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Genetic control of in vitro-organogenesis in recombinant inbred lines of sunflower (Helianthus annuus L.) (1999)

Berrios, E. F. ; Gentzbittel, L. ; Alibert, G. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Plant breeding 118 (1999), S. 0

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ISSN: 1439-0523

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Ninety-three recombinant inbred lines (F8) of sunflower developed by the single-seed descent method from the cross ‘PAC-2 × RHA-266’ were used to screen their regenerability by organogenesis. The experiment was designed in randomized complete blocks with three replications. Each replication consisted of 10 Petri dishes with four explants. Cotyledons were excised from 2-day-old seedlings. Each cotyledon was divided into two pieces (four explants), which were incubated in solid regeneration medium consisting of full-strength Murashige and Skoog medium modified by adding hormones. A high genotypic variability for organogenesis parameters between genotypes was observed in this study. The difference between all recombinant F8 lines and their parents was not significant, showing that the 93 inbred lines used in this experiment are representative of the total possible recombinant lines from the cross ‘PAC-2 × RHA-266′. These F8 lines were not consciously selected for any trait; therefore, they represent a random set of lines segregating for the organogenesis parameters, as well as for other traits which could be important for breeding.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1046/j.1439-0523.1999.00370.x

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Early and late platelet sequestration in different organs during endotoxic shock (1988)

Al-Sarraf, A. A. ; Christenson, J. T. ; Owunwanne, A.

Springer

Research in experimental medicine 188 (1988), S. 59-66

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ISSN: 1433-8580

Keywords: Endotoxic shock ; Platelet sequestration ; Indium-111-oxine platelets ; Adult respiratory distress syndrome

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Using a sheep model, platelet sequestration in lungs, liver, kidney, spleen, and brain was studied after the injection of Indium-111-oxine-labeled platelets andE. coli lipopolysaccharide. Immediately following endotoxin-induced shock, platelet sequestration was observed in lungs, liver, and kidney with corresponding decrease in measured Indiun-111-oxine platelets in spleen and circulating blood. There was also a decrease in platelet counts as measured in a phase contrast microscope. The early platelet sequestration in lung, liver, and kidney was followed by platelet disaggregation and another phase of platelet sequestration which continued until the death of the animal. No platelet sequestration was observed in the brain.