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  • 1
    Electronic Resource
    Electronic Resource
    Inflammatory cell mapping of the respiratory tract in fatal asthma (2005)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 35 (2005), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The site and distribution of inflammation in the airways of asthmatic patients has been largely investigated. Inflammatory cells are distributed in both large and small airways in asthma. It has been demonstrated that distal lung inflammation in asthma may significantly contribute to the pathophysiology of the disease. The upper airways have also been implicated in the overall asthmatic inflammation. Although it is now accepted that lung inflammation is not restricted to the intrapulmonary airways in asthma, little is known about cell distribution in the other lung compartments and their relation to the intrapulmonary airways.Objective We aimed to map the inflammatory process in fatal asthma (FA), from the upper airways to the lung parenchyma.Methods Eosinophil, neutrophil, mast cell and lymphocyte content were determined in nasal mucosa, the trachea, intrapulmonary airways and parenchyma (peribronchiolar and distal) of 20 patients with FA and 10 controls.Results Eosinophil content was higher in all studied areas in FA compared with controls (P〈0.02). Mast cell content was higher in the outer area of larger airways, small membranous bronchioles and in peribronchiolar parenchyma of FA compared with controls (P〈0.04). CD3+, CD4+and CD20+cells showed increased content in FA intrapulmonary airways compared with controls (P〈0.05). There was a positive correlation between CD4+cell content in nasal mucosa and larger airways in asthmatics. Increased neutrophil content was observed only in peribronchiolar parenchyma of FA (P=0.028).Conclusion Eosinophils present a widespread distribution within the respiratory tract in FA, from the nasal mucosa to the distal lung. The outer wall of small membranous bronchioles is the main site of inflammatory changes in FA. There is a localized distribution of alveolar inflammation at the peribronchiolar region for mast cells and neutrophils. Our findings provide further evidence of the importance of the lung periphery in the pathophysiology of FA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.2005.02235.x
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  • 2
    Electronic Resource
    Electronic Resource
    Vesicles carrying nuclear material in matureCyprinus carpio erythrocytes (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 531-532 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Chromatinhaltige feulgenpositive Bläschen wurden im Cytoplasma der Erythrocyten vonCyprinus carpio gefunden. Sie haben ihren Ursprung in den unmittelbar an der Kernmembran anliegenden Mitochondrien, die während der Chromatinaufnahme allmählich ihre Struktur verlieren.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01932440
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrogen fixation capacity and nodule occupancy by Bradyrhizobium japonicum and B. elkanii strains (1998)
    Springer
    Biology and fertility of soils 27 (1998), S. 393-399 
    Details
    ISSN: 1432-0789
    Keywords: Key words Bradyrhizobium japonicum ; Bradyrhizobium elkanii ; Competitiveness ; Nitrogen fixation ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  In a previous study soybean Bradyrhizobium strains, used in Brazilian studies and inoculants over the last 30 years, and strains adapted to the Brazilian Cerrados, a region frequently submitted to environmental and nutritional stresses, were analyzed for 32 morphological and physiological parameters in vivo and in vitro. A cluster analysis allowed the subdivision of these strains into species Bradyrhizobium japonicum, Bradyrhizobium elkanii and a mixed genotype. In this study, the bacteria were analyzed for nodulation, N2 fixation capacity, nodule occupancy and the ability to increase yield. The goal was to find a relationship between the strain groups and the symbiotic performance. Two strains of Brazilian B. japonicum showed higher rates of N2 fixation and nodule efficiency (mg of N mg–1 of nodules) under axenic conditions. These strains also showed greater yield increases in field experiments when compared to B. elkanii strains. However, no differences were detected between B. japonicum and B. elkanii strains when comparing nodule occupancy capacity. The adapted strains belonging to the serogroup B. elkanii SEMIA 566, most clustered in a mixed genotype, were more competitive than the parental strain, and some showed a higher capacity of N2 fixation. Some of the adapted strains, such as S-370 and S-372, have shown similar N2 fixation rates and nodulation competitiveness to two Brazilian strains of B. japonicum. This similarity demonstrates the possibility of enhancing N2 fixing ability, after local adaptation, even within B. elkanii species. Differences in the DNA profiles were also detected between the parental SEMIA 566 and the adapted strains by analyses with the ERIC and REP-PCR techniques. Consequently, genetic, morphological and physiological changes can be a result of adaptation of rhizobia to the soil. This variability can be used to select strains capable of increasing the contribution of N2 fixation to soybean nutrition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050449
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  • 4
    Electronic Resource
    Electronic Resource
    Genome diversity in temperate bacteriophages of Oenococcus oeni (1998)
    Springer
    Archives of virology 143 (1998), S. 523-536 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  The genome structure of six bacteriophages of Oenococcus oeni was compared. Two distinct groups with no apparent restriction site conservation were defined. In members of the α group (fOgML34, fOg4029, fOg30 and fOg218) a 7.5 kb region containing the origin of DNA packaging (cos) was highly conserved. Stretches of DNA heterogeneity could also be assigned to particular regions and were mostly evident in the right area of the genomes. fOg44 and fOgPSU1 (β group) were indistinguishable in the left half of their genomes, including cos, but were markedly dissimilar in other regions. Strong labelling signals detected in cross-hybridizations involving members of different groups were confined to fragments centrally located in their physical maps. The attachment site (attP) of fOg44 was assigned to this conserved region. It is suggested that recombination events at this location may have been important in generating the observed diversity of oenophage genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050308
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  • 5
    Electronic Resource
    Electronic Resource
    Physical variables in gauge theories (1992)
    Springer
    The European physical journal 55 (1992), S. 271-274 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The symplectic method is applied to obtain the physical variables and the physical Hamiltonian in two examples of gauge theories: the electrodynamics in the Coulomb gauge and the two-dimensional bosonic Schwinger model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01482589
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  • 6
    Electronic Resource
    Electronic Resource
    Embryogenesis induction in petals of Araujia sericifera (1997)
    Springer
    Plant cell, tissue and organ culture 51 (1997), S. 95-102 
    Details
    ISSN: 1573-5044
    Keywords: benzyladenine ; light ; petals ; silver thiosulfate ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The embryogenic capacity of Araujia sericifera petals and some of the factors involved in the induction of embryos was investigated. The influence of 6-benzyladenine and α-naphthalene acetic acid, light intensity (90 or 5 µmol m-2 s-1) and silver thiosulphate (inhibitor of ethylene action) were studied. It was found that petals are an easy system in which to induce somatic embryogenesis. Plants were recovered from somatic embryos. Although 6-benzyladenine is essential for inducing an efficient response, a high dosage increased callogenesis and reduced embryogenesis. The highest rate of embryogenesis is induced with high light intensity (90–100 µmol m-2 s-1), even though the presence of silver thiosulphate in the medium markedly reduced embryo induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005910929421
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of some exogenous amino acids on the production of maize embryogenic callus and on endogenous amino acid content (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 1-11 
    Details
    ISSN: 1573-5044
    Keywords: embryogenic Type 1 callus ; free amino acids ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of four exogenous amino acids (proline, glycine, asparagine and serine) on the production of maize embryogenic callus and on its endogenous amino acid content have been investigated. For this purpose, an established embryogenic line of Type 1 callus from the inbred W64Ao2 has been used. From the results it may be concluded that a concentration of proline exceeding 6 mM is negative for the production of embryogenic callus. When proline is eliminated from the medium, other amino acids tested in certain concentrations yield a percentage of embryogenic callus production that exceeds or equals that of proline. The endogenous free proline content in embryogenic callus is significantly higher than that in non-embryogenic callus regardless of proline presence in the medium. The only exception are the glycine-containing media, in which endogenous free alanine of embryogenic callus increases at the expense of endogenous free proline. This study suggest a positive role of endogenous free proline or alanine accumulation in the embryogenic callus production which might be related to an adaptation to the metabolic changes produced by in vitro culture and embryogenesis induction. Furthermore, these results indicate that treatments with amino acids that are different from proline can be used to improve the efficiency of embryogenic callus production from well established maize callus cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048457
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  • 8
    Electronic Resource
    Electronic Resource
    Anisotropic voter model (1995)
    Springer
    Journal of statistical physics 78 (1995), S. 963-970 
    Details
    ISSN: 1572-9613
    Keywords: Stochastic spin systems ; majority vote models ; anisotropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A majority vote model subject to anisotropic voting rules is studied in two dimensions using a first-order mean-field approximation and Monte Carlo simulations. The critical behavior is consistent with the 2D Ising universality class.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02183696
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  • 9
    Electronic Resource
    Electronic Resource
    Influence of exogenous proline on embryogenic and organogenic maize callus subjected to salt stress (1996)
    Springer
    Plant cell, tissue and organ culture 47 (1996), S. 59-65 
    Details
    ISSN: 1573-5044
    Keywords: free amino acids ; organogenic callus ; polyamines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of exogenous proline (6 mM) and increasing NaCl doses (from 0.4 to 1.2% w/v) on the maintenance of organogenic and embryogenic callus lines derived from the salt-sensitive maize inbred W64Ao2 were studied. To this end, total protein, free amino acid and polyamine content were analyzed. The demand of exogenous nitrogen and especially of proline, even in the presence of salt, differed in the two types of morphogenic calluses. The total protein content of embryogenic calluses was higher in the presence of proline than in its absence, in all the cases studied. An opposite effect of proline was observed in organogenic calluses: the presence of proline and salt decreased significantly their protein content. With respect to amino acid and polyamine contents, the organogenic calluses showed physiological characteristics of salt-adaptation, whereas the embryogenic calluses were more sensitive to NaCl. Although endogenous proline increased in the organogenic calluses cultured in the presence of salt, in embryogenic calluses it only rose at the lowest salt concentration. Furthermore, the endogenous arginine content under saline conditions was higher in organogenic calluses. A compensatory effect between proline and polyamine metabolism related to the endogenous arginine content in response to salt stress was also observed. This effect differed in the two types of calluses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02318966
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in Araujia sericifera (1992)
    Springer
    Plant cell, tissue and organ culture 29 (1992), S. 269-274 
    Details
    ISSN: 1573-5044
    Keywords: cotyledons ; friable calluses ; somatic embryos ; cell suspensions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This work presents the preliminary results of in vitro studies with Araujia sericifera, which is cultivated for ornamental purposes. Immature seeds from wild plants were used to start the cultures. Somatic embryos and friable embryogenic calluses were obtained from white cotyledons in media containing naphthaleneacetic acid and benzyladenine or 2,4-dichlorophenoxyacetic acid. Plants were regenerated from these somatic embryos. Cell suspensions obtained from friable calluses cultured in M1 modified medium showed a considerable growth capacity. The packed cell volume was doubled in about 15 days of culture at the exponential phase. the results obtained may be used to design further experiments with the aim of improving somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034362
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