ISSN:
1420-908X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Upregulation of ornithine decarboxylase (ODC) activity and polyamine levels is found in the kidney of MRL-lpr/lpr (lpr) mouse, an animal model of lupus. To understand the molecular genetics of ODC regulation inlpr mouse, we analyzed ODC mRNA and activity in the kidney oflpr and normal BALB/c and MRL-+/+ mice. Although ODC activity was significantly higher inlpr kidney, its mRNA level was lower compared to normal strains, as measured by Northern blot hybridization. Treatment oflpr mouse with difluoromethylornithine (DFMO) reduced ODC activity inlpr kidney to the level of normal strains. In contrast, ODC mRNA level increased 12-fold by DFMO treatment. These results suggest that post-transcriptional modification of ODC inlpr genetic background might be responsible for increased ODC activity and polyamines. The beneficial effect of DFMO on murine lupus suggests a pathogenic role for altered ODC regulation inlpr mouse.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01972767
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