ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Immuno-labeling of human peripheral blood lymphocytes with either polyglutaraldehyde or polyacrolein microspheres was shown by analytical electrophoresis to generate two electrokinetically distinct sub-populations of lymphocytes. By means of free-flow electrophoresis, the high mobility fraction was found to consist predominantly of T lymphocytes using an anti-T cell antibody, while the low mobility fraction consisted of lymphocytes labeled with immunomicrospheres, identifying these cells as B lymphocytes. The immunomicrosphere-induced reduction of B-cell mobility below that of the T cells thus allowed the enrichment and recovery of viable T cells in preparative quantities by free-flow electrophoresis.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150030510
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