ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A method is described for the quantification of tetrodotoxin (TTX) in crude extracts of puffer fish by capillary isotachophoresis. Conditions for isotachophoresis consisted of a leading electrolyte of 5 mM potassium acetate, pH 6.0, containing 0.2% Triton X-100 and 0.5 vol of dioxane, and a terminating electrolyte of 10 mM β-alanine adjusted to pH 4.5 with acetic acid. It was possible to quantify the TTX content of crude unfractionated puffer extracts, and obtain values similar to those obtained by mouse bioassays which are time consume.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1983.tb14870.x
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