ISSN:
1432-2048
Keywords:
Pollen (development)
;
Lilium (pollen development)
;
mRNA expression (pollen development)
;
Microgametophyte
;
Microspore
;
Nicotiana (pollen development)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Homogeneous populations of developing microspores and pollen from anthers of lily (Lilium longiflorum Thumb.) and tobacco (Nicotiana tabacum L.) show a continuous production of biomass, reaching a maximum in young pollen. The rate of RNA synthesis was 460 fg · h−1 in young binucleate cells, 138 fg · h−1 in late binucleate cells and 56 fg · h−1 in microspores. The mRNA population in developing pollen can be separated into three groups. In the first group, certain types of mRNAs are present at a constant level during all stages of development. A second group is characteristic of young pollen and increases quantitatively until anthesis. A third group is seen transiently; to this belong mRNAs present only before mitosis or at a distinct cell stage after mitosis. Some of the translation products of this latter group of mRNAs showed similarities between lily and tobacco on two-dimensional gels in respect of molecular weight and isolectric point, indicating that those mRNAs and proteins play a role in the regulation of pollen development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197125
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