ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The reaction between glucose and methylene blue, catalyzed by glucose oxidase (GOD)was analysed calorimetrically.The amount of heat produced under saturating methylene blue concentrations ( 〉 10-2 mol/1)was measured with glucose concentration and time as parameters (kinetic procedure) Kinetic constants (pseudo one substrate kinetics) were derived from the experimental data: KM(glucose)= 1.18 × 10-3 mol/l and Vmax = 0.085 J/mg GOD min (3.89 · 10-6 mol/mg GOD min)Comparison of caloric with optical measurements gave an enthalpy of reaction of 22.52 kJ/mol. Considering the observed substrate inhibition, glucose determinations are possible up to glucose concentrations of 0.1 mol/l.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370020114
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