ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
Presence of ice within cells usually is assumed to assure death, while larger ice formations are assumed to induce greater injury. Evidence is presented which questions these assumptions.MCSA and no. 440 parakeet tumors and C3H mouse skin were studied. Cell structure before freezing (control), while frozen, and after rewarming was correlated with survival on transplantation. Control and rewarmed tissue was fixed in Bouin's fluid and processed routinely. Microscopic sites and appearance of ice artifacts relative to frozen cells were preserved by the author's modified freeze-drying technique. Tissues were cooled to -75°C and -150°C at rates of from 1°C/min to 40°C/sec and rewarmed at 180°C/min. Tumor transplants were inoculated in and around the pectoralis major muscle of parakeets and followed as to appearance, size and microscopic structure. Using hair color as a marker, ventral to dorsal autografts of 8 mm full thickness circles of skin were made in 4-5 week old female mice.Data showed that (1) nucleus and cytoplasm were sites of ice formation in cooled cells, (2) little or no structural damage occurred in frozen-thawed tumors while skin often was altered noticeably, (3) % survival of tumors frozen slowly (1°C/min) was equal to that of controls, (4) 17% of frozen-thawed skin grafts survived, (5) freezethaw survival was greatest following the formation of the largest ice artifacts which were induced during slow freezing, (6) intracellular ice and structural alteration, therefore, need not be incompatible with survival.
Additional Material:
2 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091440302
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