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  • 1
    Electronic Resource
    Electronic Resource
    Lipopolysaccharide of Rhodospirillum salinarum 40: structural studies on the core and lipid A region (1995)
    Springer
    Archives of microbiology 164 (1995), S. 280-289 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsRhodospirillum salinarum ; Lipopolysaccharide ; Mixed lipid A ; 2 ; 3-Diamino-2 ; 3-dideoxy-d-glucose ; 4-O-Methyl- ; galacturonic acid ; Halophilic bacteria ; Lethal toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The structural elucidation of lipid A of the cell wall lipopolysaccharide (LPS) of Rhodospirillum salinarum 40 by chemical methods and laser desorption mass spectrometry revealed the presence of a mixed lipid A composed of three different 1,4′bisphosphorylated β(1→6)-linked backbone hexosaminyl-hexosamine disaccharides, i.e. those composed of GlcN→GlcN, 2,3-diamino-2,3-dideoxy-d-Glc-(DAG)→DAG, and DAG→GlcN. Lipid A of R. salinarum contained preferentially 3-OH-18 : 0 and 3-OH-14 : 0 as amide-linked and cisΔ11–18 : 1 and c19 : 0 as ester-linked fatty acids. The mass spectra of the liberated acyl-oxyacyl residues proved the concomitant presence of 3-O-(cisΔ11–18 : 1)–18 : 0 and 3-O-(c19 : 0)-14 : 0 as the predominating diesters in this mixed lipid A. The glycosidically linked and the ester-linked phosphate groups of the backbone disaccharide were neither substituted by ethanolamine, phosphorylethanolamine, nor by 4-amino-4-deoxy-l-arabinose, in contrast to most of the enterobacterial lipid As. In the core oligosaccharide fraction, a HexA (1→4)HexA(1→5)Kdo-trisaccharide was identified by methylation analysis. The terminal HexA (hexuronic acid) is possibly 4-OMe-GalA, a component described here as an LPS constituent for the first time. LPS of R. salinarum showed a lethality in C57BL/10 ScSN (LPS-responder)-mice) of an order of 10–1–10–2 of that reported for Salmonella abortus equi LPS, and it was also capable of inducing TNFα and IL6 in macrophages of C57BL/10ScSN mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050265
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and chemical characterization of lipopolysaccharides from four Mycoplana species (M. bullata, M. segnis, M. ramosa and M. dimorpha) (1993)
    Springer
    Archives of microbiology 159 (1993), S. 445-452 
    Details
    ISSN: 1432-072X
    Keywords: Mycoplana ; LPS ; 2,3-Diamino-glucose ; 4-Oxo-14:0 ; DOC-PAGE ; Phylogeny ; Pseudomonas vesicularis ; Agrobacterium tumefaciens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lipopolysaccharides (LPS), isolated from four Mycoplana species, i.e. the type strains of M. bullata, M. segnis, M. ramosa and M. dimorpha, were characterized onto their chemical composition and their respective lipid A-types. Those of M. bullata and M. segnis showed on DOC-PAGE an R-type character and had lipid A's of the Lipid ADAG-type which exclusively contained 2,3-diamino-2,3-dideoxy-d-glucose as lipid A sugar. LPS's of M. ramosa and M. dimorpha showed, although only weakly expressed, ladder-like patterns on DOC-PAGE indicating some S-type LPS's and lipid A of the d-glucosamine type (Lipid AGlcN). M. bullata LPS contained mannose and glucose in major amounts and additionally l-glycero-d-mannoheptose, whereas M. segnis LPS was composed of rhamnose, mannose and glucose together with both, d-glycero-d-manno- and l-glycero-d-manno-heptoses in a molar ratio of 1:2. All LPS's contained 2-keto-3-deoxy-octonic acid (Kdo), phosphate and an unidentified acidic component “X”. In addition to “X”, M. segnis LPS contained glucuronic and galacturonic acids, whereas M. ramosa LPS contained only galacturonic acid. Acetic acid hydrolysis of the LPS resulted in splitting off lipid A moieties, very rich in 3-hydroxy fatty acids, in particular in 3-OH-12:0 (in Lipid ADAG), or in 3-OH-14:0 (in Lipid AGlcN). Analysis of the 3-acyloxyacyl residues revealed major amounts of amide-linked 3-OH(3-OH-13:0)12:0 in lipid A of M. bullata and 3-OH(12:0)12:0 in lipid A of M. segnis. The rare 4-oxo-myristic acid (4-oxo-14:0) was observed only in M. bullata LPS, where it is ester-linked. Amide linked diesters could not be traced in M. ramosa and M. dimorpha. All four lipid A's lacked erster-bound acyloxyacyl residues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288592
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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