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  • 1
    Electronic Resource
    Electronic Resource
    Potentiation of delayed-type hypersensitivity response to syngeneic tumors in mice prevaccinated with cells modified by hydrostatic pressure and crosslinking (1991)
    Springer
    Cancer immunology immunotherapy 33 (1991), S. 1-8 
    Details
    ISSN: 1432-0851
    Keywords: Tumor antigens ; Hydrostatic pressure ; Crosslinking ; DTH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Delayed-type hypersensitivity (DTH) to the chemically induced EL4 and virally induced ARadLV 136 leukemia cells was determined by the radioactive ear test. Prior to the DTH test, mice were prevaccinated with cells treated either with hydrostatic pressure or with the membrane-impermeant crosslinker adenosine dialdehyde or with a combination of these. For both tumor cells, DTH against unmodified cells was markedly potentiated by prevaccination with cells treated with hydrostatic pressure combined with adenosine dialdehyde. In vitro cytotoxicity data indicated that maximal lysis of target cells is induced by vaccination with adenosine-dialdehyde-treated cells, as well as by pressure in combination with adenosine dialdehyde treatment. In addition, increased [3H]thymidine uptake was observed in effector T cells induced by prevaccination with tumor cells modified by adenosine dialdehyde or pressure or both. This novel and innocuous potentiation of an antitumor immune response may have a practical utility in the treatment of human cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01742520
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  • 2
    Electronic Resource
    Electronic Resource
    Increased projection of MHC and tumor antigens in murine B16-BL6 melanoma induced by hydrostatic pressure and chemical crosslinking (1993)
    Springer
    Cancer immunology immunotherapy 36 (1993), S. 293-299 
    Details
    ISSN: 1432-0851
    Keywords: Hydrostatic pressure ; Crosslinking ; MHC ; Tumor antigen ; B16-BL6 melanoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The B16-BL6 melanoma, like most spontaneously arising tumors, is poorly immunogenic and expresses low levels of major histocompatibility complex (MHC) antigens. Treatment of cells of this tumor in vitro by hydrostatic pressure in the presence of adenosine 2′,3′-dialdehyde (oxAdo), a membrane-impermeant crosslinker, caused elevated projection of MHC and a specific tumor antigen as demonstrated by flow-cytometric analysis. Maximum projection of both the MHC and the tumor antigens could be reached by application of 1200 atm for 15 min in the presence of 20 mM oxAdo. It is not yet clear whether this passive increase in availability of antigens on the cell surface originated from a dormant pool of antigens in the plasma membrane or from pressure-induced fusion of antigen-rich intracellular organelles (e.g. the endoplasmic reticulum). The immunogenic properties of the antigen-enriched B16-BL6 cells are described in the following paper.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01741167
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of cell-mediated immunity against B16-BL6 melanoma in mice vaccinated with cells modified by hydrostatic pressure and chemical crosslinking (1993)
    Springer
    Cancer immunology immunotherapy 36 (1993), S. 300-306 
    Details
    ISSN: 1432-0851
    Keywords: Hydrostatic pressure ; Crosslinking ; B16-BL6 melanoma ; Tumor vaccine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In the preceding paper we have demonstrated an increase in presentation of both major histocompatibility complex antigens (MHC) and a tumor-associated antigen of the weakly immunogenic B16 melanoma by a straight-forward technique. The method consists in modulating the tumor cell membrane by hydrostatic pressure and simultaneous chemical crosslinking of the cell-surface proteins. In B16-BL6 melanoma, the induced antigenic modulation was found to persist for over 48 h, which permitted the evaluation of the ability of modified B16-BL6 cells to induce immunity against unmodified B16-BL6 cells. In the present study, we have shown that a significant systemic immunity was induced only in mice that were immunized with modified B16-BL6 melanoma cells, whereas immunization with unmodified B16-BL6 cells had only a marginal effect when compared to the results in control sham-immunized mice. The induced immunity was specific since a single immunization affected the growth of B16-BL6 tumors but had no effect on MCA 106, an antigenically unrelated tumor. The addition of interleukin-2 to the immunization regimen had no effect on the antitumor responses induced by the modified B16-BL6 cells. The cell-mediated immunity conferred by immunization with treated B16-BL6 cells was confirmed in experiments in vitro where splenocytes from immunized mice could be sensitized to proliferate by the presence of B16-BL6 cells. In addition, the altered antigenicity of these melanoma cells appeared to correlate with their increased susceptibility to specific effectors. Thus,51Cr-labeled B16-BL6 target cells, modified by pressure and crosslinking, in comparison to control labeled target cells, were lysed in much greater numbers by effectors such as lymphokine-activated killer cells and allogeneic cytotoxic lymphocytes (anti-H-2b), while such cells remained resistant to lysis by natural killer cells. Our findings indicate that the physical and chemical modifications of the tumor cells that are described here may be considered as a simple yet effective method for the preparation of tumor vaccines, which could be applied in tumor-bearing hosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01741168
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Approaches to implement bispecific antibody treatment of ovarian carcinoma (1997)
    Springer
    Cancer immunology immunotherapy 45 (1997), S. 187-189 
    Details
    ISSN: 1432-0851
    Keywords: Key words T cell retargeting ; Ovarian carcinoma ; CD28 costimulation ; Human antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002620050429
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of three different methods for radiolabelling human activated T lymphocytes (1997)
    Springer
    European journal of nuclear medicine 24 (1997), S. 497-504 
    Details
    ISSN: 1619-7089
    Keywords: Lymphocyte radiolabelling ; Bispecific monoclonal antibody ; Ovarian cancer ; Adoptive immunotherapy ; Indium-111 oxine ; Technetium hexamethylpropylene amine oxime ; Fluorine-18 2-fluoru-2-deoxy-d-glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One approach in the treatment of ovarian cancer MOv18/anti-CD3 (biMAb OC/TR), which recognizes a 38-kDa glycoprotein expressed on ovarian carcinomas and the CD3 T cell receptor. However, little is known about the in vivo biodistribution of injected activated lymphocytes, information that could be obtained by scintigraphic imaging of radiolabelled T cells in order to visualize the migratory pattern. We compared the efficiency, stability and toxicity of technetium-99m hexamethylpropylene amine oxime (HMPAO),indium-111 oxine and fluorine-18 2-fluoro-2-deoxy-d-glucose (FDG) in radiolabelling activated lymphocytes targeted with biMAb OC/TR. The mean labelling efficiencies of111In-oxine and18F-FDG using 2.5×108 lymphocytes (68% and 64%, respectively) were more than twice that of99mTc-HMPAO (31%). Retention of the radionuclide in the cell was highest in the case of111In-oxine labelling (less than 25% of the initial cell-bound activity released after 240 min, as compared with 44% of the99mTc label in the same period and 45% of18F radionuclide released after 150 min). None of the three radiolabelling reagents induced any significant alteration in cell viability or immunophenotype. However, both111In-oxine and18F-FDG induced a loss of cytotoxic activity of lymphocytes against the ovarian carcinoma cell line IGROV1, and all three radiolabelling reagents caused a significant reduction in the proliferative ability of labelled lymphocytes compared to controls, with cell death occurring after 8–9 days. Radiolabelling with the more stable111In-oxine reagent using a higher number of lymphocytes (1.4x109) but the same total activity (around 55.5 MBq) resulted in improved labelled T cell viability and proliferative ability, although the mean labelling efficiency decreased (35.8%). Together the data suggest that111In-oxine at low activity per cell is the most appropriate reagent for radiolabelling activated retargeted T lymphocytes useful for in vivo biodistribution studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01267680
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison of three different methods for radiolabelling human activated T lymphocytes (1997)
    Springer
    European journal of nuclear medicine 24 (1997), S. 497-504 
    Details
    ISSN: 1619-7089
    Keywords: Key words: Lymphocyte radiolabelling ; Bispecific monoclonal antibody ; Ovarian cancer ; Adoptive immunotherapy ; Indium-111 oxine ; Technetium hexamethylpropylene amine oxime ; Fluorine-18 2-fluoro-2-deoxy-d-glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. One approach in the treatment of ovarian cancer patients involves the infusion of autologous T lymphocytes coupled with a bispecific monoclonal antibody MOv18/anti-CD3 (biMAb OC/TR), which recognizes a 38-kDa glycoprotein expressed on ovarian carcinomas and the CD3 T cell receptor. However, little is known about the in vivo biodistribution of injected activated lymphocytes, information that could be obtained by scintigraphic imaging of radiolabelled T cells in order to visualize the migratory pattern. We compared the efficiency, stability and toxicity of technetium-99m hexamethylpropylene amine oxime (HMPAO), indium-111 oxine and fluorine-18 2-fluoro-2-deoxy-d-glucose (FDG) in radiolabelling activated lymphocytes targeted with biMAb OC/TR. The mean labelling efficiencies of 111In-oxine and 18F-FDG using 2.5×108 lymphocytes (68% and 64%, respectively) were more than twice that of 99mTc-HMPAO (31%). Retention of the radionuclide in the cell was highest in the case of 111In-oxine labelling (less than 25% of the initial cell-bound activity released after 240 min, as compared with 44% of the 99mTc label in the same period and 45% of 18F radionuclide released after 150 min). None of the three radiolabelling reagents induced any significant alteration in cell viability or immunophenotype. However, both 111In-oxine and 18F-FDG induced a loss of cytotoxic activity of lymphocytes against the ovarian carcinoma cell line IGROV1, and all three radiolabelling reagents caused a significant reduction in the proliferative ability of labelled lymphocytes compared to controls, with cell death occurring after 8–9 days. Radiolabelling with the more stable 111In-oxine reagent using a higher number of lymphocytes (1.4×109) but the same total activity (around 55.5 MBq) resulted in improved labelled T cell viability and proliferative ability, although the mean labelling efficiency decreased (35.8%). Together the data suggest that 111In-oxine at low activity per cell is the most appropriate reagent for radiolabelling activated retargeted T lymphocytes useful for in vivo biodistribution studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01267680
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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