ISSN:
1573-5044
Keywords:
activation energy
;
electroporation
;
glycerol
;
permeability
;
Secale cereale
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The permeability of rye leaf protoplasts to glycerol was determined using 1,3-14C glycerol and liquid scintillation spectrometry. Estimates were 1.0×10−8 m s−1 at 0°C and 4.1×10−8 m s−1 at 22 and 31°C. The activation energy for glycerol permeability was 32.8 kJ/mol. The effect of electroporation on glycerol uptake was also explored. Treatments were performed with a field strength of 100 V/cm and an exponential decay constant of 5.8 ms. At 22 °C, electroporation affected the rate and extent of glycerol permeation, causing an increase in the intercept of the glycerol uptake curve and a decrease in the slope. Electroporation had no significant effect on glycerol uptake when performed at 0°C, when the cells were electroporated at 0°C then warmed to 31 °C, or when the cells were electroporated at 22 °C then cooled to 0°C. The results at 22°C were consistent with an influx of glycerol during electroporation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005972908734
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