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  • 1
    Electronic Resource
    Electronic Resource
    Protein loss quantification of abraded virgin and abraded bleached hair according to Bradford assay (2005)
    Oxford, UK : Blackwell Science Ltd
    International journal of cosmetic science 27 (2005), S. 0 
    Details
    ISSN: 1468-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study intends to present Bradford assay as an alternative to Lowry test to quantify hair damage during combing or brushing. The protocol involves collecting hair fragments that are chipped away from hair during these abrasive treatments and quantitatively measuring the amount of protein using an analytical procedure to detect low amounts of proteins. This protein determination method involves the binding of Coomassie Brilliant Blue G-250 to hair protein (keratin). It is quite rapid and sensitive and less prone to interferences as the standard Lowry procedure. The latter is subject to interference from compounds such as lipids, cationic surfactants and EDTA, which are ingredients commonly used in hair care formulations and may lead to a false positive result. These drawbacks should be eliminated when using the so called Bradford method for hair protein quantitation. Our studies showed reproducible results for human hair protein and the developed color was stable for up to one hour. The data also show that virgin hair releases less protein than bleached hair. The amount detected for the former after combing ranges from 0.875 to 1.03 mg g–1 of hair and 4.85 to 5.35 mg g–1 of hair for the latter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1467-2494.2005.00257_14.x
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  • 2
    Electronic Resource
    Electronic Resource
    Immune Responses during Human Schistosomiasis Mansoni (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 24 (1986), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Splenocytes from 25 patients with severe hepatosplenic schistosomiasis mansoni were obtained after therapeutic splenectomy, Spleen cells were phenotyped and analysed for responsiveness to mitogens or heterogeneous schistosome-derived antigenic preparations (eggs. SEA; adult worms, SWAP; cercariae. CERC) in blastogenesis assays and lymphokine production systems, and were compared with peripheral blood mononuclear cells (PBMN). Splenic lymphocytes were 55% T lymphocytes (sheep erythrocyte rosette-positive) and 37% surface immunoglobulin-positive B lymphocytes. The mean T4+:T8+ ratio of these splenocytes was 1.0. Phytohaemagglutinin stimulated spleen cell production of the lymphokine mitogenic factor, but exposure to SEA or SWAP did not. Spleen cell and PBMN blastogenic responses to SEA and SWAP were sometimes, but not always in accord. Removal of plastic adherent cells allowed the non-adherent spleen cells of 30–40% of the patients to respond substantially more vigorously to SEA, SWAP and CERC. Spleen cells from a subgroup or 20–30% of the patients failed to respond to the schistosomal antigens regardless of removal of adherent cells. Spleen cell responses to gram-negative lipopolysaccharide peaked on day 5 or 6 of culture, and were augmented by adherent cell removal. Pokeweed mitogen-stimulaled responses were optimal on day 5 of culture. Spleen cells from most severe, hepatosplenic schistosomiasis mansoni patients do not respond well to schistosomal antigens or B-cell mitogens. The splenic responses of many of these patients were elevated by the removal of adherent spleen cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1986.tb02129.x
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  • 3
    Electronic Resource
    Electronic Resource
    Immune Responses during Human Schistosomiasis Mansoni (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 17 (1983), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: T lymphocyte immunoregulatory subsets (T4+ and T8+ cells) have been analysed in eight cases of hepatosplenic schistosomiasis mansoni, and compared to parallel analyses of seven intestinal Schistosoma mansoni-infected patients and four uninfected control subjects. The mean T4+:TK+ ratio in hepatosplenic cases was markedly less than observed in other groups (0.94 vs 1.86 vs 2.22. respectively). Proliferative responses stimulated by a soluble adult worm extract correlated directly with the observed T4:T8 ratios. Normal levels of E rosette-positive and surface immunoglobulin-bearing lymphoid cells were observed in intestinal patients, while hepatosplenic patients demonstrated normal B lymphocyte proportions but highly erratic E rosette-positive percentages. Curiously, in hepatosplenic patients the percentage of E rosette-positive cells often did not correlate with the proportion of T3+ cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1983.tb00793.x
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  • 4
    Electronic Resource
    Electronic Resource
    Modelling growth in cultures of Oreochromis niloticus (L.) and Cyprinus carpio L. in Pernambuco, Brazil (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Different growth models in cultures of Oreochromis niloticus (L.) and Cyprinus carpio L. were compared in Pernambuco, Brazil. The Chapman–Richards growth model was shown to be the most appropriate for Oreochromis niloticus, while Silva's model best expressed growth for Cyprinus carpio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2109.1997.00849.x
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  • 5
    Electronic Resource
    Electronic Resource
    Structure of 1,4-diethyl-3,5-dimethoxy-1,4-dihydrobenzoic acid (1993)
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 49 (1993), S. 1404-1406 
    Details
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108270193000241
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  • 6
    Electronic Resource
    Electronic Resource
    The inclusion of rhodium(II)α-methyl-cinnamate in β-cyclodextrin (1995)
    Springer
    Journal of inclusion phenomena and macrocyclic chemistry 22 (1995), S. 91-98 
    Details
    ISSN: 1573-1111
    Keywords: Rhodium(II)carboxylates ; β-cyclodextrin inclusion compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The preparation and characterization of the 1:1 inclusion compound of rhodium(II) α-methyl cinnamate in β-cyclodextrin is reported. Evidence of inclusion was obtained from X-ray powder diffraction results, Raman, IR and UV-Vis spectroscopic studies and thermal analysis. Given the potential antitumor activity of the rhodium(II) carboxylate and its virtual insolubility in water, its inclusion in β-cyclodextrin opens the possibility for its transference to the aqueous phase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00707684
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