ISSN:
0741-0581
Keywords:
Immunocytochemistry
;
Electron microscopy
;
Colloidal gold
;
Glial fibrillary acidic protein
;
Neuron-specific enolase
;
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Natural Sciences in General
Notes:
A postembedding immunocytochemical technique is described that allows ultrastructural localization of glial fibrillary acidic protein and neuron-specific enolase on tissues originally processed only for routine light and electron microscopy. Use of the oxidizing agent sodium metaperiodate prior to incubation with the primary antiserum sufficiently removes osmium tetroxide (OsO4) from potential antigen - antibody combining sites to allow specific localization of these neural antigens by colloidal gold immunolabelling. Both human and monkey neural tissues, prepared for routine ultrastructural examination with aldehyde fixatives and OsO4 postfixation, show excellent ultrastructural morphology and antigen localization. In addition, formalin-fixed, paraffin-embedded pathological human brain tissues, obtained at autopsy up to 22 years previously, show good ultrastructural immunolocalization of glial fibrillary acidic protein when re-embedded for electron microscopy. Thus, ultrastructural immunolocalization of certain neural antigens is easily achieved in tissues originally processed for routine light and electron microscopy. This allows re-examination of archival tissues using current immunocy-tochemical advances, including that of selected pathological tissues previously prepared solely for light microscopy.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jemt.1060050109
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