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  • 1
    Electronic Resource
    Electronic Resource
    Ethylene and in vitro rooting of hazelnut (Corylus avellana) cotyledons (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 81 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ethylene may be one of the many factors that play a role in rooting. However, in some studies ethylene promoted rooting, while in others it was inhibitory or had no effect. Using cotyledons of hazelnut (Corylus avellana L. cv. Casina) observations were made of the effect of ethylene precursors on adventitious root formation. l-methionine (Met) or 1-aminocyclopropane-1-carboxylic acid (ACC) added to a standard indole-3-butyric acid (IBA)-kinetin-containing medium did not enhance rooting, while 2-chloroethylphosphonic acid (CEPA) did. The ethylene inhibitor, aminoethoxyvinylglycine (AVG), inhibited root formation, but its effect was reversed by ACC when cotyledonary segments were transferred to rhizogenic medium plus ACC at day 10. Ethylene production by cotyledons cultured on rhizogenic medium or rhizogenic medium plus CEPA was high at the beginning of rooting. Thus, the wound-induced ethylene is a key stimulatory factor in the formation of root primordia. The data support the hypothesis that ethylene plays a positive role in root formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02134.x
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  • 2
    Electronic Resource
    Electronic Resource
    Ethylene in relation to protein, peroxidase and polyphenol oxidase activities during rooting in hazelnut cotyledons (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During formation of adventitious roots, the effects of 2-chlorethylphosphonic acid (CEPA), 1-aminocyclopropane-1-carboxylic acid (ACC) and aminoethoxyvinylglycine (AVG) added to a Cheng basal medium, supplemented with indole-3-butyricacid (IBA) and kinetin were determined on peroxidase (PO; EC 1.11.1.7) and poiyphenol oxidase (PPO; EC 1.10.3.1) activities in cotyledon explants of hazelnut (Corylus avellana L. cv. Casina). CEPA stimulated PO and PPO activities while AVG inhibited. SDS-polyacrylamide gel electrophoresis of preparations from hazelnut cotyledons showed a correlation between proteins and rooting. Ethylene seems to modify total protein content and the activities of PO and PPO. As compared to the control extracts, AVG inhibited the anodic (53, 30.7 and 27 kDa) and cathodic (66.2 and 53.4 kDa) isoperoxidases and anodic (27.5 and 21 kDa) and cathodic (67.5 kDa) isopolyphenol oxidases, whereas CEPA promoted the anodic (30.7, 28.8 and 27 kDa) and cathodic (53.4 and 27.2 kDa) isozymes with PO activity. The increased PO activity during rooting in hazelnut cotyledons could enhance isozymes with lignin biosynthetic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb02477.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of repeated severe pruning on endogenous polyamine content in hazelnut trees (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 92 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Endogenous polyamine content was determined in leaves and buds of adult and repeatedly severe pruned hazelnut trees (Corvlus avellana L.). Polyamine content in leaves from shoots obtained by forced outgrowth of branches taken from adult and pruned trees was also determined. Variations of polyamine levels in relation to pruning treatments were observed in all the analysed, tissues. Free polyamines increased in response to pruning treatments, mostly due to an increase in free putrescine. Free spermidine and spermine seemed to decrease with pruning intensity, whereas bound polyamines did not seem to correlate with treatments. Significantly, in all the analysed tissues the putrescine to spermidine plus spermine ratio increased in the free polyamine fraction. The results indicate that polyamine metabolism could play a role as a physiological marker for juvenility and rejuvenation in relation to cloning of woody plants. The possible role of polyamines in mediating and/or regulating phase change and reinvigoration is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb08840.x
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  • 4
    Electronic Resource
    Electronic Resource
    Benzyladenine-controlled protein synthesis and growth in apple cell suspensions (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 84 (1992), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Prolific growth in cytokinin-requiring apple cell suspensions was achieved during 10 days of culture with appropriate concentrations of benzyladenine (BA). Unlike the controls, BA-treated cells showed a well developed sub-cellular organization and protein synthesis. Upon transfer to fresh cytokinin-free medium, however, these cells exhibited a rapid decrease in polyribosome formation, but with unchanged nuclear and nucleolar activities. Cells lacking added cytokinin showed a reduced metabolic activity and failed to divide. Deficiency in the translation process of cytokinin-deprived cells was overcome by exogenous BA-treatment. In cells maintained on a cytokinin-free medium for 5 days, BA-treatment enhanced protein synthesis from pre-existing mRNA in a specific way.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1992.tb04658.x
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison of endogenous polyamine content in hazel leaves and buds between the annual dormancy and flowering phases of growth (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 91 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Variations of endogenous polyamine levels were determined in leaves and buds of mature hazelnut trees (Corylus avellana). Results indicated specific correlations between high spermidine and spermine levels with rapid shoot growth and leaf expansion. Conversely, low spermidine and spermine, along with increasing putrescine levels, may be associated with the imposition of shoot-bud dormancy. In previous studies on the morphogenic ability in hazel, and taking into account that hazel explants can be easily established in vitro during dormancy imposition, we observed that high levels of putrescine and increasing putrescine to polyamines ratios could be related to a higher morphogenic potential in this species, showing in adult tissues the typical behaviour of juvenile tissues in vitro. Genotypic differences affecting the morphogenic potential could account for the observed differences in the polyamine levels of the cultivars. The data obtained could also be correlated with seasonal changes of other endogenous growth regulators in hazel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb00657.x
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  • 6
    Electronic Resource
    Electronic Resource
    Evaluation of climatic change through harmonic analysis (1994)
    Springer
    Natural hazards 9 (1994), S. 5-16 
    Details
    ISSN: 1573-0840
    Keywords: Fourier transform ; maximum entropy spectral analysis ; precipitation ; temperature ; climatic change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography , Geosciences
    Notes: Abstract In the present work, a precipitation and temperature series from Barcelona (Spain) are analysed in order to detect the possible existence of climatic changes or cycles. The analysis is carried out both from the temporal and spectral standpoints. The techniques used range from the classical periodogram and Blackman-Tukey method through to the Maximum Entropy method. The results do not show the existence of climatic cycles, though they do show a clear tendency toward increased precipitation and decreased temperature, since the last years of series.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662588
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  • 7
    Electronic Resource
    Electronic Resource
    Multiple shoot-bud formation and plantlet regeneration on Castanea sativa Mill. seeds in culture (1982)
    Springer
    Plant cell reports 1 (1982), S. 161-164 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Primordial initiation and development of shoot-buds has been accomplished by using shoots derived from chestnut (Castanea sativa Mill) seedlings cultured with added 6-benzylaminopurine (BAP). Germination of chestnut seeds in the presence of BAP (4 – 40 μM) stimulated varying numbers of shoot-buds in those areas of the main axis that were favorably altered. When excised single shoots from these treated seeds were subcultured on a fresh medium containing BAP (4 – 40 μM) continual shoot production was observed. Bud growth and shoot elongation were stimulated by transferring cultures to a reduced concentration of BAP (2 μM) plus indole-3-butyric acid (IBA 0.4 μM). Plant regeneration occurred in the presence of IBA (0.8 μM) after a preconditioning treatment in which naphthaleneacetic acid (NAA 50 μM) and kinetin (k 2 μM) were applied to the tissue culture shoots for 7 days in light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269188
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  • 8
    Electronic Resource
    Electronic Resource
    “In vitro” filbert (Corylus avellana L.) micropropagation from shoot and cotyledonary node segments (1985)
    Springer
    Plant cell reports 4 (1985), S. 137-139 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plantlets were successfully regenerated from shoot and cotyledonary node segments excised from 20 day old filbert seedlings. In both cases the optimum initiation and elongation of shoot buds was obtained after 15 days culture in 1/2K(h) medium plus BAP (25 μM) followed by 20 days culture in the same medium in presence of a reduced BAP concentration (0.5 or 2.5 μM). Maximum of functional roots were readily formed after 5 days of submersion of the basal end of shoots in 1/2K(h) liquid medium plus IBA (50 μM), then transferred to a fresh 1/2K(h) solid medium for a further 15 days. Following these two consecutive steps, root initiation and development was achieved in 80% of the explants. The histological origin of neoformed organs was studied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571300
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  • 9
    Electronic Resource
    Electronic Resource
    Factors involved in Agrobacterium tumefaciens-mediated gene transfer into Pinus nigra Arn. ssp. salzmannii (Dunal) Franco (2000)
    Springer
    Euphytica 114 (2000), S. 195-203 
    Details
    ISSN: 1573-5060
    Keywords: conifers ; salgareño pine ; tissue culture ; transformation ; transient gene expression ; uidA expression ; vir gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Cotyledons from dissected sterile embryos of salgareño pine (Pinus nigra Arn. ssp. salzmannii (Dunal) Franco) were inoculated with different disarmed Agrobacterium tumefaciens strains harbouring the binary vector p35SGUSint. The transient expression of a β-glucuronidase gene (uidA) was studied, using a histochemical staining procedure. Nineteen days after inoculation, the activity of β-glucuronidase was detected in epidermal and subepidermal layers of cotyledonary explants. The EHA105 strain harbouring a disarmed agropine-type Ti-plasmid (pTiBO542) was the most effective for gene transfer of the uidA gene. The effects of exudates and extracts from 0-day-old embryos on induction of vir gene expression in A. tumefaciens were also examined. The results of this study showed that salgarño pine embryo exudates contain a substance(s) that induce vir gene expression, in similar way to that observed with 100 μM acetosyringone (AS).All these findings suggest that T-DNA processing and transfer might take place when Agrobacterium infects suitable tissues of salgareño pine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003946131356
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  • 10
    Electronic Resource
    Electronic Resource
    Influence of hormones on DNA synthesis of breast tumors in culture (1986)
    Springer
    Breast cancer research and treatment 8 (1986), S. 223-232 
    Details
    ISSN: 1573-7217
    Keywords: human mammary tumors ; DNA synthesis ; tissue culture ; estrogen ; progesterone ; prolactin ; thymidine labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Tissue culture techniques have been developed for studying the influence of hormones on human breast tissues. The present study demonstrated that estrogen induced a highly significant increase in3H-thymidine incorporation into DNA and labelling index of ductal epithelium of fibrocystic disease; there was no effect of progesterone, either alone or in combination with ovine prolactin, on benign lesions. Estrogen stimulated certain malignant tumors derived from postmenopausal women. These studies also showed that there was an inhibitory effect of3H-thymidine incorporation into DNA by the effect of progesterone on malignant lesions. When menopausal status was considered, it was found that DNA synthesis was significantly higher in the presence of insulin and hydrocortisone in malignant tumors derived from premenopausal women than from postmenopausal women, or than in benign lesions. Thus, the present findings may provide evidence that specific activity may be an important measurement for breast tumor DNA synthesis in response to ovarian hormones or other substances.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01807335
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