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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Ribosomal DNA was surveyed in a world collection (43 lines) of Setaria italica for variation in repeat unit length and restriction enzyme site variability using a non-radioactive wheat rDNA probe. Only two lengths of repeat units of about 7.9 or 7.6 kb were observed. The fact that these two repeat unit lengths were not randomly dispersed throughout the world collection suggests the existence of three genetic groups. The central European lines and most western European accessions have only the 7.6 kb repeat unit and most Asiatic lines have a 7.9 kb repeat unit. Lines originating from the north or the south of Asia showed different numbers of Bam HI fragments. The fact that the difference between the Asiatic and European pool is not continuous (7.9 or 7.6 kb) excludes the hypothesis of domestication being based on the spread of an initial population over Eurasia.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 118 (1999), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The DNA of 37 lines of Setaria italica, representative of Eurasian diversity, were used for the detection of polymorphism using the random amplified polymorphic DNA (RAPD) method. Four 10-mer primers, differing from each other by one or two G-C inversions, were used. Each one gave specific RAPD products. Twenty-five bands were polymorphic and allowed the identification of 33 different genotypes. A factorial analysis of correspondence was performed on the presenc-absence data, through which three genetic groups could be identified. These genetic groups were closely related to the geographical origin of the different lines: one central European and two Asiatic groups (the first Asiatic lines originating in latitudes below 35°N and the second comprising the Asiatic lines originating in latitudes above 35° N). The lines originating from western Europe were very variable and were dispersed in the three genetic groups described previously.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The coding sequence of Δ9-stearoyl-(acyl carrier protein) desaturase from Ricinus communis was introduced into sunflower, under the control of seed-specific promoter and terminator sequences of the late embryogenesis abundant gene from sunflower, Hads10. Two independent primary transformants contained three and six copies of the T-DNA, as demonstrated by hybridization using nptII as a probe. The transgene proved genetically stable and was transmitted as a Mendelian trait. Transcript analysis of the heterologous Δ9-stearoyl-(acyl carrier protein) desaturase under control of the Hads10 promoter verified tissue-specific expression in the developing embryos and not in the leaves. Fatty acid composition of the seed oil was followed over five generations under greenhouse and open field conditions. Some of the transgenic lines produced oil with a significantly reduced stearic acid content compared with non-transformed plants under greenhouse and field conditions. However, additional studies need to be performed to assess whether or not physiologically stable lines can be developed from these transgenic lines.
    Type of Medium: Electronic Resource
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