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  • 1
    Electronic Resource
    Electronic Resource
    A cdc2 gene of Petunia hybrida is differentially expressed in leaves, protoplasts and during various cell cycle phases (1992)
    Springer
    Plant molecular biology 20 (1992), S. 1121-1130 
    Details
    ISSN: 1573-5028
    Keywords: cdc2 gene expression ; cell cycle ; Petunia ; protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of p34cdc2 kinase in higher eukaryotes has demonstrated that p34cdc2 function is conserved in all eukaryotic cells. The p34cdc2 kinase (the product of the cdc2 gene) is required during the G1 cell cycle phase at the initiation of DNA replication and also in G2-M phases for entry into mitosis. In this paper we report the isolation and characterization of a cdc2 Petunia hybrida PCR fragment (cdc2Pet). Using a DNA probe based on this fragment and a p34cdc2-specific antibody, cdc2Pet transcript and p34 protein levels were found to be constant both in 2C nuclei of highly proliferating mesophyll 2C cells derived from protoplasts and in 2C nuclei isolated directly from expanded petunia leaves. Both the cdc2Pet transcript and p34cdc2 protein levels were found to be higher in nuclei at 4C than in those at 2C, even when these 4C nuclei were from non-proliferating tissue. Thus cdc2Pet mRNA and protein levels measured in different tissues should not be interpreted to reflect exclusively the proliferative state of the tissue but also the frequency of G2 cells including those in the differentiated state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028898
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Relation between protoplast division, cell-cycle stage and nuclear chromatin structure (1988)
    Springer
    Protoplasma 142 (1988), S. 127-136 
    Details
    ISSN: 1615-6102
    Keywords: Cell cycle ; Protoplast division ; Chromatin structure ; Flow-cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using different sources of protoplasts and two complementary techniques, flow cytometry and image analysis, to study the cell-cycle phases, we sought to define the particular protoplast state associated with the disposition to divide. Both inPetunia and inNicotiana plumbaginifolia, tissues with a higher G2 frequency (from different aged plants) yielded protoplasts capable of increased cell division. InSorghum, the age of the plant does not modify the proportion of G2 nuclei in leaf protoplasts, and we used root protoplasts to increase G2 frequencies. InHelianthus annuus, leaf protoplasts did not divide; however, hypocotyl protoplast preparations with relatively high 4C DNA frequencies do divide. Moreover, image analysis of chromatin structure indicated that leaf nuclei were in the G0 phase, unlike those from hypocotyls which were in G1. A high frequency of protoplasts with G2 nuclei appears to be correlated with the ability of a given preparation to undergo division; conversely, the differentiated G0 state is not conducive to division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01290869
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    Paper (German National Licenses)
    Fulltext
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