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  • 1
    Electronic Resource
    Electronic Resource
    Push-out strength and SEM evaluation of resin composite bonded to internal cervical dentin (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Dental traumatology 12 (1996), S. 0 
    Details
    ISSN: 1600-0595
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The bond strength of a resin composite used with a dual-cured dentin bonding system to internal cervical bovine dentin was evaluated using a direct or indirect placement technique. Teeth were sectioned transversely to produce 4 mm-thick specimens. The root canals were enlarged to a standardized taper, treated with a dentin bonding system, and filled with a lightcured resin composite using either direct, incremental composite placement or indirect composite placement of a pre-polymerized composite inlay. The debond stress of indirectly placed restorations using a composite inlay was 8.5 (SD±2.7) MPa which was significantly greater (p〈0.0001) than the value of 5.0 (SD±1.9) MPa for composite placed in a conventional, incremental manner. SEM evaluation revealed the indirect placement technique demonstrated increased resin tag density and length as compared to the direct technique. Enhanced retention of resin composite to endodoutically prepared dentin treated with a dentin bonding system was obtained by using a composite inlay technique as opposed to direct, incremental buildup of the material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-9657.1996.tb00520.x
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  • 2
    Electronic Resource
    Electronic Resource
    Cell lipid alterations resulting from prolonged exposure to dimethylaminoethylmethacrylate (1999)
    Springer
    Clinical oral investigations 3 (1999), S. 181-187 
    Details
    ISSN: 1436-3771
    Keywords: Key words Methacrylates ; Oral epithelium ; Lipids ; Dental resins ; Biocompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Dimethylaminoethylmethacrylate (DMAEMA), a commonly-used component of visible-light polymerized dental resins, has the potential to elute and interact with tissue cells to cause cytotoxicity or sublethal metabolic changes. Short-term exposure of cultured oral epithelial cells to sublethal DMAEMA concentrations has been shown previously to affect cell neutral lipid and phospholipid metabolism, resulting in accumulation of significant quantities of dimethylphosphatidylethanolamine (DMPE). In non-treated cells, DMPE is a transient intermediate in phospholipid metabolism and is not detectable by standard methods. In the current study, the effects of prolonged exposure of cells to DMAEMA, and the mechanisms for formation of DMPE in the presence of DMAEMA were examined. Exposure of a keratinizing hamster buccal cheek pouch cell line (HCP cells) to 0.8 mM DMAEMA for 2, 3, 7, and 14 days resulted in reduced incorporation of [14C]acetate into several classes of phospholipids. DMPE was detectable at all time points in DMAEMA-exposed cultures and comprised between 12.48% and 18.33% of the total radiolabeled phospholipids. The results of short-term exchange experiments indicated that headgroup exchange was not the major reaction responsible for formation of DMPE in DMAEMA-treated cells; rather the formation appeared to occur through typical phospholipid metabolic pathways. The cells appeared able to re-establish and maintain homeostasis in the presence of this altered cell lipid composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007840050099
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials (1999)
    Springer
    Clinical oral investigations 3 (1999), S. 107-113 
    Details
    ISSN: 1436-3771
    Keywords: Key words Bisphenol A ; Xenoestrogen ; Dental restorative materials ; Bisphenol A dimethacrylate ; HPLC ; MCF-7
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007840050087
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro cytotoxicity of resin-containing restorative materials after aging in artificial saliva (1999)
    Springer
    Clinical oral investigations 3 (1999), S. 144-149 
    Details
    ISSN: 1436-3771
    Keywords: Key words Cell culture ; Resin composite ; Compomer ; Biocompatibility ; High-pressure liquid chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Studies have reported that dental resin-based materials release substances which have biological liabilities. However, some current methods for detecting these substances may not be adequate to detect biologically relevant concentrations. In the current study, we hypothesized that resin-based materials exhibit cytotoxic effects and alter cellular function in vitro when high-pressure liquid chromatography (HPLC-UV detection) cannot detect any release of substances. We further hypothesized that this release continues even after aging the samples in artificial saliva. Five types of composite or compomer materials (Z-100, Tetric Ceram, Dyract AP, Solitaire, and Clearfil AP-X) and one organically modified ceramic material (Definite) were tested after aging in artificial saliva for 0, 7, or 14 days. Cytotoxicity was assessed using direct contact with fibroblasts and measurement of succinic dehydrogenase activity after 48 h of exposure post aging. Release of substances from the materials was assessed using HPLC with UV detection. Altered cellular function was estimated by measuring proliferation of MCF-7 cells with sulforhodamine staining. HPLC showed that whereas initial release of substances was higher without aging, this release dropped significantly after 7 or 14 days of aging, and was equivalent to the Teflon controls after 14 days for four of the materials (Tetric Ceram, Definite, Solitaire, and Clearfil AP-X). Without aging in saliva, all materials had cytotoxicities 〉50% of the Teflon negative controls. After 14 days of aging, all materials except the Definite continued to show severe cytotoxicity. Only the Definite could be tested for its ability to alter cellular function because of the continuing toxicity of the other materials. This modified ceramic material caused a significant proliferative effect on the MCF-7 cells indicating that sufficient substances were released to alter cellular function. We concluded that all of these commercially available resin-based dental materials continue to release sufficient components to cause lethal effects or alter cellular function in vitro even after 2 weeks of aging in artificial saliva.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007840050093
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis and characterization of a monofunctional analog to BIS-GMA: A dental monomer (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 57 (1995), S. 705-716 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A new monofunctional BIS-GMA monomer, 4-(2-phenyl isopropyl)-3-phenoxy-2-hydroxy propyl methacrylate (monofunctional-BIS-GMA, hereafter abbreviated as MF-BIS-GMA) was synthesized as an adduct of 4-cumylphenol and glycidyl methacrylate for use as a dental monomer. The new monomer was characterized by FTIR, 1H, 13C (attached proton test, APT), UV, HPLC, and GPC. The viscosity and solubility of the new resin are also presented. The results were compared with the difunctional analog, BIS-GMA (2,2-bis[4-(2-hydroxy-3-methacryloyloxypropoxy)]phenyl propane), which is commercially available and currently used in dental restorative materials. Both monomers were light-cured using 0.3% camphorquinone and 0.75% 2-(dimethylamino)ethyl methacrylate as photoinitiators. The extent of monomer conversion and the potential for residual monomer leachability were compared between the two cured resins. The monofunctional resin was found to yield higher monomer conversion values (74 vs. 39%) and lower leachable components (0.03 vs. 30.6 mol %) than those of the difunctional analog. © 1995 John Wiley & Sons, Inc.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1995.070570606
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