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  • 1
    Electronic Resource
    Electronic Resource
    Genetic Strategies for Enhancing Phytoremediation (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 721 (1994), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1994.tb47418.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gene Transfer into Loblolly Pine by Agrobacterium tumefaciens (1986)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 4 (1986), S. 647-649 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have demonstrated expression of bacterial genes transferred into cells of loblolly pine (Pinus taeda L.) by Agrobacterium tumefaciens. Whereas previous surveys found pines resistant to Agrobacterium, we found two wild type strains that produce galls. Callus proliferated from these galls ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0786-647
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of genotype on in vitro adventitious shoot formation in Pinus radiata and correlations between pairs of phenotypic traits during in vitro shoot development (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 185-194 
    Details
    ISSN: 1573-5044
    Keywords: clone ; early selection ; family ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of genotype at the family and clone levels on adventitious shoot formation in Pinus radiata was examined using the cotyledon tissue culture system. Twenty-nine full-sib and two selfed families were used. Family and clone influenced all in vitro traits assessed and final adventitious shoot formation significantly. Five families selected for the long-internode trait and two selfed families used in the study were of average performance when compared to all families. Correlations using family means or clone-within-family means showed that shoot production could be predicted based on measures of early culture health. Shoot production after 16 weeks was positively related to early embryo (6 days) and early culture (4 weeks) health. Shoot production could be improved by approximately 100% through family selection, but only limited additional improvements were obtained through early embryo selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035969
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated DNA transfer in sugar pine (1990)
    Springer
    Plant molecular biology 15 (1990), S. 1-9 
    Details
    ISSN: 1573-5028
    Keywords: Agrobacterium tumefaciens ; Bo542 ; DNA transfer ; gymnosperm ; NPTII ; Pinus lambertiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract DNA transfer using Agrobacterium tumefaciens has been demonstrated in sugar pine, Pinus lambertiana Dougl. Shoots derived from cytokinin-treated cotyledons formed galls after inoculation with A. tumefaciens strains containing the plasmid pTiBo542. A selectable marker, neomycin phosphotransferase II, conferring resistance to kanamycin, was transferred into sugar pine using a binary armed vector system. Callus proliferated from the galls grew without hormones and in some cases, kanamycin-resistant callus could be cultured. Southern blots provided evidence of physical transfer of T-DNA and the nptII gene. Expression of the nptII gene under control of the nos promoter was demonstrated by neomycin phosphotransferase assays. Several aspects of DNA transfer were similar to those previously observed in angiosperms transformed by A. tumefaciens. This is the first evidence for DNA transfer by Agrobacterium in this species and the first physical evidence for transfer in any pine. These results bring us closer to genetic engineering in this commercially important genus of forest trees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017719
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata (1994)
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 195-202 
    Details
    ISSN: 1573-5044
    Keywords: adventitious roots ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Significant genotypic variation at both the family and clone-within-family levels was seen for hypocotyl rooting and the rooting of adventitious shoots produced in vitro for Pinus radiata D. Don. High rooting frequencies for hypocotyls were obtained in the absence of exogenous auxin; auxin greatly stimulated the rooting of adventitious shoots. No correlation was seen between the rooting of hypocotyls and shoots; families whose hypocotyls rooted at high frequencies did not necessarily produce shoots that rooted at high frequency. No correlation was seen between adventitious shoot production and subsequent rooting at either the family or clone level. The lack of a negative correlation indicated that selecting families or clones for high levels of shoot production will not automatically select for low rooting ability, obviating a possible bottleneck for commercial propagation of Pinus radiata. Significant family by replication interaction suggests that rooting protocols could be optimized through manipulations of the rooting environment for each family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035970
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    Paper (German National Licenses)
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