ISSN:
1573-5001
Keywords:
EPSP synthase
;
fast freezing
;
narrowing linewidths
;
13P NMR
;
solid-state NMR
;
trehalose
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 13P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 ± 12 Hz and 125 ± 7 Hz respectively, by a variety of methods involving additives and freezing techniques.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008305118426
Permalink