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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 793 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Industrial and engineering chemistry 14 (1975), S. 250-256 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Molecular biology reports 10 (1984), S. 91-97 
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA binding proteins present in the cytoplasm and nuclei of term placenta were isolated by DNA-cellulose chromatography and analysed by electrophoresis in high resolution polyacrylamide gradient gels. A denatured DNA specific protein of approximate molecular weight 34 000 daltons was the predominant DNA binding protein of the cytoplasm; this protein consisted of over 65% of the total DNA binding proteins of the 0.15 M NaCl eluate of the cytoplasm. The cytoplasmic extracts contained two additional DNA binding proteins of molecular weight 24 000 and 18 000 daltons and these proteins bound preferentially to ds DNA. All the three DNA binding proteins were also present in the nuclei and electrophoresis of histones in adjacent lanes indicated that they are not histones. The 34 000-dalton DNA binding protein has been purified by ammonium sulphate fractionation followed by phosphocellulose (PC) chromatography. The DBP eluted from the PC column between 0.125–0.15M potassium phosphate. PC fractions containing electrophoretically pure 34KD DBP showed an endonuclease activity capable of converting plasmid pBR 322 DNA to the linear form. Maximum endonucleolytic activity was observed in the presence of 3–5 mM Mg2+ and the enzyme activity was completely inhibited by 3 mM ethylenediamine tetraacetate.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    The international journal of angiology 5 (1996), S. 65-69 
    ISSN: 1615-5939
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study was to compare the short-term and long-term benefits and complications of patients subjected to aortic balloon valvuloplasty with those of a similar group of patients subjected to aortic valve replacement. Both groups were matched for age and sex and followed up to December 1991. The study period for the valvuloplasty group was from 1986 to 1991. The surgical group was studied from 1979 to 1991. Clinical and hemodynamic data were collected prospectively. Short- and long-term follow-ups were collected retrospectively from chart reviews, telephone inquiries with patients, immediate relatives, and family physicians. A total of 66 patients were studied; 33 had balloon valvuloplasty. A similar number of surgical patients were randomly selected from a group of 60 who had aortic valve replacement, to match the age and sex of the valvuloplasty group. Baseline, clinical, and hemodynamic characteristics were similar in both groups. All patients had right, retrograde, and transseptal left heart catheterization. Gradient across aortic valve was measured by simultaneous recording of pressures in left ventricle and aorta. Indicator dilution curves were used to calculate cardiac index and assess severity of aortic regurgitation. Patients with regurgitant fraction 〉25% were excluded from aortic valvuloplasty. Although inhospital mortality was higher in surgically treated patients (12% vs 9%) the 5-year survival of valve replacement was much better than those treated with balloon valvuloplasty (71% vs 7%). Our selection of patients and immediate results of valvuloplasty are comparable to seven published series. Aortic balloon valvuloplasty should not be used as a routine or elective procedure for treatment of adult patients with aortic stenosis. It could, however, be considered as a “bridge” procedure to stabilize high-risk patients before surgical replacement of the valve.
    Type of Medium: Electronic Resource
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