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  • 1
    Electronic Resource
    Electronic Resource
    Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 40 (1991), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1991.tb02412.x
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  • 2
    Electronic Resource
    Electronic Resource
    Dynamics of Polymyxa graminis and Indian peanut clump virus (IPCV) infection on various monocotyledonous crops and groundnut during the rainy season (2002)
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 51 (2002), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The progress of Indian peanut clump virus (Hyderabad isolate; IPCV-H) and its vector Polymyxa graminis in various monocotyledonous crops and groundnut was studied during the 1994, 1995 and 1996 rainy seasons in a naturally infested field in India. The roles of rainfall and temperature in the dynamics of infection by both the virus and its vector were analysed by exposing young seedlings for short periods in the field. Of the host crops studied, wheat, followed by barley, showed the highest virus incidence, although P. graminis was rarely observed in roots of wheat and was not detected in those of barley. The roots of maize, pearl millet and sorghum plants infected by P. graminis showed intense colonization by sporosori. IPCV accumulated in systemically infected maize plants; the sorghum and pearl millet cultivars studied showed a transient presence of IPCV-H. Rice was seldom infected by the virus and P. graminis was not detected in its roots. Groundnut was a systemic host for the virus, although during these experiments no P. graminis was found in its roots. Groundnut appeared to be susceptible to infection, mostly in the early stages of crop development, and the rate of IPCV-H transmission in groundnut seeds was highest (13%) for plants infected when young. The seed-transmission rate quickly decreased in plants showing symptoms 1 month after sowing. Time of infection had little influence on groundnut pod yield, which was always reduced by 〉60% in infected plants. There was some evidence that the quantity and distribution of rainfall influenced the incidences of IPCV-H and P. graminis: high rainfall resulted in high incidences of the virus and P. graminis, and a weekly rainfall of 14 mm was sufficient for P. graminis to initiate infection. Temperatures prevailing during the rainy season ranged from 23 to 30°C and were found to be conducive to natural virus transmission. These results suggest measures to be explored for controlling peanut clump disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3059.2002.00752.x
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a potyvirus associated with bushy dwarf symptom in chickpea, Cicer arietinum, in India (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A potyvirus that induced stunting and a characteristic bushy appearance at the apical region, due to proliferation of terminal branches with narrowed, reduced and deformed leaflets, was isolated from chickpea in India. The virus was sap-transmissible to 14 species of Chenopodiaceae, Leguminosae, Solanaceae and Malvaceae; Chenopodium amaranticolor was a good local lesion host. Virus particles, trapped by immunosorbent electron microscopy and stained with uranyl acetate, were 710 ×10 nm long. Purified virus preparations contained a single polypeptide species of 32,500 Da and one nucleic acid species of 3.1 · 106 Da. The virus was serologically related to soybean mosaic, azuki bean mosaic and peanut mottle viruses but not to clover yellow vein, pea seed-borne mosaic and bean yellow mosaic viruses.On the basis of these properties, the virus was identified as a previously undescribed potyvirus in chickpea, for which the name chickpea bushy dwarf virus is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1989.tb01446.x
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  • 4
    Electronic Resource
    Electronic Resource
    A plant rhabdovirus associated with peanut veinal chlorosis disease in India (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A disease of peanut characterized by stunting of plants, veinal chlorosis, outward bending of leaflets, and proliferation of axillary buds has been observed in several parts of Peninsular India since 1977. The disease was restricted to peanut crops raised during the post-rainy season. A rhabdovirus was associated with this disease. This appears to be the first record of natural occurrence of a rhabdovirus in peanut.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1989.tb01462.x
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  • 5
    Electronic Resource
    Electronic Resource
    Purification, serology and physico-chemical properties of a peanut mottle virus isolate from India (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 32 (1983), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A procedure developed for the purification of peanut mottle virus (PMV) isolated from peanuts in India yielded 30–40 mg of virus/kg of plant tissue. Purified virus sedimented as a single component of 151 S. The virus coat protein migrated as a single component in each of two polyacrylamide gel concentrations and had an estimated molecular weight of 34 000 daltons. The molecular weight of the nucleic acid was 3.10 × 106 daltons. In the precipitin ring interface test (PRIT) PMV showed a serological relationship with soybean mosaic virus (SMV). Using the double antibody sandwich form of ELISA, PMV was shown to be distantly related to adzuki bean mosaic (ABMV), amaranthus leaf mottle (ALMV), clover yellow vein (CYVV) viruses and SMV. Immunosorbent electron microscopy (ISEM) showed PMV to be closely related to ABMV, ALMV, CYVV and SMV. In PRIT, ELISA and ISEM tests no specific serological reaction was noted between PMV and antisera to groundnut eye spot, peanut green mosaic, pepper veinal mottle, potato virus Y, sugarcane mosaic and turnip mosaic viruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1983.tb01319.x
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  • 6
    Electronic Resource
    Electronic Resource
    Peanut yellow spot virus is a member of a new serogroup of Tospovirus genus based on small (S) RNA sequence and organization (1998)
    Springer
    Archives of virology 143 (1998), S. 353-364 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. The sequence of the S RNA was 2 970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5′-large ORF (1 440 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16–21% sequence identity and 42– 48% sequence similarity with other tospoviruses. A 3′ ORF (741 nucleotides) in the virus complementary (vc) sense encoded a 28.0 kDa protein that was identified as the nucleocapsid (N) gene based on immunoblot analysis of the {in vitro} expressed protein with PYSV polyclonal antiserum. The predicted N protein had 24–28% amino acid sequence identity and 44–51% sequence similarity with the members of other serogroups. In contrast to other tospoviruses, a third ORF (204 nucleotides) occurred in the vc strand, which could encode a protein with a predicted size of 7.5 kDa with two strong hydrophobic regions. The low degree of homology of N and NSs protein sequences with other serogroup members coupled with an additional ORF suggests that PYSV should be classified as a distinct species of the Tospovirus genus. This conclusion also is supported by the absence of serological cross reaction with other serogroups, and biological characteristics including thrips transmission, symptoms and host range.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050291
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  • 7
    Electronic Resource
    Electronic Resource
    Peanut stripe virus in Indonesia (1989)
    Springer
    European journal of plant pathology 95 (1989), S. 123-127 
    Details
    ISSN: 1573-8469
    Keywords: Arachis hypogaea ; peanut mottle virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Tijdens een inspectie in 1986 en 1987 in Zuid Sulawesi, West en Oost Java en West Sumatra werd in aardnoot (Arachis hypogaea) veelvuldig een ziekte aangetroffen, die opviel door donkergroene vlekkerigheid. De vlekken waren onregelmatig over de bladeren verspreid of kwamen voornamelijk voor rond de nerven. Soms waren de vlekken omgeven door een lichtgroene ring. Op grond van symptomen op toetsplanten, serologische verwantschap en elektronenmicroscopische eigenschappen werd geconcludeerd, dat deze ziekte veroorzaakt wordt door het ‘peanut stripe virus’. Dit virus is voor het eerst beschreven in 1984 in Amerika. Dit is de eerste officiële melding van het virus in Indonesië. Enkele eerder beschreven potyvirussen van aardnoot in Indonesië, ‘groundnut mottle-y’ en ‘peanut mottle virus’ (PMV) zijn waarschijnlijk ook PStV. De gelijkenis van de door PStV and ‘peanut mottle virus’ veroorzaakte symptomen heeft ervoor gezorgd dat de vlekkerigheid op aardnoot in Indonesië jarenlang aan PMV werd toegeschreven. Aangezien PStV veel schade veroorzaakt, wordt verder onderzoek gedaan aan de epidemiologie van dit virus, aan het oogstverlies en aan het toetsen van aardnootlijnen op resistentie.
    Notes: Abstract During a survey of groundnut in 1986 and 1987 in South Sulawesi, West and East Java and West Sumatra a disease was frequently observed which is characterized by the presence of green blotches. These blotches were either randomly distributed over the entire leaflets or concentrated along the veins; sometimes they were surrounded by light green rings. On the basis of test plant studies, serology and electron microscopy it was concluded that the causal agent of the disease is peanut stripe virus (PStV). This virus has been described for the first time in the USA in 1984. Some potyviruses described earlier in Indonesia, viz. groundnut mottle-y and peanut mottle virus (PMV) probably are also PStV. The similarity of symptoms caused by PStV and peanut mottle virus was the reason why blotching on groundnut in Indonesia was ascribed for many years to PMV. As PStV causes severe yield losses, further research is being done on the epidemiology of the virus, on yield loss assesment and on testing breeding lines of groundnut for resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01997480
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  • 8
    Electronic Resource
    Electronic Resource
    Assessment of yield losses caused by chickpea chlorotic dwarf geminivirus in chickpea (Cicer arietinum) in India (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 221-224 
    Details
    ISSN: 1573-8469
    Keywords: chickpea stunt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Yield losses caused by chickpea chlorotic dwarf virus in chickpea were estimated by comparing uninfected and infected plants in the field at two locations in India. When infection was before flowering, yield losses of individual plants amounted to nearly 100% in the three cultivars studies. Plants that became infected during flowering had yield losses of 75–90%. Percentage of crop loss is likely to equal percentage of disease incidence, since plant densities in farmers' fields are probably too low to allow uninfected plants around infected ones to compensate the yield losses of infected plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874768
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  • 9
    Electronic Resource
    Electronic Resource
    Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses (1996)
    Springer
    Archives of virology 141 (1996), S. 85-98 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The complete nucleotide sequence of the S RNA of peanut bud necrosis virus (PBNV) has been determined. The RNA is 3 057 nucleotides in length, contains inverted repeats and two open reading frames (ORFs) with an ambisense coding strategy that are separated by an A+U-rich intergenic region. One ORF (1 320 nucleotides in the viral sense strand) encodes a Mr 49.5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein. This protein was identified as the nucleocapsid (N) protein based on sequence similarities. Amino acid sequence comparison of N and NSs proteins revealed identities of 22–34% with the reported tospovirus isolates of serogroups I, II, and III, whereas it had 82–86% identity with viruses in serogroup IV, watermelon silver mottle virus (WSMV) and tomato isolate of peanut bud necrosis virus (PBNV-To). Two subgenomic RNA species detected in PBNV infected tissue corresponded to the predicted sizes (1.65 and 1.4 kb) of the NSs and N mRNAs. The data presented show conclusively that PBNV should be included in serogroup IV, along with WSMV and PBNV-To.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718590
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  • 10
    Electronic Resource
    Electronic Resource
    The nucleotide sequence of RNA-1 of Indian peanut clump furovius (1996)
    Springer
    Archives of virology 141 (1996), S. 2301-2312 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The nucleotide sequence of RNA-1 of an isolate of the H serotype of Indian peanut clump virus (IPCV-H) was shown to comprise 5 841 nucleotides. The RNA contains three open reading frames (ORF) which are between nucleotides 133 and 3 522, nucleotides 3 526 and 5 103 (assuming expression by suppression of the ORF 1 termination codon) and nucleotides 5 168 and 5 539. The encoded polypeptides have M r , of 129 687 (p130), 60 188 (p60) and 14 281 (p14). ORF 2 is thought to be expressed by suppression of the termination codon of ORF 1 to produce a M r , 189 975 product (p190). p130 contains sequences characteristic of proteins with methyl transferase and NTP-binding properties and p190 contains these and sequences characteristic of RNA-dependent RNA polymerases. The nucleotide sequence of IPCV RNA-1 is similar to that of peanut clump virus (PCV) and corresponding encoded polypeptides are 88% (p130), 95% p60 and 75% (p14) identical. The sequences of the translation products are also similar to those of soil-borne wheat mosaic virus and barley stripe mosaic virus. Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718632
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