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  • 1
    ISSN: 1573-1561
    Keywords: Brassica ; Pratylenchus neglectus ; nematode ; biological control ; suppression ; glucosinolate ; isothiocyanate ; aliphatic ; 2-phenylethyl ; nematicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The potential of tissue amendments from a variety of wild and cultivated Brassica spp. to kill the root lesion nematode (Pratylenchus neglectus) in soil was assessed in laboratory experiments. Soil amended with leaf tissues was highly nematicidal, killing 56.2–95.2% of exposed nematodes. Amendment with root tissues was less effective, causing 0–48.3% mortality. Little of the suppressive impact by leaf tissues could be related to either total contents or any individual glucosinolate as determined by HPLC analysis. While the levels of total glucosinolates within root amendments had a nonsignificant relationship to nematode mortality, levels of 2-phenylethyl glucosinolate within root amendments significantly correlated with nematode suppression (P 〈 0.001). Amendment of soils with equimolar levels of purified 2-phenylethyl isothiocyanate resulted in comparable levels of nematode mortality, suggesting that 2-phenylethyl glucosinolate has a role in the suppressive impact of Brassica spp. root tissues.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Brassica napus ; Pratylenchus neglectus ; nematode ; 2-phenylethyl glucosinolate ; isothiocyanate ; pest resistance ; disease break ; variability ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Root concentrations of 2-phenylethyl glucosinolate in canola, Brassica napus, influence the susceptibility of the crop to the root lesion nematode (Pratylenchus neglectus), as well as the nematicidal effect of root tissues as they degrade in the soil. Plants containing high 2-phenylethyl glucosinolate should therefore reduce soil populations of P. neglectus. A selection program was developed to increase the proportion of total glucosinolates contributed by 2-phenylethyl glucosinolate in the roots of B. napus cv. Dunkeld. Variation within this accession was observed to be stable across the S1 and S2 generations. The segregation observed for 2-phenylethyl glucosinolate percentage suggested that the trait was encoded at a single locus, with the "high" phenotype being dominant. Plants with the high 2-phenylethyl glucosinolate phenotype (〉45% of total glucosinolates) were shown to be significantly more resistant to P. neglectus than otherwise identical "low" phenotypes (〈45% of total).
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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