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  • 1
    Electronic Resource
    Electronic Resource
    Tumour-cell-endothelial interactions: free radicals are mediators of melanoma-induced endothelial cell damage (1996)
    Springer
    Virchows Archiv 428 (1996), S. 99-106 
    Details
    ISSN: 1432-2307
    Keywords: Melanoma ; Endothelial cell damage ; Free radicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Damage to vascular endothelium may play an important role during metastasis. We used a three-dimensional model of tumour cell extravasation to test the hypothesis that certain types of tumour cells are able to induce vascular endothelial cell injury. Multicellular tumour spheroids (MCTS) of 14 human cancer cell lines and spheroids from two benign cell lines were transferred onto confluent monolayers of human endothelial cells (EC). MCTS from 4 of 7 melanoma cell lines induced damage of the endothelium which was closely associated with tumour cell attachment. Endothelial cell injury became evident morphologically by loss of cell membrane integrity and sensitivity to shear stress. Similar results were obtained with EC derived from human umbilical veins, umbilical arteries and saphenous veins. Addition of the oxygen radical scavenger catalase showed a dose- and time-dependent inhibition (up to 48 h) of EC damage in the case of the melanoma cell lines ST-ML-11, ST-ML-14 and SK-MEL-28. The scavenging enzyme superoxide dismutase proved to be protective (up to 12 h) in ST-ML-12 MCTS. In contrast, allopurinol, deferoxamine mesylate, ibuprofen, nor-dihydroguaretic acid, soybean trypsin inhibitor or aprotinin had no protective effect. None of the non-melanoma cancer cell lines or benign cells induced endothelial cell damage. Endothelial injury has been shown to enhance the process of metastasis. Our results suggest that free-radical-mediated endothelial cell damage may be one of the mechanisms contributing to the devastating metastatic potential of melanoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193937
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  • 2
    Electronic Resource
    Electronic Resource
    Unscheduled DNA synthesis and mitochondrial DNA synthetic rate following injury of the facial nerve (1997)
    Springer
    Acta neuropathologica 94 (1997), S. 557-566 
    Details
    ISSN: 1432-0533
    Keywords: Key words Motoneuron regeneration ; DNA repair ; Mitochondrial DNA synthesis ; Neuronal regeneration ; [3H]Thymidine ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Unscheduled DNA synthesis (UDS) of nuclear DNA and mitochondrial (mt) DNA synthetic rates were determined autoradiographically in different cell types of the rodent brain 14 days after unilateral facial nerve transection. In addition to an increased synthetic rate of mtDNA in facial motoneurons 12 h after axotomy, a significant increase of UDS, i.e., DNA repair, and mtDNA synthesis were found in the regenerating facial nucleus 4 days after axotomy. Specificity of the observed labeling was confirmed by injection of 3H2O instead of [3H]thymidine. Using electron microscopic autoradiography, it was further shown that cytoplasmic labeling of neurons was mainly due to incorporation of radioactive label into mitochondria, indicating their subsequent multiplication by division. The observation that Northern blot signals for O6-alkylguanine-DNA-alkyltransferase mRNA from homogenized facial nuclei of both the axotomized and normal side remained unchanged over 14 days after axotomy indicated that the observed DNA-repair activity was not caused by endogenously produced alkylating agents. The combined presence of transiently increased UDS, enhanced mtDNA synthesis and elevated protein synthetic rates of regenerating motoneurons (as shown in the literature) suggests that free radicals produced by mitochondria in injured nerve cells could cause unspecific DNA damage followed by immediate repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050750
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  • 3
    Electronic Resource
    Electronic Resource
    A cystic lesion within the dural sinuses: a rare cause of increased intracranial pressure (1997)
    Springer
    Neuroradiology 39 (1997), S. 132-135 
    Details
    ISSN: 1432-1920
    Keywords: Key words Hypertension ; intracranial ; Dural sinuses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We present a rare cause of intracranial hypertension in a 19-year-old woman. The torcular was obstructed by a cystic developmental lesion, thought preoperatively to be an epidermoid. The patient also had a second lesion of possibly developmental origin, a cerebral cavernous haemangioma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002340050380
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  • 4
    Electronic Resource
    Electronic Resource
    Microglial motility in the rat facial nucleus following peripheral axotomy (1999)
    Springer
    Journal of neurocytology 28 (1999), S. 439-453 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Microglial motility was studied in living mammalian brain tissue using infrared gradient contrast microscopy in combination with video contrast enhancement and time lapse video recording. The infrared gradient contrast allows the visualization of living cells up to a depth of 60 μm in brain slices, in regions where cell bodies remain largely uninjured by the tissue preparation and are visible in their natural environment. In contrast to other techniques, including confocal microscopy, this procedure does not require any staining or labeling of cell membranes and thus guarantees the investigation of tissue which has not been altered, apart from during preparation. Microglial cells are activated and increase in number in the facial nucleus following peripheral axotomy. Thus we established the preparation of longitudinal rat brainstem slices containing the axotomized facial nucleus as a source of activated microglial cells. During prolonged video time lapse recordings, two different types of microglial cell motility could be observed. Microglial cells which had accumulated at the surface of the slice remained stationary but showed activity of the cell soma, developing pseudopods of different shape and size which undulated and which were used for phagocytosis of cell debris. Microglial phagocytosis of bacteria could be documented for the first time in situ. In contrast, ameboid microglia which did not display pseudopods but showed migratory capacity, could be observed exclusively in the depth of the tissue. Some of these cells maintained a close contact to neurons and appeared to move along their dendrites, a finding that may be relevant to the role of microglia in “synaptic stripping”, the displacement of synapses following axotomy. This approach provides a valuable opportunity to investigate the interactions between activated microglial cells and the surrounding cellular and extracellular structures in the absence of staining or labeling, thus opening a wide field for the analysis of the cellular mechanisms involved in numerous pathologies of the CNS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007048903862
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  • 5
    Electronic Resource
    Electronic Resource
    Bildungswärmen von Fluor-Chlor-Kohlenstoff-Verbindungen (1955)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 278 (1955), S. 326-332 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Durch Umsatz von K mit CF3Cl, CF2Cl2, CFCl3, C2F4, C2F3Cl, C2F2Cl2 werden die Bildungswärmen dieser Verbindungen aus Graphit und den Halogenen gemessen. Die erhaltenen Werte werden mit gleichzeitig von KIRKBRIDE und DAVIDSON auf demselben Wege gewonnenen verglichen. Die Endresultate geben die Tabelle 3 und Abb. 2.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19552780514
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    Paper (German National Licenses)
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