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  • 1
    Electronic Resource
    Electronic Resource
    A new fluorescent method for the demonstration of macromolecular aldehydes (1975)
    Springer
    Histochemistry and cell biology 41 (1975), S. 353-359 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fluorochrome, p-hydrazinoacridine (HA), is used to stain selectively natural macromolecular aldehydes as occur in elastic tissue as well as periodic acid (oxidized) or hydrochloric acid (hydrolyzed) engendered polyaldehydes (e.g., respectively of glycogen, mucosubstances and deoxyribonucleic acid). The reaction is independent of SO2 required in Schiff-type reactions. The mechanism of the staining reaction is presumably via the formation of fluorescent hydrazones from aldehyde condensation with HA. Aldehyde blocking agents, i.e., thiosemicarbazide and sodium borohydride, block the staining reactions with HA. In view of the controversy surrounding the mechanism of Schiff-type reactions and the lack of an adequate explanation for the affinity of miscellaneous fluorochromes for elastic tissue, p-hydrazinoacridine appears to be an ideal reagent, combining a simple staining procedure, excellent histological results and a straightforward mechanism for its selectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00490077
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A new and unusual type of transport vesicle (1979)
    Springer
    Cell & tissue research 203 (1979), S. 499-502 
    Details
    ISSN: 1432-0878
    Keywords: Special vesicles ; Membrane transport ; Gastropoda ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The adventitial cells surrounding the spermatheca of the reproductive system of Sonorella santaritana (Mollusca: Gastropoda) appear to have an unusual system of vesicles. Electron micrographs of the membranes forming these vesicles show that they have multiple openings to the cell's exterior and that each opening has a “pore complex”. In addition, secondary vesicles appear to be generated by the primary vesicles. Evidence is presented suggesting that these vesicles represent a previously unreported membrane transport system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233278
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Localization of VIP-immunoreactive nerves in airways and pulmonary vessels of dogs, cats, and human subjects (1981)
    Springer
    Cell & tissue research 220 (1981), S. 231-238 
    Details
    ISSN: 1432-0878
    Keywords: Vasoactive intestinal peptide (VIP) ; Peptidergic nerves ; Pulmonary innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary VIP-containing neurons were localized in lungs from dogs, cats, and human subjects by means of the indirect immunofluorescence technique. Nerve fibers and terminals were observed in the smooth muscle layer and glands of airways, and within the walls of pulmonary and bronchial vessels, especially at the medial-adventitial junction. VIP-positive nerve cell bodies were identified in ganglia located in the walls of bronchi. These findings provide an anatomic basis for the possible modulation of airway and pulmonary vascular function by this neuropeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210505
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural analysis of the morphology and function of the spermatheca of the pulmonate snail, Sonorella santaritana (1980)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 163 (1980), S. 319-329 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ultrastructure of the spermatheca of the reproductive tract in the pulmonate snail, Sonorella santaritana, was investigated. This organ has a debris-filled lumen and an outer wall which can be divided into three distinct layers. The cell layer adjacent to the lumen is comprised of two cell types, tall columnar epithelial cells with microvilli and cells lacking microvilli. The next layer also has two cell types, muscle cells and apparent pigment cells. The most distant layer is an adventitia of large glycogen-containing cells. The lumen of the spermatheca contains a core of partially digested sperm and related materials.The luminal contents and the cellular morphology of this organ suggest that the spermathecal functions are both digestive and absorptive. It is proposed that excess sperm and related materials are transported to the spermatheca, digested, and the usable products are reabsorbed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051630306
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Tannic acid-glutaraldehyde fixation reveals calcium ionophore-induced changes in rabbit polymorphonuclear leukocyte membranes (1981)
    New York, NY : Wiley-Blackwell
    Journal of Supramolecular Structure and Cellular Biochemistry 16 (1981), S. 155-165 
    Details
    ISSN: 0275-3723
    Keywords: polymorphonuclear leukocyte ; rabbit ; tannic acid-glutaraldehyde ; ionophore ; A23187 ; degranulation ; membrane fine structure ; Chemistry ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: TAG fixation of normal and Ca2+ ionophore-treated rabbit polymorphonuclear leukocytes (PMN) has revealed membrane components not apparent with conventional glutaraldehyde fixation. These included a 30 Å external electron-dense coating on untreated cells. A somewhat thicker coat (40 Å) was observed in ionophore-treated, nondegranulating PMN. In ionophore-treated, degranulating PMN, a 65 Å cell membrane coat was observed. A similar coat was observed on the inner side of the membrane of some azurophil-type granules, but the electron density and thickness were not so pronounced. Cytoplasmic granules were often closely apposed and often protruded outward at the plasma membrane. Extracellular lamellae, sometimes stacked apposed to the plasma membrane, possibly represent remnants of intense granule extrusion. Sequential degranulation of the respective granules was not apparent.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jsscb.1981.380160206
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    Paper (German National Licenses)
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