ISSN:
1600-0765
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated ammo-acids. Increasing concentrations of minocycline (10, 50, 100μg/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100μg/ml which statistically significantly (p 〈 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 μg/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 fig/ml of minocyline in the “attachment medium” induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10μg/ml minocycline-HCl solution increased significantly (p〈0.005) cell migation towards a gradient of fetal calf serum. The presence of 10 μg/ml of minocycline in contact with the kerationocytes in the upper compartment of the migration chambers also produced a significant (p 〈 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemo attractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyoud50μg/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of junctional epithelium.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0765.1998.tb02213.x
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