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  • 1
    Electronic Resource
    Electronic Resource
    Induction, Specificity and Elimination of Asialo-GM 1+ Graft-Versus-Host Effector Cells of Donor Origin (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 34 (1991), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In previous studies we demonstrated that an induced asialo-GMI positive (ASGMI+) cell of donor origin that exerts natural killer cell-like activity (NK activity+) plays a crucial role in the development of graft-versus-host (GVH)-associated tissue damage and severe immunosuppression. This study examined whether the ASGMI+ (NK activity+) GVH effector cells were activated by non-specific signals or whether these cells were triggered by specific alloantigens and displayed antigenic specificity, C57B1/6 (B6) donor mice were treated with either B6xAF1 (B6AF1) lymphoid cells and anti-asialo GM 1 antibodies (anti-ASGM I) to induce and eliminate specifically activated B6–anti-B6AF1 ASGM1+ (NK activity’(cells or with polyinosinic: ploycytidylic acid (poly I:C), and anti-ASGMI to eliminate non-specifically activated ASGM1+ (NK activity +) cells. Donor spleen and lymph node cells depleted of the specific allo-induced ASGMI+ NK reactive cells showed near normal numbers of L3T4+ and Lyt-2+ cells and retained T- and B-cell functions as measured by mitogen responses (to PHA. Con A and LPS). mixed lymphocyte responses (MLR) (to B6Ar'i)and the generation of cytotoxic T cells (CTL) (to B6AF1 blasts). Anti-ASGMI treatment almost completely abrogated NK activity in all donor inocula. GVH reactions were induced by injecting treated donor cells into B6AF1. B6 × C3HejF1 (B6C3HF1) and B6 x SJLF1 (B6SJLF1) hybrids and monitored by splenomegaly, suppression of T-cell mitogen responses and the development of histopathological lesions in the thymus. liver and pancreas. Cells from donors depleted of non-specifically (poly 1:C) induced ASGMI+ cells induced severe histological lesions, marked immunosuppression and splenomegaly in all three F| hybrid combinations. When the donor cells were depleted of specifically induced (B6–anti-B6AF1) ASGMI+ cells and injected into the three F1 combinations they induced splenomegaly in all three but caused severe tissue injury and intense immunosuppression only in B6C3HF1 and B6SJLF1 mice and not in B6AF1, mice. Genetic analysis suggests that the H-2D (or a closely related) region of the H-2 complex plays an important role in the activation of the specific GVH effector cells. These results suggest that the cell(s) responsible for splenomegaly are different from the ones that cause severe GVH-associated tissue damage and immunosuppression although there may be cells and/or lymphokines common to both processes GVH-associated tissue injury appears to be due to the induction of an effector cell that expresses ASGMI and exerts both NK-cell activity and specific cytotoxicity but does not appear to be from the T-cell population that is responsible for specific MLR and CTL activities in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1991.tb01573.x
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  • 2
    Electronic Resource
    Electronic Resource
    ACQUIRED IMMUNE DEFICIENCY SYNDROME: SPECIFIC ASPECTS OF THE DISEASE IN HAITI (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 437 (1984), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1984.tb37143.x
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  • 3
    Electronic Resource
    Electronic Resource
    Common antigenicity between a human thymic epithelial cell product and a thymus-dependent serum factor (1982)
    Springer
    Cellular and molecular life sciences 38 (1982), S. 859-860 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific immunofluorescence of human thymic epithelial cytoplasm was obtained with antibodies to supernatant of thymic epithelial cultures, and with anti-prealbumin antibodies. These antibodies also reacted with normal serum but not with serum from Di George patients. The data indicates that thymic epithelium and a component of the prealbumin fraction of normal serum share a common antigen believed to be thymic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972317
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  • 4
    Electronic Resource
    Electronic Resource
    Stromal myofibroblasts in primary invasive and metastatic carcinomas (1981)
    Springer
    Virchows Archiv 391 (1981), S. 125-139 
    Details
    ISSN: 1432-2307
    Keywords: Myofibroblast ; Immunofluorescence ; Stroma ; Carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 23 primary invasive and 7 metastatic carcinomas was examined by light microscopy (LM), transmission electron microscopy (TEM) and immunofluorescence (IF), the latter employing an anti-actin antibody. The results were correlated with macroscopic features such as retraction and consistency. Stromal cells rich in actin, readily identified by IF in firm and retracted carcinomas, were rare or absent in neoplasms lacking these features. TEM established the myofibroblastic nature of these stromal cells. Alternate sections (LM, IF) of each neoplasm demonstrated that myofibroblasts were more numerous in “young” mesenchymal stroma than in densely sclerotic areas. The connective tissue adjacent to intraductal mammary carcinoma lacked myofibroblasts, suggesting that epithelial stromal invasion is required to evoke a myofibroblastic stromal response. Myofibroblasts which possess synthetic (type III collagen) and contractile properties may well contribute to the firm consistency and retraction which characterize many carcinomas. The induction of myofibroblasts might represent an important host stromal response directed toward containment of invasive and/or metastatic carcinoma. This response may be especially important in neoplasms with weak antigenicity and/or slow doubling times.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437591
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  • 5
    Electronic Resource
    Electronic Resource
    B cell lymphoproliferation in spontaneously diabetic BB Wistar rats (1982)
    Springer
    Diabetologia 23 (1982), S. 261-265 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes mellitus ; B cell lymphoma ; BB Wistar rat ; immunoblastic sarcoma ; B cell lymphoproliferation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ninety-six spontaneously diabetic BB Wistar rats were maintained for their natural life span and, at death, were autopsied together with 86 age- and sex-matched non-diabetic BB control rats. A 15% incidence of abdominal B cell lymphoproliferative lesions was documented in the diabetic rats compared with 1% incidence in the non-diabetic rats (p〈0.005). The B cell lymphoproliferative process included minute mesenteric and omental aggregates of plasma cells and small lymphocytes (one rat), atypical partially fibrotic lymphoproliferative mesenteric nodules (three rats), and malignant lymphoma with features of immunoblastic sarcoma (eight rats) or plasma cell lymphoma (two rats). Cytoplasmic immunoglobulin was demonstrated in two of the four lymphomas examined by the peroxidase-antiperoxidase technique, thus confirming their B cell derivation. The striking incidence of B cell lymphoproliferation in this diabetic population is additional evidence of altered immunity in this animal model of insulin-dependent diabetes mellitus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252852
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  • 6
    Electronic Resource
    Electronic Resource
    Pancreatic cellular infiltrates in autoimmune-prone New Zealand Black mice (1984)
    Springer
    Diabetologia 26 (1984), S. 310-313 
    Details
    ISSN: 1432-0428
    Keywords: New Zealand Black mice ; diabetes mellitus ; autoimmunity ; pancreatic cellular infiltrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Healthy New Zealand Black (NZB) mice of both sexes (age 19–31 weeks) were studied to determine the magnitude of pancreatic β cell injury related to mononuclear cell infiltration of islets. This investigation was undertaken following the description of spontaneous cellular immune reactions against islets in four strains of autoimmune-prone mice, including NZB mice [1]. Studies included complete autopsies with histological examination, determination of the pancreatic content of immunoreactive insulin, and the measurement of the plasma glucose concentration. Mononuclear cell infiltrates were identified in the lung, liver, kidney, salivary gland, mesentery, and pancreas. In the latter site, the infiltrates were situated in fibrous septae about ducts, ductules, and venules rather than islets. Only islets contiguous to infiltrates were involved, and then but focally. Insulitis, as manifest by the envelopment and permeation of islets by mononuclear cells, was not observed. In none was there a significant reduction of β cells or pancreatic insulin content, neither was hyperglycaemia manifest. This study reveals that, although NZB mice are subject to autoimmune phenomena and widespread mononuclear cell infiltrates, β cell injury and insulitis are not consistent features of this strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283656
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  • 7
    Electronic Resource
    Electronic Resource
    Class I and II major histocompatibility complex gene product expression by a rat insulinoma cell line in vitro following exposure to gamma interferon (1988)
    Springer
    Diabetologia 31 (1988), S. 675-680 
    Details
    ISSN: 1432-0428
    Keywords: Type 1 (insulin-dependent) diabetes mellitus ; rat insulinoma cell line ; gamma interferon ; immunocytochemistry ; major histocompatibility complex gene products ; pancreatic B cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A study of Class I and II major histocompatibility complex gene product expression by a rat insulinoma cell line (RINm5F) was performed using monoclonal antibodies and immunoperoxidase techniques. RINm5F cells were incubated with different concentrations of gamma interferon. RINm5F cells exhibit low levels of Class I molecules and are normally devoid of Class II gene products. Upon exposure to gamma interferon, RINm5F cells showed a dramatic increase in Class I expression. This expression was homogenous and could be detected on all cells after 18 h of incubation with as little as 1 unit/ml of interferon. In contrast, de novo Class II expression was not homogeneous and required 36 h of incubation with 10 units/ml of interferon. The number of RINm5F cells expressing Class II antigens was dose- and time-dependent. Interferon treatment did not affect the morphology of RINm5F cells as determined by ultrastructural analysis. Withdrawal of interferon from the culture medium for as long as 78 h diminished but did not abolish the expression of Class I and Class II molecules already induced. The ability of interferon to enhance expression of Class I gene products and induce de novo expression of Class II molecules on B-cell-derived RINm5F cells supports the hypothesis that aberrant expression of major histocompatibility complex gene products on pancreatic B cells may be an important factor in triggering the immune response in Type 1 (insulin dependent) diabetes mellitus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278751
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  • 8
    Electronic Resource
    Electronic Resource
    Increased vascular permeability in pancreas of diabetic rats: detection with high resolution protein A-gold cytochemistry (1992)
    Springer
    Diabetologia 35 (1992), S. 1118-1124 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes mellitus ; diabetic rat ; albumin ; protein A-gold ; vascular permeability ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The role of the pancreatic microcirculation in the pathogenesis of Type 1 (insulin-dependent) diabetes mellitus remains poorly understood. Herein, a method is described for the ultrastructural investigation of the integrity of the pancreatic microvasculature. The method consists of histochemical detection and isolation of the islets followed by albumin and protein A-gold immunocytochemistry, whereby the distribution of endogenous albumin is used as a marker of endothelial integrity. This technique, applied to the study of spontaneously diabetic rats, reveals a selective increase in permeability of islet capillaries and post-capillary venules at the onset of diabetes, while acinar capillaries and arterioles remain intact. At 50 days of age, before the onset of diabetes, the microvasculature of diabetes-prone rats shows no alterations in permeability to albumin. When used in conjunction with morphometric analyses, this methodological approach may be useful for further studies in pathologic or experimental conditions involving the pancreatic microvasculature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401364
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  • 9
    Electronic Resource
    Electronic Resource
    Niemann-Pick disease type C: nodular splenomegaly (1995)
    Springer
    Abdominal imaging 20 (1995), S. 157-160 
    Details
    ISSN: 1432-0509
    Keywords: Niemann-Pick type C disease ; Splenomegaly ; Splenic nodules ; Magnetic resonance imaging (MRI) ; Diagnostic imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Niemann-Pick type C disease (NPCD) is an autosomal recessive storage lipidosis due to a disorder of cholesterol esterification leading to the accumulation of sphingomyelin and cholesterol in the brain, liver, and spleen. The disease is usually diagnosed when neurological symptoms appear. We report an unusual presentation of NPCD in a young asymptomatic adult with isolated nodular splenomegaly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201528
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