ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The role of CD8 (Lyt-2) in the function of a long-term cytotoxic T-cell (CTL) clone (C196) was analysed. Previous studies had shown that C196 cells utilize the αβT-cell receptor and depend on Lyt-2 to recognize and lyse P815 mastocytoma target cells. Recognition is H-2Kd restricted, presumably involving a P815 specific antigenic structure. Here we analyse a number of variants selected from C196 that have reduced or virtually abolished expression of Lyt-2, alone or in combination with other deficiencies. We studied the ability of these variant cells to lyse either P185, a process requiring both specific antigen recognition and triggering of cytolytic function, or to lyse the anti-CD3 hybridoma 145-2C11, a process that requires the triggering of cytolytic function only. The results shows that in the case or a permanently activated CTL, effector cell such as C196, Lyt-2 is required for antigen recognition but is not essential for the triggering of cytotoxicity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1988.tb02417.x
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