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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative determination of changes induced by NaCl in vacuoles and cellular size of Lycopersicon esculentum root cells (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 15 (1992), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Changes induced by NaCl in the sizes of both cells and vacuolar compartments of tomato seedling roots were determined using morphometric and stereological analysis. Parameters evaluated showed that cellular volume was smaller in roots grown under saline conditions than in control roots. This reduction was greater when NaCl concentration was increased. Similar behaviour can be observed in the vacuolar compartment where high concentrations of NaCl produce a reduction in vacuolar volume although the number of vacuoles per cell does not change under saline conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3040.1992.tb02156.x
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  • 2
    Electronic Resource
    Electronic Resource
    Decreased monosaccharide transport in renal brush-border membrane vesicles of spontaneously hypertensive rats (2000)
    Springer
    Cellular and molecular life sciences 57 (2000), S. 165-174 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Sugar transport; SHR; kidney; rat; BBMV.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Na+-dependent D-glucose and D-galactose transport were studied in brush-border membrane vesicles (BBMVs) from kidney cortex isolated from both spontaneously hypertensive rats (SHR) and their normotensive genetic control Wistar-Kyoto (WKY) rats. Initial rates and accumulation ratios of Na+-dependent D-glucose and D-galactose transport were significantly lower in SHR compared with WKY, the observed decreases being similar for both substrates. To explain the reduction in sugar transport by renal BBMVs, the density of Na+-dependent sugar cotransporters was studied in BBMVs from kidney cortex isolated from SHR and WKY rats. Phlorizin-specific binding and Western blot analysis indicated a reduction in the density of the cotransporters in SHR relative to WKY rats. This reduction was similar to those found for the initial rates and accumulation ratios for D-glucose and D-galactose in SHR. Na+ uptake, studied using 22Na+, was significantly increased in SHR, so the observed reduction in sugar transport could be due to disruption of the Na+ gradient between renal BBMVs in SHR. Furthermore, a significant decrease in the activity of Na+-K+-ATPase was observed in SHR. In conclusion, changes in the density of the Na+-dependent sugar cotransporter and in the Na+ gradient across the brush-border membranes might be involved in the observed reduction in sugar transport by renal BBMVs from SHR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000180050506
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  • 3
    Electronic Resource
    Electronic Resource
    Cytochemical localization of K+-dependent p-nitrophenyl phosphatase and adenylate cyclase by using one-step method in human washed platelets (1992)
    Springer
    Histochemistry and cell biology 97 (1992), S. 503-507 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural cytochemical localization of ouabain-sensitive, potassium dependent p-nitrophenyl phosphatase (K+-NPPase) of the Na+-/K+-ATPase complex and adenylate cyclase (cAMPase) activities, in washed inactivated human platelets, are described. The one-step lead-citrate method, under similar incubation conditions, was used to determine both activities. K+-NPPase appeared in both plasma membrane and the surface-connected canalicular system (SCCS) of the platelets. These data suggest a uniform distribution of the enzyme throughout membrane systems which are in contact with the external medium. cAMPase activity was strictly localized in tubules of the dense tubular system (DTS) when incubation medium contained prostaglandin E1, prostaglandin D2 or forskolin, at concentrations known to stimulate the enzyme in platelets that are intact. This fact and the inhibition of cytochemical reaction by thrombin confirm that the one-step lead-citrate method is a useful procedure in determining adenylate cyclase, abolishing the unfavorable conditions of previously reported methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00316071
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  • 4
    Electronic Resource
    Electronic Resource
    Light microscopic characterization of glycoconjugates in secretory cells of the carp (Cyprinus carpio) gill epithelium (1987)
    Springer
    Histochemistry and cell biology 88 (1987), S. 65-73 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Secretory products of granular and mucous cells in the gill epithelium of the carp, Cyprinus carpio, were distinguished by their cytochemical reactions with peroxidase-labelled lectins and with the galactose oxidase (GO)-Schiff reagents. Secretory products of granular cells reacted with lectins from Triticum vulgaris (WGA), Arachis hypogaea (PNA), Dolichos biflorus (DBA), Glycine max (SAB), and Lotus tetragonolobus (LTA). They also reacted with GO-Schiff reagents. After sialic acid cleavage with HCl, new binding sites for DBA and SBA appeared, suggesting the terminal sequence sialic acid-N-acetylgalactosamine (SA-GalNAc) for the secretion of this cell type. In mucous cells, binding sites for WGA, DBA, and SBA and, after acid hydrolysis, binding sites for PNA and a positive GO-Schiff reaction were detected. The terminal trisaccharide sialic acid-galactose (β1-3)-N-acetylgalactosamine (SA-Gal-GalNAc) is proposed for the secretion of mucuous cells. These cytochemical differences are discussed in light of the involvement of both cell types in fish mucus elaboration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490170
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  • 5
    Electronic Resource
    Electronic Resource
    Apical ouabain-sensitive K+-activated-ATPase activity in colon and caecum of the chick (1996)
    Springer
    Pflügers Archiv 433 (1996), S. 330-335 
    Details
    ISSN: 1432-2013
    Keywords: Key words K+-ATPase ; Intestine ; Epithelia ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study sought to investigate the presence and characteristics of K+-ATPase activity in chicken intestinal epithelia. A cytochemical method revealed Na+-independent, ouabain-sensitive, K+-ATPase activity in the apical, but not in the basolateral, membrane of chicken colonic and caecal epithelial cells. K+-ATPase activity was not observed in the small intestine. The measurement of K+-activated pNPPase activity was used to characterize the K+-ATPase activity evidenced by the cytochemical method. In addition, K+ and NH4 +, but neither Na+ nor Li+, could activate pNPPase activity in chicken intestinal epithelia. Vanadate abolished ouabain-sensitive, K+-activated pNPPase activity in the three membrane preparations tested, whereas oligomycin and SCH 28080 were without effect. The K m for K+ and the ouabain IC50 values for the apical colonic and caecal K+-activated pNPPase activity were higher than those measured for K+-activated pNPPase activity measured in the basolateral membrane of chicken jejunal enterocytes. The results indicate that the apical membranes of chicken colon and caecum possess Na+-independent, ouabain-sensitive K+-activated-ATPase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240050284
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on Ca2+-accumulating vesicles in oocytes of the snail Helix aspersa (1989)
    Springer
    Cell & tissue research 257 (1989), S. 597-601 
    Details
    ISSN: 1432-0878
    Keywords: Calcium, localization ; Oocytes ; Helix aspersa (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural, cytochemical and autoradiographic techniques were used to study the functional significance of Ca2+-accumulating vesicles in Helix aspersa oocytes. The organic material of these vesicles is capable of adsorbing calcium ions, thus removing them from the cytosol and forming osmotically inactive complexes that are concentrated in the core. Ca2+-accumulating vesicles likely act as subcellular detoxifying compartments that sequestrate excess cytosolic Ca2+ (and plausibly other metal cations) to maintain physiologically adequate ionic concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221470
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