ISSN:
0002-9106
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The response of the kidneys of 237 adult newts [Notophthalmus (Diemictylus) viridescens] to partial nephrectomy (15 to 30% of right kidney removed) and sub-total nephrectomy (70 to 90%) was studied histologically and autoradiographically to determine their regenerative potential. The response involved both hypertrophy and hyperplasia as indicated by increases in 3H-thymidine labelled nuclei and also 3H-leucine incorporation by the remaining cells of the kidney. Leucine incorporation increased within 24 hours and continued to increase until 5 days after partial nephrectomy (17% increase over control level) or 15 days after sub-total nephrectomy (36% increase). The number of thymidine labelled nuclei, however, did not increase for the first 5 days and then continued to increase up to 10 days after partial nephrectomy (to 3X control level) and 20 days after sub-total nephrectomy (to 5X control level).An accumulation of cells appeared on the cut surface of the kidney by 15 to 20 days after nephrectomy. It consisted of modified epithelial cells from the tubules and was characterized by marked basophilia. The number of 3H-thymidine labelled nuclei in the accumulation increased about 10 to 20 times over control levels at its peak on days 12 to 15; 3H-leucine incorporation doubled at its peak on days 10 to 15. Nevertheless, after day 20 the cell accumulation decreased in size due to cell resorption or sloughing or both; it had disappeared by day 50 with no new tissue resulting. The newt kidney does not appear to exhibit any regenerative potential and, therefore, it is similar to mammalian kidneys in this respect.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/aja.1001400304
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