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  • 1
    Electronic Resource
    Electronic Resource
    Phenotype and Cytokine Profile of Intraepithelial Lymphocytes in Human Small and Large Intestine (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 756 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb44544.x
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  • 2
    Electronic Resource
    Electronic Resource
    Presence of Human 65 kD Heat Shock Protein (hsp) in Inflamed Joints and Subcutaneous Nodules of RA Patients (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 31 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Monoclonal antibodies to the human homologue of the bacterial 65 kD heat shock protein (hsp) were used to investigate the tissue distribution of endogenous hsp 65 in normal versus rheumatoid synovial tissue, in subcutaneous nodules of patients with rheumatoid arthritis (RA) and in several instances of non-rheumatoid inflammation. A strong reactivity of the anti-hsp antibody was found in the cartilage-pannus junction in rheumatoid joints and in rheumatoid nodules, but not in normal joints or in normal or inflamed kidney or liver (irreversible graft rejection, chronic glomerulonephritis or primary biliary cirrhosis). The findings provide a new hypothetical explanation for a role of T cells reactive with the 65 kD hsp in the generation of both articular and extra-articular lesions in chronic rheumatoid arthritis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02770.x
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  • 3
    Electronic Resource
    Electronic Resource
    Synovial Cells Responding to a 65-kDa Mycobacterial Heat Shock Protein have a High Proportion of a TcRγδ Subtype Uncommon in Peripheral Blood (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 32 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We have analysed the ability of T cells from synovial fluid mononuclear cells (SFMC) and from peripheral blood mononuclear cells (PBMC) of inflammatory arthritic diseases to proliferate in response to mycobacterial antigens (65-kDa heal shock protein [hsp] of BCG. whole BCG) and to rat collagen type II. The SFMC demonstrated a significantly greater ability to respond to 65-kDa hsp of BCG. and to whole BCG, compared with PBMC from the same patients, With collagen type II, only a small proportion of the patients showed a proliferative response, although with this antigen also SFMC responded better than PBMC. There was no difference between SFMC and PBMC in the response to control antigen (tetanus toxoid), phytohaemagglutinin (PHA), or interleukin 2 (IL-2). A high proportion of cells in SFMC-derived short-term T-cell lines were of TcRγδ type, often exceeding the number of TcRγδ type. There was a significantly higher proportion of TcRγδ cells in the SFMC lines compared with the PBMC lines, and a large part of the TcRγδ cells in she SFMC cultures was CD8+ The SFMC lines had a high proportion of δ- TCS-1+ cells (Vδ1) among their TcRγδ cells, always exceeding the percentages of TiγA+ (Vγ9) and BB3+ (Vδ2) in the PBMC lines, the distribution of TcRγδ subtypes was markedly different, with a TiγA+/BB3+ population in the majority. These data argue for a different subpopulation distribution of TcRγδ cells in synovial fluid compared with peripheral blood of patients with inflammatory arthritic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb03191.x
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  • 4
    Electronic Resource
    Electronic Resource
    A Monoclonal Antibody to the Mycobacterial 65kDa Heat Shock Protein (ML 30) Binds to Cells in Normal and Arthritic Joints of Rats (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 33 (1991), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A monoclonal antibody reactive with the mycobacterial 65 kDa heal shock protein (ML 30) was investigated for reactivity with biopsies from normal rat joints and with inflamed joints due to adjuvant arthritis (AA) or collagen induced arthrilis (CIA).Immunohistochemical stainings with the ant-hsp 65 antibody on paralVm sections from normal rat joints revealed a weak but exclusive staining of cells within the synovial lining. Also normal chondrocytes and bone marrow cells showed oceasioniil staining.In biopsies from inflamed joints obtained from rats suffering from AA or CIA. an intense staining with ML 30 was seen with in the Ciirtilage-pannus junction as well as sites of bone erosion. An increased staining, compared with the normal, was also seen in chondroeytes of the eroded cartilage and in some bone marrow cells. No staining with ML 30 was seen in biopsies from inflammatory lesions due to delayed type hyperscnsitivity reactions in the skin of rats. Reactivity of ML 30 was also seen in a Western blot assay performed on lysates from inflamed synovia from rats with CIA, preferentially with a component slightly below 60 kDa in molecular weight.The demonstration of epitopes cross-reactive with hsp 65 of mycobacteria in normal and. in higher quantity, in arthritic rat joints, suggests, together with our preliminary biochemical findings, that a recently identified mammalian counterpart to bacterial hsp 65 is both preferentially expressed in normal joints and subject to increased expression in arthritis of different aetiologies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1991.tb03749.x
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  • 5
    Electronic Resource
    Electronic Resource
    Flow effects in 84Kr induced collisions in emulsion at 0.95 GeV per nucleon (1999)
    Springer
    The European physical journal 6 (1999), S. 427-430 
    Details
    ISSN: 1434-601X
    Keywords: PACS:25.70.Mn Projectile and target fragmentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract. Using emulsion detector the collective flow signals in inelastic interactions of 84Kr nuclei with Ag(Br) at 950 MeV/nucleon are studied. A transverse momentum analysis is performed to determine the reaction plane. The bounce-off of spectator fragments is observed. In azimuthal distributions relative to the reaction plane squeeze-out and side-splash of participants are seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100500050366
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  • 6
    Electronic Resource
    Electronic Resource
    Abdominopelvic actinomycosis associated with intrauterine devices. Two case reports (1988)
    Springer
    Archives of gynecology and obstetrics 243 (1988), S. 237-241 
    Details
    ISSN: 1432-0711
    Keywords: Intrauterine devices (IUD) ; Actinomycosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two case reports of abdominopelvic actinomycosis associated with an intrauterine device (IUD) are presented. In the first case, the association was difficult to establish and in the second one, a pelvic malignancy was suspected. The diagnosis and treatment of IUD-associated abdominopelvic actinomycosis are discussed on the basis of the present cases and the literature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932275
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  • 7
    Electronic Resource
    Electronic Resource
    Tumor localization with18F-2-fluoro-2-deoxy-D-glucose: Comparative autoradiography, glucose 6-phosphatase histochemistry, and histology of renally implanted sarcoma of the rat (1985)
    Springer
    Research in experimental medicine 185 (1985), S. 87-94 
    Details
    ISSN: 1433-8580
    Keywords: Glucose metabolism ; Malignant tumors ; Glucose 6-phosphatase ; Autoradiography ; Enzyme histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Rous sarcoma cells were implanted into the kidney of rats. After 5 days of growth the renal tumor was used for comparing histology with glucose 6-phosphatase (G6Pase) enzyme histochemistry (EHC) and18F-fluoro-2-deoxyglucose (FDG) auroradiography (ARG). It was found that the regions of the kidney tumor that had retained normal kidney structures were devoid of FDG, whereas there was histochemical staining of normal cortical areas. Regions of tumor growth, on the other hand, retained FDG and lacked G6Pase. Necrotic areas did not accumulate FDG. There was a dramatic decrease in the areas of G6Pase activity as a result of tumor infiltration in the kidney. The results show that FDG, currently being evaluated as a tumor detecting radiopharmaceutical indeed accumulates into areas of vital malignant growth, and they indicate that FDG positron emission tomographic (PET) images reveal the true anatomic location of malignant tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01854893
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  • 8
    Electronic Resource
    Electronic Resource
    Early detection of cell damage by supravital acridine orange staining (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 265-266 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell damage can be detected in living cells by acridine orange fluorescence earlier than with phase contrast microscopy or with conventional histological methods. The change in the acridine orange fluorescence from green to red indicates that the secondary structure of the DNA is altered very early during the cell death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02124103
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  • 9
    Electronic Resource
    Electronic Resource
    Binding of fluorescein isothiocyanate conjugated lectins to rat spermatogenic cells in tissue sections (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 575-579 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testes from three months old Spague-Dawley rats were fixed in Bouin's fluid or neutral buffered 10% formalin, embedded in paraffin, sectioned and after deparaffination stained with the following fluorescein isothiocyanate coupled lectins: PNA, WGA, Con A, RCA, SBA, DBA and UEA. The results show that there are considerable differences in the staining pattern of various spermatogenic cells between different lectins. The fixation in Bouin's fluid enhanced the staining of all the lectins compared to formalin fixation in which only a weak staining could be seen in the acrosomes of spermatids after WGA or PNA staining. PNA and WGA stained specifically the acrosome of the developing spermatids, which was seen from the beginning of the acrosome formation and lasted up to late spermiogenesis. However, the staining with PNA decreased in the late spermatids whereas the intensity of the staining remained unchanged with WGA. Con A did not stain the acrosome but stained unspecifically the cytoplasm of all spermatogenic cells. RCA stained faintly the acrosome throughout the spermatid differentiation. DBA and UEA stained specifically the chromosomes of B spermatogonia. DBA also faintly stained the cell membranes of early spermatids. SBA did not show any specific staining of the spermatogenic cells. Based on this it is suggested that the carbohydrates and glycoproteins which are known to be present in the acrosome are formed already in the beginning of the acrosome formation. The decrease in the PNA staining in late spermatids possibly reflects the fact that the receptor molecules are not synthesized in late spermatids but are formed in earlier developmental stages and are thereafter preserved in the acrosome. The enhancement of lectin binding caused by Bouin's fixative might also be applied to other tissues where previous experiments with formalin fixed tissue have failed to show any staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02400975
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  • 10
    Electronic Resource
    Electronic Resource
    Binding of fluorescein isothiocyanate conjugated lectins to rat spermatogenic cells in tissue sections (1984)
    Springer
    Histochemistry and cell biology 80 (1984), S. 475-579 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The testes from three months old Spague-Dawley rats were fixed in Bouin's fluid or neutral buffered 10% formalin, embedded in paraffin, sectioned and after deparaffination stained with the following fluorescein isothiocyanate coupled lectins: PNA, WGA, Con A, RCA, SBA, DBA and UEA. The results show that there are considerable differences in the staining pattern of various spermatogenic cells between different lectins. The fixation in Bouin's fluid enhanced the staining of all the lectins compared to formalin fixation in which only a weak staining could be seen in the acrosomes of spermatids after WGA or PNA staining. PNA and WGA stained specifically the acrosome of the developing spermatids, which was seen from the beginning of the acrosome formation and lasted up to late spermiogenesis. However, the staining with PNA decreased in the late spermatids whereas the intensity of the staining remained unchanged with WGA. Con A did not stain the acrosome but stained unspecifically the cytoplasm of all spermatogenic cells. RCA stained faintly the acrosome throughout the spermatid differentiation. DBA and UEA stained specifically the chromosomes of B spermatogonia. DBA also faintly stained the cell membranes of early spermatids. SBA did not show any specific staining of the spermatogenic cells. Based on this it is suggested that the carbohydrates and glycoproteins which are known to be present in the acrosome are formed already in the beginning of the acrosome formation. The decrease in the PNA staining in late spermatids possibly reflects the fact that the receptor molecules are not synthesized in late spermatids but are formed in earlier developmental stages and are thereafter preserved in the acrosome. The enhancement of lectin binding caused by Bouin's fixative might also be applied to other tissues where previous experiments with formalin fixed tissue have failed to show any staining.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00553720
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