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  • 1
    Electronic Resource
    Electronic Resource
    The role of ecdysone in the induction and maintenance of hsp27 transcripts during larval and prepupal development of Drosophila (1996)
    Springer
    Development genes and evolution 206 (1996), S. 326-332 
    Details
    ISSN: 1432-041X
    Keywords: Key words hsp27 ; Ecdysone ; EcREs ; Developmental regulation ; Hormonal response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Drosophila melanogaster small heat-shock gene hsp27 carries the canonic ecdysone response element (EcRE) at -537. This EcRE has been used extensively both in cultured cells and in vitro for studies of the ecdysone response. We have characterised the developmental expression of hsp27 in wild-type larval and prepupal salivary glands in parallel with that of the E74B and E74A primary ecdysone response transcripts, which are induced in the mid and late third larval instar by a minor and major increase in ecdysone titre respectively. The induction of hsp27 occurs between these two events in larvae and in parallel with that of E74A in prepupae. Transcript levels are severely reduced in ecd1 and dor 22 (deep orange) mutant larvae but are only moderately reduced in larvae mutant for the Broad-Complex allele br t435 . By culturing salivary glands of different ages with low (1.8×10–8 M) or high (1.8×10–6 M) concentrations of hormone, we show that the response of an EcRE varies during development and that the timing of the response cannot be predicted solely from its apparent strength in cell line analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050059
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  • 2
    Electronic Resource
    Electronic Resource
    Regulatory elements and interactions in the Drosophila 68C glue gene cluster (1989)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 10 (1989), S. 189-197 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; transformation ; Glue gene ; Gene cluster ; Gene regulation ; Sgs-3 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We reviewed studies on the developmental regulation of the 68C glue gene cluster of Drosophila melanogaster. Extensive transformation analyses of Sgs-3 have shown that four regions necessary for normal expression can be distinguished. The first ( + 10 to -50) contains the transcription start site and TATA motif. This region can be replaced functionally by corresponding sequences from the hsp70 gene, but it is sensitive to point mutations in the TATA sequence. The second region (-50 to -98) contains more than one upstream sequence that, in combination with the other elements, leads to stage and tissue-specific expression. The third region (centered at -600) contains an element that enhances transcript levels some 20-fold. The final region (between -1.65 and -2.35 kb) contains elements having modest (twofold to threefold) effects on expression, one of which is contained in the coding sequences of Sgs-7, a second member of the cluster.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020100308
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    Paper (German National Licenses)
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