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  • 1
    Electronic Resource
    Electronic Resource
    Observation of a porous gel structure in poly(p-phenylenebenzobisthiazole)/97% sulfuric acid (1986)
    s.l. : American Chemical Society
    Macromolecules 19 (1986), S. 2856-2859 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma00165a032
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  • 2
    Electronic Resource
    Electronic Resource
    Chemistry of micelles series. Part 2. Cascade molecules. Synthesis and characterization of a benzene[9]3-arborol (1986)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 108 (1986), S. 849-850 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00264a054
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  • 3
    Electronic Resource
    Electronic Resource
    Cascade molecules. Part 6. Synthesis and characterization of two-directional cascade molecules and formation of aqueous gels (1990)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 112 (1990), S. 8458-8465 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00179a034
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of membrane-associated calcium following cytokinin treatment in Funaria using chlorotetracycline (1981)
    Springer
    Planta 152 (1981), S. 272-281 
    Details
    ISSN: 1432-2048
    Keywords: Bryophyta ; Bud formation (moss) ; Calcium ; Chlorotetracycline ; Cytokinin ; Funaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the changes in membrane-associated calcium that occur during cytokinin induced bud formation in Funaria hygrometrica Hedw. using the fluorescent Ca2+-chelate probe chlorotetracycline (CTC). In the target caulonema cells a localization of CTC fluorescent material becomes evident at the presumptive bud site 12 h after cytokinin treatment. By the time of the initial asymmetric division this region is four times as fluorescent as the entire caulonema cell. Bright CTC fluorescence remains localized in the dividing cells of the bud. To relate the changes in CTC fluorescence to changes in Ca2+ as opposed to membrane-density changes we employed the general membrane marker N-phenyl-1-naphthylamine (NPN). NPN fluorescence increases only 1.5 times in the initial bud cell. We conclude that the relative amount of Ca2+ per quantity of membrane increases in this localized area and is maintained throughout bud formation. We suggest that these increases in membrane-associated Ca2+ indicate a localized rise in intracellular free Ca2+ concentration brought about by cytokinin action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385156
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  • 5
    Electronic Resource
    Electronic Resource
    Immunofluorescence visualization of phytochrome in Pisum sativum L. epicotyls using monoclonal antibodies (1983)
    Springer
    Planta 159 (1983), S. 545-553 
    Details
    ISSN: 1432-2048
    Keywords: Immunosorbent assay (phytochrome) ; Monoclonal antibody ; Phytochrome (immunofluorescence) ; Pisum (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have investigated the cellular distribution of phytochrome in epicotyls of dark-grown pea (Pisum sativum L.) seedlings using monoclonal antibodies to pea phytochrome. Screening of the eight available antibodies both by an enzymelinked immunosorbent assay (ELISA) and by their ability to visualize phytochrome in situ by immunocytochemical fluorescence demonstrated that: (1) three antibodies work well for immunofluorescence; (2) none of the eight antibodies discriminates between the red- and the far-red-absorbing forms of phytochrome (Pr, Pfr) as assayed by ELISA; (3) the antigenicity of phytochrome is reduced by fixation with formaldehyde with respect to all eight antibodies; and (4) two antibodies that bind well to formaldehyde-fixed phytochrome as assayed by ELISA do not bind well to phytochrome in situ. Phytochrome is observed in both cortical and stomatal guard cells of the epicotyl and exhibits a homogeneous cytoplasmic distribution in non-irradiated tissue. After red-light (R) treatment phytochrome becomes transiently inaccessible to antibodies. If maintained in the Pfr form for 10 min at room temperature before fixation, at least a portion of the phytochrome pool becomes accessible to antibodies and assumes a “sequestered” distribution. Both of these effects are almost entirely either prevented or reversed by subsequent far-red light treatment. We believe that the transient inaccessibility of phytochrome to antibodies after R irradiation is not a function of its conformational state. We suggest instead that R treatment rapidly induces an association of phytochrome with a subcellular component that interferes with antibody binding and that the “sequestered” areas represent a phytochrome pool that is distinct from both the diffusely distributed phytochrome in non-irradiated cells and from that phytochrome which is inaccessible to antibodies immediately after R irradiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409144
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  • 6
    Electronic Resource
    Electronic Resource
    Correlation of electrical current influx with nuclear position and division inFunaria caulonema tip cells (1986)
    Springer
    Protoplasma 132 (1986), S. 32-37 
    Details
    ISSN: 1615-6102
    Keywords: Ion channels ; Funaria protonema ; Nuclear positioning ; Vibrating microelectrode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ionic currents around caulonema tip cells of the filamentous protonema of the mossFunaria hygrometrica were examined using a nonintrusive vibrating microelectrode to map electrical current before and during mitosis. Tip cells in interphase generate inward electrical currents that are maximal at the nuclear region. These currents remain concentrated over the nucleus as it migrates forward maintaining a constant distance from the growing tip. Just prior to mitosis this inward current increases twofold. During mitosis and cytokinesis current at the nuclear zone increases to four times the resting level and fluctuates, falling to zero after cell plate fusion with parental walls. The locus of outward current could not be dectected. These results suggest that plasma membrane ion currents may regulate both nuclear positioning and subsequent temporal and spatial control of cell division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275787
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  • 7
    Electronic Resource
    Electronic Resource
    Distortion of plant cell plate formation by the intracellular-calcium antagonist TMB-8 (1988)
    Springer
    Protoplasma 144 (1988), S. 92-100 
    Details
    ISSN: 1615-6102
    Keywords: Calcium ; Cell plate ; Funaria ; Phragmoplast ; TMB-8
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Caulonema tip cells ofFunaria deposit new oblique cross walls of specific morphology and placement by a highly defined reorientation mechanism. In the presence of the purported intracellular Ca2+ antagonist 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8), these cross walls form in the proper place but exhibit a distorted morphology. Video microscopy indicates that the deformation takes place during the reorientation of the cell plate from a perpendicular to an oblique configuration. Electron micrographs of TMB-8 treated cells indicate a stabilization of phragmoplast microtubules and a greater amount of vesicles and membrane in the developing cell plate. TMB-8 treated cells also show intense chlortetracycline fluorescence from mitochondria, vesicles and endoplasmic reticulum as compared to untreated cells indicating that TMB-8 is blocking release of Ca2+ from intracellular stores. It is concluded that this may cause distortation of cross walls as they form by delaying vesicle fusion, stabilizing microtubules, and increasing the amount of new wall material in the developing cell plate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01637241
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