ISSN:
0091-7419
Keywords:
microbial mannans
;
surface antigens
;
mannosidosis
;
concanavalin A
;
pea lectin
;
sperm
;
blastocyst
;
Life Sciences
;
Molecular Cell Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Rabbit antibodies to cell wall mannans of various microbial strains and their mutants were found to be cross-reactive to cell carbohydrates of mammalian sperm and 4-6-day-old blastocysts. Immunochemical studies indicate that oligomers of α1→2, α1→3, α1→6, and probably also α→4 linked mannose residues of sperm carbohydrates are available for antibody binding. At least 80% of binding activity of a yeast mannan antibody to sperm can be effectively inhibited by specific haptens or digestion with exo-α-D-mannosidase, an enzyme activity highest in testicular tissue. In order to determine the role of this enzyme in the metabolism of the cross-reactive mannan antigens of sperm, the relative amount of a specific α-linked oligomannosyl determinant of bovine sperm from homozygous normals was compared to that of heterozygous carriers of α-mannosidase deficiency.Extensive cross-reactivity between the microbial and mammalian oligomannosyl determinants suggest that these are conserved structures in cell carbohydrates, although the organization of these units in the microbial cell wall lipopolysaccharide has very little similarity to the carbohydrate moieties of mammalian glycoproteins.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jss.400060414
Permalink
