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  • 1
    Electronic Resource
    Electronic Resource
    The use of Escherichia coli bearing a phoN gene for the removal of uranium and nickel from aqueous flows (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 266-272 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A Citrobacter sp. originally isolated from metal-polluted soil accumulates heavy metals via metal-phosphate deposition utilizing inorganic phosphate liberated via PhoN phosphatase activity. Further strain development was limited by the non-transformability of this environmental isolate. Recombinant Escherichia coli DH5α bearing cloned phoN or the related phoC acquired metal-accumulating ability, which was compared with that of the Citrobacter sp. with respect to removal of uranyl ion (UO2 2+) from dilute aqueous flows and its deposition in the form of polycrystalline hydrogen uranyl phosphate (HUO2PO4). Subsequently, HUO2PO4-laden cells removed Ni2+ from dilute aqueous flows via intercalation of Ni2+ into the HUO2PO4 lattice. Despite comparable acid phosphatase activity in all three strains, the E. coli DH5α (phoN) construct was superior to Citrobacter N14 in both uranyl and nickel accumulation, while the E. coli DH5α (phoC) construct was greatly inferior in both respects. Expression of phosphatase activity alone is not the only factor that permits efficient and prolonged metal phosphate accumulation, and the data highlight possible differences in the PhoN and PhoC phosphatases, which are otherwise considered to be related in many respects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051288
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  • 2
    Electronic Resource
    Electronic Resource
    Bacterial nonspecific acid phosphohydrolases: physiology, evolution and use as tools in microbial biotechnology (1998)
    Springer
    Cellular and molecular life sciences 54 (1998), S. 833-850 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Acid phosphohydrolases; bacteria; genetics; physiology; molecular evolution; microbial biotechnology.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Bacterial nonspecific acid phosphohydrolases (NSAPs) are secreted enzymes, produced as soluble periplasmic proteins or as membrane-bound lipoproteins, that are usually able to dephosphorylate a broad array of structurally unrelated substrates and exhibit optimal catalytic activity at acidic to neutral pH values. Bacterial NSAPs are monomeric or oligomeric proteins containing polypeptide components with an M r of 25 – 30 kDa. On the basis of amino acid sequence relatedness, three different molecular families of NSAPs can be distinguished, indicated as molecular class A, B and C, respectively. Members of each class share some common biophysical and functional features, but may also exhibit functional differences. NSAPs have been detected in several microbial taxa, and enzymes of different classes can be produced by the same bacterial species. Structural and phyletic relationships exist among the various bacterial NSAPs and some other bacterial and eucaryotic phosphohydrolases. Current knowledge on bacterial NSAPs is reviewed, together with analytical tools that may be useful for their characterization. An overview is also presented concerning the use of bacterial NSAPs in biotechnology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050212
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  • 3
    Electronic Resource
    Electronic Resource
    Antibody response to wild rubella virus structural proteins following immunization with RA 27/3 live attenuated vaccine (1988)
    Springer
    Archives of virology 101 (1988), S. 25-33 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Antibody response to individual structural proteins (E 1, E 2, and C) of the M-33 wild rubella virus strain was assayed by an immunoblot technique in 12 girls, following immunization with RA 27/3 live attenuated rubella vaccine. Of the 12 immunized subjects, before vaccination 9 had no demonstrable rubella specific antibodies while the remaining 3 had a low level of rubella specific antibodies, reacting only with the E 1 protein. At one month after vaccination all the immunized subjects presented anti-E 1, anti-E 2, and anti-C specific antibodies. However, at 1–2 weeks after vaccination the 9 girls who were seronegative before immunization still had no detectable antibodies to any of the rubella virus structural proteins, while the 3 subjects whose preimmunization sera had reacted with the E 1 protein presented an accelerated immune response, showing anti-E 2 and anti-C specific antibodies and a more intensely marked anti-E 1 specific band.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314649
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  • 4
    Electronic Resource
    Electronic Resource
    Production of serum-opacity factor by Streptococcus pyogenes strains isolated from pharyngitis in children prevalence of ≪rheumatogenic≫ M types among of-negative strains (1985)
    Springer
    European journal of epidemiology 1 (1985), S. 37-41 
    Details
    ISSN: 1573-7284
    Keywords: S. pyogenes serum-opacity factor ; Rheumatogenic M types ; S. pyogenes M types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two hundred ten S. pyogenes strains isolated in 1979, 1980 and 1984 from children with pharyngitis were here examined for properties which might be relevant to their rheumatogenic potential. Strains were first tested for the production of streptococcal serum-opacity factor and, among those scored as OF-negative, the presence was then verified of M types which have been epidemiologically related to rheumatic fever. Members of ≪rheumatogenic≫ M types are present among strains causing pharyngitis in children; which, however, also include a considerable proportion of OF-positive, probably non-rheumatogenic, strains. The results are discussed in the light of the low incidence of rheumatic fever in this country.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00162310
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  • 5
    Electronic Resource
    Electronic Resource
    Prevalence of streptococcus mutans and dental decay in school children from Siena (Italy) (1989)
    Springer
    European journal of epidemiology 5 (1989), S. 189-192 
    Details
    ISSN: 1573-7284
    Keywords: Streptococcus mutans ; Dental decay ; Oral hygiene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Epidemiological indexes of dental decay and prevalence of Streptococcus mutans in dental plaque were studied in a group of 255 schoolchildren aged 6–11 from a suburban area near Siena (Italy). Mutans streptococci were isolated from 17.4% of subjects: a significant correlation with dental decay indexes and with the presence of active caries was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00156828
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular epidemiology of nasopharyngeal corynebacteria in healthy adults from an area where diphtheria vaccination has been extensively practiced (1992)
    Springer
    European journal of epidemiology 8 (1992), S. 560-567 
    Details
    ISSN: 1573-7284
    Keywords: Corynebacteria ; Molecular epidemiology ; Italy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In addition to conventional biochemical tests, a DNA probe specific for Corynebacterium diphtheriae was used to characterize 53 cystinase-positive and urease-negative corynebacteria strains isolated from pharyngeal and nasal swabs obtained from 515 healthy adults living in an urban area of central Italy. No Corynebacterium diphtheriae strain was found. Six “atypical” strains were isolated, which could not be classified in any of the species so far defined in the Corynebacterium genus. These strains appeared to be biochemically close to Corynebacterium pseudodiphtheriticum and genetically close to Corynebacterium diphtheriae, since their DNAs strongly hybridized, under relatively low stringency conditions, with a Corynebacterium diphtheriae-specific probe and since insertion sequences which are usually found in Corynebacterium diphtheriae genomes were also found to be present in their genomes. No one of these six strains was either toxigenic or susceptible to lysogenization by β-corynephage carrying the tox gene. Therefore, they do not seem to have any epidemiological relevance as possible hosts for β-phages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146377
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  • 7
    Electronic Resource
    Electronic Resource
    Carbapenem-Hydrolyzing Metallo-Beta-Lactamases in Pseudomonas aeruginosa (1999)
    Springer
    European journal of clinical microbiology & infectious diseases 18 (1999), S. 455-456 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100960050320
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