ISSN:
1432-8798
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Mice immunized with 3 or 5 doses of cell-free extract from adenovirus 12-induced tumour cells were relatively immune to subsequent challenge with 5 × 105 viable adenovirus 12-induced tumour cells. Using spleen cell transfer experiments, this immunity was shown to be cell mediated. Thus, subcutaneous inoculation with spleen cells from mice immunized with tumour extract together with tumour cells in a 15∶1 ratio, respectively, inhibited tumour growth, as shown by a reduction in tumour incidence and the size of tumours, compared to the control mice inoculated with tumour cells and normal mouse spleen cells. When the tumour cells and spleen cells were given by different routes of inoculation, inhibition was not as marked as when the two were given together. Mice inoculated with spleen cells, from mice immune to challenge with live tumour cells (tumour immune mice) or spleen cells from mice bearing large transplantable tumours, were shown to be relatively immune to transplanted tumour cells. Transfer of serum from mice immunized with tumour extract to normal mice did not confer any immunity to transplanted tumour cell challenge; tumour growth was neither retarded or enhanced, compared to control mice given normal CBA mouse serum. Transfer of serum from mice immune to tumour cell challenge also did not affect the growth of tumours. However, transfer of serum from tumour-bearing mice resulted in an apparent earlier development of tumours in two distinct experiments; however, in neither experiment was the difference statistically significant compared to the tumour incidence in control mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01249936
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