ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
–The total tRNA and some specific tRNAs from the 100,000g soluble fraction of rat brain were measured during development (postnatal ages 4–55 days). For determination of specific tRNAs we developed a method that measured their capacities to bind specific amino acids. Levels of total tRNA were decreased in the soluble fraction from the brains of 55-day-old rats in comparison to those for the 4-day-old rats. The aminoacylation capacities of tRNAs for phenylalanine, lysine, proline, valine, leucine, alanine and isoleucine were diminished in the 55-day-old rats in comparison to those for 4-day-old rats when expressed per unit wet weight of brain. When the 4- to 55-day changes in aminoacylation capacity of each specific tRNA was expressed relative to that of the total tRNA, tRNAPhe and tRNALysLys were diminished; tRNAPro, tRNAVel, tRNAGIY and tRNALeu showed no significant changes; and tRNAA1a and tRNAIle were increased. Incorporation of amino acids into a material insoluble in hot TCA (probably proteins) in a ribosome-free system occurred in the brain preparations. Out of ten different amino acids studied, arginine and tyrosine exhibited the highest values for this type of transfer.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1972.tb01282.x
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