ISSN:
1573-6784
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary We describe a qualitative method to predict the pre-equilibration aw, system value in which, covalent immobilized lipase B from Candida antarctica to sepharose and silica, displayed best synthetic activity. The methodology is based in the analysis of the water adsorption isotherms of the biocatalyst in air and in the organic solvent. The biocatalyst is active at pre-equilibration aw values higher than the divergence point between both isotherms. In addition, native and immobilized lipase display highest activity if the biocatalyst is pre-equilibrated at aw=P point. For preparative purposes, the validity of the method was proved in the esterification of racemic 2-(4′-isobutyl phenyl) propionic acid with 1-propanol in isooctane at long reaction time.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00184026
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