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  • 1
    Electronic Resource
    Electronic Resource
    The induction of the “turbo reductase” is inhibited by cycloheximide, cordycepin and ethylene inhibitors in Fe-deficient cucumber (Cucumis sativus L.) plants (1998)
    Springer
    Protoplasma 205 (1998), S. 156-162 
    Details
    ISSN: 1615-6102
    Keywords: Cucumis sativus ; Ethylene ; Ferric-reducing capacity ; Iron deficiency ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dicotyledonous plants respond to Fe deficiency by enhancing the capacity of their roots to reduce Fe(III) to Fe(II). It has been suggested that there are two different ferric redox systems in the roots: the standard reductase, active with ferricyanide and not inducible by Fe deficiency, and the turbo reductase, active with both ferricyanide and ferric chelates and inducible by Fe deficiency. We have used different experimental approaches to test whether or not the Fe(III)-reducing capacity of cucumber (Cucumis sativus L. cv. Ashley) roots can be explained by considering the standard and the turbo reductase as the same enzyme. For this, we used both Fe-sufficient and Fe-deficient plants, which were treated with ethylene inhibitors (cobalt or silver thiosulfate; found to inhibit the turbo reductase in a previous work), a protein synthesis inhibitor (cycloheximide), or an mRNA polyadenylation inhibitor (cordycepin). At different times after application of these inhibitors, reduction of both ferricyanide and Fe(III)-EDTA were determined. In addition, we studied the effects of pH and temperature on the reduction of ferricyanide and Fe(III)-EDTA by both Fe-sufficient and Fe-deficient plants. Results suggest that there are, at least, two different ferric redox systems in the roots. Enhancement of Fe(III)-reducing capacity (turbo reductase) by Fe-deficient plants probably requires the de novo synthesis of a (or several) protein(s), which has a high turnover rate and whose expression is presumably regulated by ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279305
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution (1991)
    Springer
    Plant and soil 130 (1991), S. 115-119 
    Details
    ISSN: 1573-5036
    Keywords: bicarbonate ; iron chlorosis ; nurtrient solution ; phosphate ; Prunus amygdalus × P. persica ; P. domestica ; P. persica ; rootstocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Iron chlorosis is an important problem in peach trees, but differences exist between peach rootstocks in their tolerance to Fe chlorosis in calcareous soils. The purpose of this investigation was to characterize the tolerance of different rootstocks to Fe chlorosis induced by bicarbonate in nutrient solution. The rootstocks studied included peach (Nemaguard), plums (Brompton, San Julian A and Puebla de Soto 101) and almond × peach hybrids (Adafuel and GF677). Young plants obtained from rooted cuttings or from in vitro culture techniques were grown individually, under controlled conditions, in flasks with 700 mL of aerated nutrient solution low in iron and with or without 10 mM bicarbonate or 10 mM phosphate. Susceptiblity to bicarbonate-induced chlorosis was inversely correlated with both the Fe content in young leaves and the reducing capacity of roots, but not with the phosphorus content in young leaves. The plum Puebla de Soto 101 and the hybrid GF677 showed the lowest degree of chlorosis and the highest reducing capacity. Phosphate did not induce chlorosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011865
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the tolerance to iron chlorosis in different peach rootstocks grown in nutrient solution (1991)
    Springer
    Plant and soil 130 (1991), S. 121-125 
    Details
    ISSN: 1573-5036
    Keywords: iron chlorosis ; iron-stress response mechanisms ; nutrient solution ; Prunus amygdalus x P. persica ; P. domestica ; P. persica ; rootstocks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The response mechanisms to Fe chlorosis have been widely studied in herbaceous plants and qulitative and quantitative differences have been found among species and among genotypes within the same species. Similar studies on woody plants are scarce. The objective of this work was to characterize specific response mechanisms in peach rootstocks with differing tolerances to Fe chlorosis in calcareous soils and to determine where acidification and reduction occur along the root system. Plants were grown individually, in growth chamber, in flasks with 700 mL of aerated nutrient solution with or without Fe. Without Fe, the peach Nemaguard was the most chlorotic, developed the least reducing capacity and mobilized less Fe and Mn from roots to young leaves compared to the other rootstocks. The pH decrease of the nutrient solution with Fe-deficiency was significant only in the plum rootstocks. Using an agar technique, both responses, reducing capacity and acidification, were located at the subapical zones of the roots, although no swollen root tips were apparent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011866
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  • 4
    Electronic Resource
    Electronic Resource
    B-chromosomes in inbred lines of rye (Secale cereale L.) (1996)
    Springer
    Genetica 97 (1996), S. 65-72 
    Details
    ISSN: 1573-6857
    Keywords: Secale cereale ; rye ; B chromosomes ; inbred lines ; pairing ; pollen mitosis ; transmission genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract B chromosomes from an experimental population of the Japanese JNK strain of rye, isogenic for its Bs, have been backcrossed into twelve different inbred lines. The experiment is a way of studying the effects of the Bs against a range of different homozygous A chromosome backgrounds. This publication deals with pairing effects of both the As and the Bs, and their interactions, and with pollen mitosis. At meiosis there is a genotypic component to B effects, and they do not appear to act solely through a physical disturbance within the nucleus. In pollen the Bs are always present in more than 50% of the grains regardless of their pairing behaviour during meiosis; this result fits with a ‘parasitic’ model of the activity of rye Bs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00132582
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