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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Addiction 89 (1994), S. 0 
    ISSN: 1360-0443
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Psychology
    Notes: To determine haw the injecting behaviors of injection drug users (IDUs) change over time in the context of the epidemic of acquired immunodeficiency syndrome (AIDS) and what factors may be associated with such changes, a cohort of IDUs (n = 313) initially in treatment provided structured interviews regarding drug injecting behaviors. Repeat interviews in 18 months assessed behavior change in subjects who could be contacted for follow-up (n = 220, 70.3%). The study occurred in a state where sterile syringes can be purchased without prescription in drug stores. Injection drug use occurred for 180 (81.8%) of the 220 subjects in the 12 months prior to the initial interview but in only 121 (55.0%) in the 10 months prior to the follow-up interview (p 〈 0.0001). Similarly, sharing of equipment decreased from 63.1% to 31.8% (p 〈 0.0001). Sharing with multiple partners declined from 41.9% to 10.6% (P 〈 0.0001). Factors associated with ongoing risk included use of injected and non-injected psychoactive substances, less time in drug dependence treatment during follow-up interval, having a sexual partner who was an IDU and not using a drug store as the primary source of syringes. Factors associated with multiple-partner sharing included use of psychoactive substances, younger age and nonwhite race.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1360-0443
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Psychology
    Notes: Predictors of methadone maintenance treatment outcome have not been extensively studied as they relate to variations in program philosophy, nor have such predictors received much examination among recently treated, older cohorts of opioid addicts for whom drug use patterns have changed. Predictors of outcome were examined at 18 months post-treatment entry for 353 admissions to methadone maintenance who received random assignment to one of three counseling conditions: (1) medication only, (2) standard counseling and (3) enhanced services; and one of two contingency conditions: (1) no contingencies, and (2) contingency contracting in a six-cell 3x2 design. Subjects in contingency contracting conditions were placed on contingency contracts for positive urine toxicology results and ultimately discharged for unremitting drug use. All subjects completed the Addiction Severity Index (ASI) and provided weekly urine specimens. Predictors of urinalysis results and treatment retention were determined using bivariate and multivariate techniques. Interactions between subject characteristics by experimental condition assignment were also examined as predictors. Higher rates of total positive urine specimens were predicted by younger age, greater pre-treatment frequency of smoking cocaine, lower ASI psychiatric composite scores, and higher ASI legal composite scores. Higher rates of opiate positive specimens were predicted by younger age, lower pre-treatment frequency of alcohol intoxication, higher ASI legal and lower ASI employment and psychiatric composite scores, and assignment to medication only/no contingencies condition. Higher rates of cocaine positives were predicted by younger age, black race, lower ASI psychiatric composite score, greater pre-treatment frequency of intravenous and smoked cocaine use, less pre-treatment frequency of marijuana use, and lower methadone dose level. Assignment to enhanced/contingency contracting predicted lower rates of cocaine positives. Treatment retention was predicted by older age, non-black race, lower ASI legal composite score, higher methadone dose level and assignment to non-contingent conditions. While subject variables over which treatment providers have little control were, thus, related to outcome, type of treatment provided and methadone dose also influenced outcome.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Human mast cells and basophils that express the high-affinity immunoglobulin E (IgE) receptor, Fcϵ receptor 1 (FcϵRI), have key roles in allergic diseases. FcϵRI cross-linking stimulates the release of allergic mediators. Mast cells and basophils co-express FcγRIIb, a low ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature medicine 11 (2005), S. 446-449 
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Animal allergens are an important cause of asthma and allergic rhinitis. We designed and tested a chimeric human-cat fusion protein composed of a truncated human IgG Fcγ1 and the major cat allergen Fel d1, as a proof of concept for a new approach to allergy immunotherapy. This Fcγ-Fel ...
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-2592
    Keywords: Pemphigus vulgaris ; immunoregulation ; monoclonal antibodies ; autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract T cells from nine patients in the active stage of pemphigus vulgaris and five in the inactive stage of the disease were studied with Leu-1, Leu-2, and Leu-3 monoclonal antibodies. No significant differences were observed in the proportions of total T cells or T cells expressing either helper or suppressor phenotype in peripheral blood leukocytes of patients compared to normal subjects. Immunoregulatory mechanisms were functionally studied using an assay measuring total IgG synthesizedin vitro. Peripheral blood leukocytes were separated into T- and B-cell fractions and cultured in various combinations. In nine experiments, the T cells were irradiated prior to culturing with B cells to remove their suppressor function. No statistically significant differences were observed in the total IgG synthesized by B cells obtained from patients and normal subjects when cultured with untreated T cells or irradiated T cells obtained from patients or normal controls. These results indicated that there was no loss of suppressor-cell function or increased helper-cell function when assessed by measuring the total IgG synthesized. The addition of serum from pemphigus patients to peripheral blood leukocyte cultures of pemphigus patients and normal controls had no statistically significant effect on the synthesis of total protein or on the amount of Ig synthesized and secreted. Peripheral blood leukocytes from six untreated patients with pemphigus vulgaris were stimulatedin vitro with pokeweed mitogen (PWM) to produce immunoglobulin. The IgG produced selectively bound to the intercellular cement substance of the epidermis of patients' perilesional skin, normal human skin, and monkey esophagus. The IgG was biosynthetically labeled by culturing the leukocytes in medium supplemented with [3H]leucine, and the binding of the radiolabeled IgG was visualized by autoradiography. The IgG nature of the protein was demonstrated by precipitation withStaphylococcus protein A and removal with rabbit anti-human IgG antisera. Peripheral blood leukocytes obtained from normal volunteers and control patients did not produce this antibody. Our studies indicate that there was no general functional or phenotypic alteration of suppressor or helper T cells in the peripheral blood. The peripheral blood leukocytes of pemphigus patients under PWM stimulation can produce an anti-intercellular cement substance antibodyin vitro. These results indicate that the abnormality of immunoregulation which resulted in the production of a pathogenetic autoantibody in pemphigus is highly specific.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of clinical immunology 5 (1985), S. 38-45 
    ISSN: 1573-2592
    Keywords: Fc epsilon receptor ; IgE immune complexes ; human cell lines and peripheral blood lymphocytes ; immunoperoxidase staining
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To identify Fc epsilon receptors on human cell lines and peripheral blood lymphocytes, we developed a new method which relies on the binding of constructed immune complexes to Fc epsilon receptor-positive cells. Cell suspensions from either cell lines or peripheral blood lymphocytes were incubated with complexes of human myeloma IgE and murine monoclonal anti-human IgE at various ratios prior to cytocentrifugation. The complexes bound to the cells were subsequently visualized by immunoperoxidase staining. The specificity of this assay to detect cell surface Fc epsilon receptors was shown by the ability of human myeloma IgE to block the binding of the IgE complexes, resulting in unstained cells, whereas IgM, IgG, and IgA were unable to block the binding of the complexes (stained cells). This method is reproducible, allows quantification of a single sample at different times, and provides a record of the results. It can also be adapted to identify any cell surface receptor for which the ligand is known.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of clinical immunology 1 (1981), S. 131-136 
    ISSN: 1573-2592
    Keywords: Immunodeficiency ; humoral ; hypogammaglobulinemia ; adenosine receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Since abnormal immune responsesin vivo may result from altered purine metabolism, we sought to delineate a potential role in humoral immunodeficiency for cells with specific adenosine membrane receptors. The purine nucleoside, adenosine, had been shown at low concentrationsin vitro to be capable of activating a subset of T lymphocytes to inhibit pokeweed mitogen (PWM)-driven IgG synthesis, while higher concentrations inhibited both IgG and IgM. Sixteen patients with common variable immunodeficiency and four patients with immunodeficiency with hyper-IgM were evaluated for the number of adenosine receptor cells in unfractionated mononuclear cells as well as T- and non-T-cell fractions. Theophylline-sensitive T cells, a subset of T lymphocytes which have been shown to be capable of suppressing immunoglobulin responsesin vitro, were also quantitated. Four of 16 patients with panhypogammaglobulinemia had clearly elevated levels of adenosine receptor cells. Two of these same individuals had low numbers of theophylline-sensitive cells, as did one patient with hyper-IgM. Only one person with variable immunodeficiency had increased theophylline-sensitive T cells. We found cases of clear-cut disparity between adenosine receptor and theophylline-sensitive cells. Furthermore, these markers segregated independently in both patients and normals. Finally, we assessed the effect of adenosine receptor cell removal onin vitro PWM-driven immunoglobulin synthesis. In none of the 20 patients tested did this cause a significant elevation of IgG or IgM production, nor did it have a consistent effect on cells from normals. Thus, while adenosine can be shown to profoundly alter immunoglobulin productionin vitro and some patients with humoral immunodeficiency have abnormal numbers of cells with adenosine receptors, we were unable to find evidence for a pathophysiologic role for these cells in immunoglobulin deficiency in humans.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of clinical immunology 8 (1988), S. 521-522 
    ISSN: 1573-2592
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1573-2592
    Keywords: Bullous pemphigoid ; basement membrane zone ; peripherial blood leukocytes ; autoimmunity ; immunoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Peripheral blood leukocytes (PBL) from patients with bullous pemphigoid stimulated by pokeweed mitogen (PWM) produce an anti-basement membrane-zone (BMZ) antibody. The antibody producedin vitro was biosynthetically radiolabeled, and its binding to the BMZ was histologically demonstrated by autoradiography. The IgG nature of the antibody was evidenced by the fact that it was absorbed by rabbit anti-human IgG antiserum immunoabsorbents. The peak antibody productionin vitro occurred after 6 days of culture. Antibody producedin vitro did not bind to the BMZ of human skin, but it did bind to the BMZ of the monkey esophagus. Normal human volunteers and control patients did not produce such an antibody. In other experiments the effects of adding serum containing anti-BMZ antibody to the synthesis of Ig by cells of patients and normal subjects were studied. Although serum from patients with bullous pemphigoid stimulated Ig production with either normal or BP-patient PBL, the difference was not statistically significant. The availability of the assay system allows for studying the immunoregulatory mechanism of autoantibody production in bullous pemphigoid.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Journal of clinical immunology 6 (1986), S. 306-309 
    ISSN: 1573-2592
    Keywords: hypogammaglobulinemia ; intravenous γ-globulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Immunoglobulin replacement therapy is required by patients with certain antibody deficiency syndromes and is finding increasing application in other immune disorders such as immune thrombocytopenia. We describe the long-term home administration of intravenous γ-globulin by seven patients themselves using a portable infusion pump. Over a period of as long as 2 years, this has proven to be effective, safe, and cost efficient and a good alternative to hospital or physicians' office-based infusions.
    Type of Medium: Electronic Resource
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