ISSN:
1573-5028
Keywords:
Agrobacterium tumefaciens
;
crown gall
;
somaclonal variation
;
T-DNA rearrangements
;
tissue culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract After three years of apparent stability in tissue culture, the single cell derived shooty crown gall line sNT1.013 produced a revertant shoot which had switched from non-rooting (Rod+) and octopine synthesizing (Ocs+) to Rod- Ocs-, indicating that in this revertant TL-DNA genes 4 (causing the Rod+ trait) and gene 3 (causing the Ocs+ trait) had been inactivated. Southern blots revealed that the inactivation of these T-DNA genes was the result of a considerable rearrangement of DNA sequences, accompanied by deletions and possibly also by DNA amplifications. This study for the first time unambiguously proves that foreign genes which have been introduced via Agrobacterium tumefaciens can, at a low frequency, be inactivated after T-DNA integration because of reorganization of T-DNA sequences during tissue culture. This can be considered as an event of somaclonal variation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00017986
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