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  • 1
    Electronic Resource
    Electronic Resource
    Graft size-matching in living related partial liver transplantation in relation to tissue oxygenation and metabolic capacity (1996)
    Springer
    Transplant international 9 (1996), S. 15-22 
    Details
    ISSN: 1432-2277
    Keywords: Liver transplantation, living related ; Living related liver transplantation ; Segmental liver transplantation ; Tissue oxygenation, liver transplantation ; Metabolic capacity, liver transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of graft size-matching on tissue oxygenation and metabolic capability was studied in living related partial liver transplantations for 47 pediatric patients. Their age ranged from 4 months to 17 years 3 months, their body weight from 4.0 to 58.0 kg, graft weight from 191 to 440 g, and graft weight/recipient body weight ratio from 0.61% to 6.0%. Tissue oxygenation and its heterogeneity were investigated by measuring oxygen saturation of hemoglobin in the liver sinusoid (SO2), coefficient of variation of SO2, and arterial ketone body ratio. The metabolic capacity of the graft was investigated by measuring bilirubin clearance, recovery of cholesterol esterification, and ketone body production. In infants with a relatively large liver graft, both intra- and extracellular oxygenation remained low soon after reperfusion but recovered to the control value by the end of the operation. In adolescent recipients of a relatively small graft, by contrast, synthetic and detoxification capacities were relatively deficient; however, these improved with time. These results indicate that sufficient tissue oxygenation and liver regeneration are essential for successful liver transplantation with relatively large and small grafts, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336807
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  • 2
    Electronic Resource
    Electronic Resource
    Testicular volume in Japanese boys up to the age of 15 years (2000)
    Springer
    European journal of pediatrics 159 (2000), S. 843-845 
    Details
    ISSN: 1432-1076
    Keywords: Key words Prader orchidometer ; Testicular size ; Cross-sectional data ; Puberty
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies have indicated that Japanese children grow and mature significantly faster than Caucasian children, thus calling for a separate reference standard for each skeletal and sexual maturity index. To establish normal reference values for testicular volume in Japanese boys, we studied from 1985 to 1995, 900 healthy male children of 0 to 15 years of age for medical history, physical examination, height, weight, sitting height, and head circumference measurements, Tanner sex maturity stage, and testicular size. The testicular volume was determined using a Prader orchidometer by the same observer (N.M.). Based on these data, we established the cross-sectional percentile growth curves (90th, 50th, 10th percentiles) for testicular volume of Japanese boys. The testicular volume of 3 ml was attained at 9.3 years of age (90th percentile), 11.0 years of age (50th percentile), and 12.1 years of age (10th percentile), respectively. Conclusion Swelling of the testis in Japanese children begins approximately 1 year earlier than in Swiss children in accordance with the earlier skeletal maturation in Japanese children.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008350
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  • 3
    Electronic Resource
    Electronic Resource
    Occurrence of pppApp-synthesizing activity in actinomycetes and isolation of purine nucleotide pyrophosphotransferase (1976)
    Springer
    Archives of microbiology 107 (1976), S. 183-187 
    Details
    ISSN: 1432-072X
    Keywords: pppApp-synthesizing activity ; ppGpp and pppGpp-purine nucleotide pyrophosphotransferase ; Actinomycetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of adenosine 5′-triphosphate-3′-diphosphate-synthesizing activity was detected in five strains of actinomycetes; Streptomyces morookaensis, Streptomyces aspergilloides, Streptomyces hachijoensis, Actinomyces violascens and Streptoverticillium septatum, out of 825 strains of actinomycetes, bacteria, fungi and imperfecti. Purine nucleotide pyrophosphotransferase were extracellularly excreted associating with the cell growth, and were purified partially or to apparent homogeniety from the culture filtrate. The enzymes are a monomeric protein with a molecular weight of 18000–26000 and synthesize adenosine, guanosine and inosine 5′-phosphate (mono, di or tri)-3′-diphosphate such as pApp, ppApp, pppApp, pGpp, ppGpp, pppGpp and pppIpp by transferring a pyrophosphoryl group from the 5′-position of ATP, dATP and pppApp to the 3′-position of purine nucleotides in the presence of a divalent cation and in alkaline state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446837
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  • 4
    Electronic Resource
    Electronic Resource
    A case of linitis plastica of the rectum treated by pelvic exenteration after aggressive immunochemotherapy (1992)
    Springer
    Surgery today 22 (1992), S. 159-162 
    Details
    ISSN: 1436-2813
    Keywords: linitis plastica ; total pelvic exenteration ; intraarterial infusion ; OK-432
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Multidisciplinary treatment was administered to a 32 year-old man with primary linitis plastica of the rectum, which was considered inoperable due to an extensive local spread at the first operation. Thirty KE of OK-432 was injected into the tumor per week (total 90-KE) and 5 KE of OK-432 was inoculated into the intracutaneous space per week (total 125 KE). Also methotrexete (MTX 50 mg) and 5-FU (500 mg) combined were given each week by intra-arterial infusion (total 20 courses). After this immuno-chemotherapy the CEA decreased from the pretreatment value of 12.7 ng/ml to 3.6 ng/ml and the tumor size was reduced. A total pelvic exenteration could then be performed almost curatively. As a result, this patient was able to return to the society with a better quality of life and has survived for 20 months since presentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00311342
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  • 5
    Electronic Resource
    Electronic Resource
    Hiatal herniation of the colon with digestive tract bleeding (1990)
    Springer
    Surgery today 20 (1990), S. 582-584 
    Details
    ISSN: 1436-2813
    Keywords: colonic herniation ; digestive tract bleeding ; sliding esophageal hernia ; Hill's gastropexy method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 71 year old woman with hiatal herniation was admitted to our hospital with anemia and melena. A barium enema revealed hiatal herniation of the transverse colon in conjunction with the stomach, which seemed to be strangulated by the esophageal hiatus. She underwent surgery, for which Hill's gastropexy method was employed and has had no signs of bleeding since. By presenting this case, we stress the importance of marking a careful differential diagnosis due to the variability in symptoms of digestive tract bleeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02471016
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  • 6
    Electronic Resource
    Electronic Resource
    Animal cell cultivation for production of biological substances with a novel perfusion culture apparatus (1983)
    Springer
    Methods in cell science 8 (1983), S. 167-171 
    Details
    ISSN: 1573-0603
    Keywords: perfusion culture ; Namalva cells ; lymphoblastoid cells ; cell sedimentation column ; serum free medium ; interferon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Specific standard methods of a new perfusion culture are described for growth and maintenance of mammalian cells in suspension culture at high density. The new system of perfusion consists of a suspension vessel containing a cone-type cell-sedimentation column as cell separator and serving as well for axis of impeller. In this report, Namalva cells (human lymphoblastoid cells), adapted to a serum-free medium, have been grown in this perfusion system. The cultured cells and the conditioned medium were separated in the cell-sedimentation column; cell-free expended medium was drained from culture vessel to effluent vessel. The cell-sedimentation column device has been used for the propagationin vitro of Namalva cells to densities 7 to 10 × 106 cells/ml in serum-free medium supplemented with insulin, transferrin, sodium pyruvate, selenious acid, and galactose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665880
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  • 7
    Electronic Resource
    Electronic Resource
    Efficient expression of human beta-interferon in Namalwa KJM-1 cells adapted to serum-free medium by a dhfr gene coamplification method (1990)
    Springer
    Cytotechnology 4 (1990), S. 173-180 
    Details
    ISSN: 1573-0778
    Keywords: beta-interferon ; dihydrofolate reductase ; gene coamplification ; Namalwa KJM-1 ; serum-free medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We previously reported the expression of human beta-interferon (β-IFN) (Miyajiet al., 1989) and human lymphotoxin (Miyajiet al., 1990) in Namalwa KJM-1 cells adapted to serum-free medium. To establish an efficient gene expression system, a dihydrofolate reductase (dhfr) gene coamplification method was applied to this cell line. A β-IFN expression plasmid was introduced with a dhfr expression plasmid into KJM-1 and methotrexate (MTX)-resistant derivatives were selected by a stepwise increase of MTX concentration. Among them, derivatives which showed higher expression levels of β-IFN than that achieved by the parental transformants were obtained, suggesting that a dhfr gene coamplification method can be used for efficient expression of foreign genes in KJM-1 which contains endogenous dhfr genes. Then, an improved β-IFN expression vector was constructed, which contains a dhfr transcription unit. This plasmid was introduced into KJM-1 and then, MTX-resistant derivatives were selected. Among them, the highest producer, clone 40-10-24, secreted β-IFN at a level as high as 5 μg/ml, which is about 100-fold higher than that obtained by the G418-resistant parental transformants. In addition, β-IFN produced by recombinant KJM-1 cells had the same molecular weight of that produced by fibroblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365098
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  • 8
    Electronic Resource
    Electronic Resource
    Optimization of cell culture conditions for production of biologically active proteins (1991)
    Springer
    Cytotechnology 5 (1991), S. 17-34 
    Details
    ISSN: 1573-0778
    Keywords: genetic engineering ; Namalwa cells ; perfusion culture ; scaling-up ; serum-free medium ; stable production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We investigated the basic technology of cell culture conditions for production of useful substances such as cytokines, and related proteins produced by Namalwa cells. Namalwa cells (Klein, 1972), human B lymphoblastoid cells, were used for large scale production of alpha-interferon (Klein, 1979). Namalwa KJM-1, a subline of Namalwa cells, adapted to serum- and albumin-free medium, can grow at a high density above 1 × 107 cells/ml in suspension mode by the use of a perfusion culture system, Biofermenter™, containing a cone-type cell-sedimentation column as cell separator (Sato, 1983). Several kinds of cytokine cDNA can be introduced and expressed in Namalwa KJM-1 cells (Miyaji, 1990a,b,c). Some of these were produced in large quantities by use of a gene amplification method with dhfr (Miyaji, 1990c), even though the Namalwa KJM-1 cells contained endogenous dhfr genes. For stable production of the target protein, Namalwa KJM-1 cells are very useful host cells, because they have no effective endogenous protease activity in the conditioned medium. Using Biofermenter with micro-silicone fibers and a dialysis system, the specific productivity of the target proteins was not depressed at a high cell density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00573878
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  • 9
    Electronic Resource
    Electronic Resource
    Establishment of Namalva cell lines which grow continuously in glutamine-free medium (1988)
    Springer
    Cytotechnology 1 (1988), S. 151-158 
    Details
    ISSN: 1573-0778
    Keywords: adaptation ; ammonia ; glutamine-free ; glutamine synthetase ; Namalva cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Glutamine has been shown to be a preferred energy source for some established cell lines and cancer cells in culture (Kovacevic, 1971; Kovacevic, 1972; Lavietes, 1974). Empirically, glutamine is the most abundant amino acid in most of the culture media developed. The major end product of glutamine metabolism is ammonia. Ammonia build up is one of the limiting factors in the proliferation of mammalian cells in higher density culture and is directly related to the initial glutamine concentration. The susceptibility of glutamine to thermodecomposition prevents the heat sterilization of glutamine-enriched media and this significantly increases the cost of medium preparation at the industrial scale. In an attempt to overcome these drawbacks, a population of Namalva cells capable of growing in glutamine-free media was established. The adapted cells were found to contain a higher level of glutamine synthetase activity which enable them to synthesize sufficient amounts of glutamine for their growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00146816
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  • 10
    Electronic Resource
    Electronic Resource
    Stable production of recombinant pro-urokinase by human lymphoblastoid Namalwa KJM-1 cells: Host-cell dependency of the expressed-protein stability (1993)
    Springer
    Cytotechnology 13 (1993), S. 79-88 
    Details
    ISSN: 1573-0778
    Keywords: Chinese hamster ovary cells ; Namalwa KJM-1 cells ; protease activity ; pro-urokinase ; serum-free medium ; stability of expressed-protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Human pro-urokinase (pro-UK) gene was engineered for expression in mammalian cells. The stability of recombinant pro-UKs produced by two kinds of cells, Chinese hamster ovary (CHO) and human lymphoblastoid Namalwa KJM-1 cells, were compared. The pro-UK expressed in CHO cells in serum-free medium was degraded by cysteine endopeptidase secreted by CHO cells. This endopeptidase was inhibited by pchloromercuribenzonate (PCMB) and leupeptin more efficiently than by aprotinin. On the other hand, the pro-UK expressed in Namalwa KJM-1 cells was not degraded, resulting in the stable production of pro-UK at a rate of 2–3 μg/106 cells/day by use of a gene amplification method with dihydrofolate reductase (DHFR) in serum-free medium. Thus, Namalwa KJM-1 cells showed the desired characteristics as a host cell for the production of recombinant proteins. The stability of recombinant proteins produced in heterologous systems may vary depending on the host cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749934
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