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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    BioMetals 5 (1992), S. 73-80 
    ISSN: 1572-8773
    Keywords: mercury ; arsenic ; cadmium ; plasmid ; restriction analysis ; curing ; conjugation ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pseudomonas sp isolated from the Bay of Bengal (Madras coast) contained a single large plasmid (pMR1) of 146 kb. Plasmid curing was not successful with mitomycin C, sodium dodecyl sulfate, acridine orange, nalidixic acid or heat. Transfer of mercury resistance from marinePseudomonas toEscherichia coli occurred during mixed culture incubation in liquid broth at 10−4 to 10−5 ml−1. However, transconjugants lacked the plasmid pMR1 and lost their ability to resist mercury. Transformation of pMR1 intoE. coli competent cells was successful; however, the efficiency of transformation (1.49×102 Hgr transformants μg−1 pMR1 DNA) was low.E. coli transformants containing the plasmid pMR1 conferred inducible resistance to mercury, arsenic and cadmium compounds similar to the parental strain, but with increased expression. The mercury resistant transformants exhibited mercury volatilization activity. A correlation existed between metal and antibiotic resistance in the plasmid pMR1.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Vesicular-arbuscular mycorrhizae ; Occurrence ; Coal waste ; Lignite ; Calcite ; Mine spoils ; Revegetation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We assessed vesicular-arbuscular mycorrhizal (VAM) fungi in coal, lignite, and calcite mine spoils. The level of VAM fungal infection and the population of VAM species in plants on the coal-waste sites were similar to those in plants on the calcite mine spoil. The plants on the coal-waste sites and their associated VAM fungi included Tephrosia purpurea (L.) Pers. with Glomus heterosporum and Sclerocystis microcarpus and Euphorbia hirta L. with G. botryoides and G. ambisporum. The recently revegetated lignite mine spoil supported Eragrostis sp. with G. globiferum, while the oldest revegetated lignite mine spoil supported Cocos nucifera L. with Scutellospora aurigloba and G. aggregatum, and Albizia lebbeck (L.) Benth. with Scutellospora dipapillosa and G. tenue. In the calcite mine spoil, roots of Mukia maderaspatana (L.) M. Romer with Sclerocystis sinuosa, G. dimorphicum, G. heterosporum and G. ambisporum and Datura metal L. with G. pustulatum and G. pulvinatum were found. The level of VAM infection and the population of VAM species in the oldest revegetated site (lignite mine spoil) were comparable with those in the coal-waste and calcite mine-spoil sites.
    Type of Medium: Electronic Resource
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