ISSN:
1432-0703
Source:
Springer Online Journal Archives 1860-2000
Topics:
Energy, Environment Protection, Nuclear Power Engineering
,
Medicine
Notes:
Abstract The present study examines thein vitro incorporation of monovalent cations into rat erythroid cells as a model for evaluating the impairment of electrogenic transport by polychlorinated biphenyls (PCB) or polybrominated biphenyls (PBB) toxicity. Female Sprague-Dawley rats were fed 50 ppm polychlorinated biphenyls (PCB)Aroclor 1254®, Aroclor 1242® or 50 ppm polybrominated biphenyls (PBB) hexabromobiphenyl, or octabromobiphenyl supplemented in their normal diets for seven months. Isolated erythroid cells from each group were assessed for the amount of the incorporated cation86Rb. Because86Rb mimics K+ in membrane transport, it was used in these studies. Uptake of86Rb by erythrocytes in Aroclor 1254-treated group was depressed, compared to the control group in culture media depleted of K+. No changes occurred in cellular incorporation of86Rb in the control, Aroclor 1242, and two PBB treatment groups. In sodium-depleted medium, erythrocytes from only the Aroclor 1254-treated group showed minimal, though significant reduction, in86Rb incorporation. When erythroid cell suspensions from each treatment group were challenged with ouabain,86Rb uptake was depressed in all but the Aroclor 1254 group. This Aroclor 1254 group had cationic transport suppressed maximally by PCB and, therefore, was not responsive to further inhibition by ouabain. This study provides direct chemical evidence that PCB can cause damage to the cell sufficient enough to decrease active transport of monovalent cations. Polybrominated biphenyls differ from PCB Aroclor 1254 in that their effect on monovalent cationic transport is negligible. Furthermore, this investigation demonstrates that the effect of PCB on active transport is dependent on the particular isomeric mixture used, since Aroclor 1254 had an inhibiting effect but Aroclor 1242 had no effect on monovalent cationic transport.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01055813
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