed intraoperatively, consists of a patient and surgical procedure demographics section, followed by fields based on the TEE examination. The scans encompass transverse plane basal short axis, long axis, and transgastric views of the heart and great vessels. The two-dimensional echocardiographic, saline-contrast, color flow and pulsed Doppler data represent both right and left ventricular performance, valvular function and specific lesions. This database entry form is intended to serve as a guide for performance of a nominally complete intraoperative study and facilitate maintenance of a TEE archive consistent with current advances.