ISSN:
1612-1112
Keywords:
Method development
;
System selection and optimization
;
Selectivity optimization
;
Method validation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary This paper provides an overview of method development in liquid chromatography. It is explained why every practicing chromatographer needs to understand the process of method development. Thus, this paper is directed towards chromatographers who develop new separation methods, as well as to those who apply published methods. The method-development process is first explained in broad terms, after which the different aspects of the process are described in more detail. First, attention is paid to the initial stages of method development, which consist of the accurate definition of the separation problem and the selection of the most suitable chromatographic method. Method-development expert systems are discussed with regards to their applicability in this area. Selectivity optimization is shown to rely on three main factors,i. e. the selection of the parameter space, the experimental design and the optimization criterion. Selectivity-optimization procedures are described very briefly. System optimization concerns those factors which do not affect the chemistry of the system (mobile and stationary phase), but are essentially determining the analysis time and the sensitivity. The method-validation stage can be divided into three main parts: qualitative method validation (i.e. peak integrity and identity), calibration and quantitative method validation. The requirements of calibration procedures are briefly reviewed and an inventory of method-validation procedures is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290483
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