ISSN:
1432-072X
Keywords:
Peptostreptococcus productus (strain Marburg)
;
Homoacetogenic bacteria
;
Ferredoxin
;
Carbon monoxide dehydrogenase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Ferredoxin was purified to apparent homogeneity from cell extracts of the homoacetogen Peptostreptococcus productus (strain Marburg). The yield was 70 μg ferredoxin per g wet cells of P. productus. The UV-vis spectrum exhibited characteristics of a typical clostridial ferredoxin spectrum with a molar extinction coefficient ε385 of ∼30000 M-1 cm-1 and an A385/A280 ratio of 0.76. The molecular weight Mr was near 5700 as calculated from the amino acid composition. The protein contained per mol 9.9 mol iron, 8.2 mol acid-labile sulfide, and near 7 mol cysteine indicating the presence of two 4 Fe/4 S clusters. The redox potential was determined to be-410 mV. The purified ferredoxin was reduced with carbon monoxide by the carbon monoxide dehydrogenase from crude extracts and by the partially enriched enzyme of P. productus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00248721
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