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  • 1
    Electronic Resource
    Electronic Resource
    Structures and syntheses of two dihydropyrindines isolated from California petroleum (1972)
    s.l. : American Chemical Society
    The @journal of organic chemistry 37 (1972), S. 3834-3838 
    Details
    ISSN: 1520-6904
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jo00797a015
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  • 2
    Electronic Resource
    Electronic Resource
    Chlorfenprop-methyl: its hydrolysis in vivo and in vitro and a new principle for selective herbicidal action (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 17 (1977), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Chlorfenprop-mèthyl is hydrolysed completely as soon as it penetrates the leaf in sensitive as well as in resistant plants. The product of hydrolysis chlorfenprop presumably is the herbicidally active compound. The in vitro hydrolysing activity has been characterized.The behaviour of the herbicide in a system for the detection of rapid herbicidal activity (leaching system) and in three auxin-dependent systems leads to the conclusion, that a difference at the site of action is the basis for the observed herbicidal selectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3180.1977.tb00473.x
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  • 3
    Electronic Resource
    Electronic Resource
    BEKÄMPFUNG VON GALIUM APARINE MIT MECOPROP IN ABHÄNGIGKEIT VON VERSCHIEDENEN BODENARTEN (1972)
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 12 (1972), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Zusammenfassung. Die Bekämpfung von Galium aparine L. mit Mecoprop im Nachauflaufverfahren ist abhangig sowohl von der Pflanzengrösse ab auch vor allem vom Boden, in dem die Pflanzen wachsen:〈list xml:id="l1" style="custom"〉1 Kleine Pflanzen werden leichter vernichtet als grosse.2 Pflanzen, die auf einem Boden mit hohem organischen Anteil wachsen, sind gegen Mecoprop widerstandsfähiger als solche, die auf einem Boden mit niedrigem C-Gehalt wachsen.3Ein Teil der im Nachauflaufverfahren gespritzten Herbizidmenge gelangt auf den Boden und wirkt zusätzlich durch Aufnahme über die Wurzeln.4Diese zusätzlichc herbizide Wirkung ist auf den Bodenproben I und II mit hohem organischen Anteil geringer als auf dem Boden mit niedrigem C-Gehalt. Als Ursache der geringeren Wirksamkeit ist eine verstärkte Adsorption des Mecoprop in den Bodenproben I und II gegenüber Boden III verantwortlich.5Als Adsorbens kommt nur die organlsche Substanz der Bodenproben in Frage.Der zu erzielende Gesamtbekämpfungserfolg hängt neben der Aufnahme des Mecoprop über das Blatt auch von der zusätzlichen Wirkung des Herbizids über den Boden ab. Control of Galium aparine with mecoprop in relation to soil type
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3180.1972.tb01203.x
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  • 4
    Electronic Resource
    Electronic Resource
    A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) inChlorella sorokiniana (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1023-1044 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; Chlorella sorokiniana ; gene sequence ; glutamate dehydrogenase ; introns ; NADP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorella sorokiniana possesses ammonium-inducible, chloroplastic, NADP-specific glutamate dehydrogenase (NADP-GDH) homo- or heterohexamers composed of α- and/or β-subunits which were previously shown to derive from precursor protein(s) of identical size. From the present studies, data are consistent with these two subunits being encoded by a single nuclear gene. The NADP-GDH gene is greater than 7 kb in length due to the presence of at least 21 introns, an unusually large number for a eukaryotic microorganism. The exons, identified by comparison with sequences of NADP-GDH cDNA clones, include a region which is highly conserved among NADP-GDH genes. This region in theC. sorokiniana gene is 77% and 73% identical to the corresponding regions in theEscherichia coli andNeurospora crassa NADP-GDH genes, respectively. Seventeen independent NADP-GDH cDNA clones were analyzed by restriction mapping and partial sequencing, and no differences were detected among them. The longest cDNA was fused in frame withlacZ in a Bluescript vector and was expressed inE. coli as NADP-GDH antigen. During a 240 min induction period, under conditions in which both types of subunits were synthesized, only a single (2.2 kb) NADP-GDH mRNA band was detected on northern blots using cDNA probes from the highly conserved and 3′-untranslated regions. Collectively, these results are consistent with a single mRNA encoding a precursor-protein which is differentially processed to yield either an α- or β-subunit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037142
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  • 5
    Electronic Resource
    Electronic Resource
    Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-culturedChlorella sorokiniana cells (1994)
    Springer
    Journal of applied phycology 6 (1994), S. 211-223 
    Details
    ISSN: 1573-5176
    Keywords: Chlorella sorokiniana ; NADP-specific glutamate dehydrogenase gene/mRNAs ; promoter ; alternative splicing ; cell wall ; sporopollenin ; carotenoid-biosynthesis inhibition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorella sorokiniana may prove to be a useful organism in which to express genes/cDNAs from certain higher plants encoding enzymes catalyzing the synthesis of compounds of medical or industrial importance. This organism has a NADP-specific glutamate dehydrogenase (NADP-GDH) nuclear-gene encoding a precursor mRNA that appears to be alternatively spliced to yield mRNAs differing in size and encoding enzyme subunits with differing affinities for ammonium. The promoter of this gene may be useful to drive the expression of heterologous genes. The transcription initiation site of the NADP-GDH gene was identified. This site delineates the 3′-border of the promoter region, and 1285 bp upstream from this site have been sequenced. To identify the promoter 5′-border by deletion analysis, this region (coupled to a reporter gene in a plasmid vector) was to be introduced by electroporation intoC. sorokiniana protoplasts. Cells were treated with a mixture of hydrolytic enzymes; however, non-hydrolyzable component(s) in the cell wall inhibited protoplast formation. The cell wall was subsequently shown to contain acetolysis-resistant component(s) identified as sporopollenin-like in other algae. Since sporopollenin was reported earlier to be a polymer of carotenoids/carotenoid-esters, the cells were cultured in the presence of norflurazon, an inhibitor of carotenoid biosynthesis. Whereas synthesis of total carotenoids was inhibited by 75%, synthesis of the acetolysis-resistant component(s) in the cell wall was not inhibited. In addition to the above studies, a revised model for the regulation of expression of theC. sorokiniana NADP-GDH gene is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02186074
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  • 6
    Electronic Resource
    Electronic Resource
    Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-culturedChlorella sorokiniana cells (1994)
    Springer
    Journal of applied phycology 6 (1994), S. 437-438 
    Details
    ISSN: 1573-5176
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182164
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  • 7
    Electronic Resource
    Electronic Resource
    Alternative splicing of a precursor-mRNA encoded by the Chlorella sorokiniana NADP-specific glutamate dehydrogenase gene yields mRNAs for precursor proteins of isozyme subunits with different ammonium affinities (1998)
    Springer
    Plant molecular biology 37 (1998), S. 243-263 
    Details
    ISSN: 1573-5028
    Keywords: alternative splicing ; Chlorella sorokiniana ; chloroplastic ; glutamate dehydrogenase ; isozymes ; precursor mRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorella sorokiniana has seven ammonium-inducible, chloroplastic NADP-specific glutamate dehydrogenase (NADP-GDH) isozymes composed of varying ratios of α- and β-subunits. Southern blot and allele-specific PCR analyses indicate that the C. sorokiniana genome possesses a single 7178 bp nuclear NADP-GDH gene. cDNA cloning and sequencing, 5′-RACE-PCR analysis, and RNase protection analysis identified two NADP-GDH mRNAs that are identical with the exception of a 42 nt sequence located within the 5′-coding region of the longer mRNA. The 42 nt sequence, termed an auxon because it serves as an exon or intron, appears to undergo alternative splicing from the precursor mRNA by a process that is regulated by both nutritional and environmental signals. Depending upon whether the auxon is included or excluded in a mature mRNA, the gene can be considered to consist of 22 or 23 exons, respectively. The 2074 and 2116 nt mRNAs encode precursor proteins of 56350 and 57850 Da, respectively. The N-termini of the purified mature α- and β-subunits were sequenced, identifying full-length subunits of 53501 and 52342 Da, respectively. The sequences of the subunits are identical except for an 11 amino acid extension at the N-terminus of the α-subunit. The α-subunit has an additional α-helical domain at its N-terminus compared with the β-subunit. By correlating the abundances of the two mRNAs with the levels (and relative turnover rates) of the α- and β-subunit antigens during induction in Chlorella, the larger mRNA is proposed to encode the larger subunit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005921018137
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  • 8
    Electronic Resource
    Electronic Resource
    Nitrogen metabolism during synchronous growth of chlorella. II. Free-, peptide-, and protein-amino acid distributionThis paper represents part of the dissertation submitted by the senior author in partial fulfillment of the requirements of Doctor of Philosophy. (1970)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 75 (1970), S. 73-82 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The levels of free-, peptide-, and protein-amino acids were measured during the synchronous growth and division cycle of a thermophilic strain of Chlorella pyrenoidosa. Most of the protein amino acids exhibited little periodism (as % of total cellular-N); however, the free- and peptide-amino acids showed a variety of dramatic changes in level during the cell cycle.Fractionation of the acid-soluble peptides by Sephadex gel-filtration showed that an average of only 2.8% of the peptide amino acids were associated with peptides of high molecular weight (〉 5000), while approximately 75% of the peptide amino acids were components of low molecular weight peptides (〈 700). The low molecular weight peptides were predominately made up of relatively few amino acids (i.e., alanine, glutamate, lysine, glycine and arginine accounted for approximately 92% of the low molecular weight peptide amino acids).Several experiments revealed that nucleotide-peptides do not contribute significantly to the pool of acid-soluble peptides during the cell cycle of this organism.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040750109
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