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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 43 (1996), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Follicular dendritic cells (FDC) are unique non-lymphoid cells found only in lymph follicles. They play a part in the survival, proliferation and differentiation of B cells. To analyse the influence of FDC on B-lymphocyte proliferation, we isolatedthem from human tonsils on albumin gradients and treated them with mitomycin C to prevent the multiplication of lymphoid cells harboured in their cytoplasmic evaginations. FDC cultured for 12–16 h remained attached to the substrate;non-adherent cells were carefully eliminated by washing. Purified B cells cultured alone or with contaminant-cleared FDC were maintained for 2 days in the presence or absence of various stimulants, after which tritiated thymidine uptake by these cells was measured. In the absence of activators, FDC did not induce B-cell multiplication. B cells cultured in the presence of FDC exhibited increased 3H-TdR uptake when activated with anti-CD40 MoAb, anti-immunoglobulin MoAb or transferrin, but notwhen stimulated with Staphylococcus aureus strain Cowan I (SAC) at a given concentration. In the latter case, B-cell proliferation clearly decreased. In control cocultures where mitomycin-C-treated non-adherent cells were used instead of FDC inthe presence of the different stimulants, no increase in B-cell proliferation was observed. The results suggest that, inside the germinal centres, FDC modulation of B-cell proliferation depends on the activation state of the B cells and on the stimulantencountered.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 21 (1985), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Follicular dendritic cells (FDC) are located inside lymph follicles and are mainly characterized by their capacity to retain antigens. We investigated this aspect in mice lymph nodes by using bovine serum albumin (BSA) labelled with 5-nm colloidal gold particles and homologous anti-BSA antibodies bound to 20-nm gold particles. Gold-labelled BSA injected alone in non-immunized mice was only rarely found in FDC cytoplasmic interdigitations. Injected in the form of immune complexes, it was retained by FDC. Antigen-free anti-BSA antibodies injected under similar conditions as immune complexes were always found in draining lymph nodes in the same locations as BSA-anti-BSA immune complexes. F(ab′)2 from mouse immunoglobulins linked to colloidal gold particles were very rarely found between the FDC extensions, whereas it was intensely phagocytosed by macrophages. Our study permitted precise ultrastructural localization between FDC cytoplasmic extensions or inside macrophages and other cells of the lymph nodes, but it also pointed out that homologous antibodies linked to colloidal gold particles might be retained by FDC in the absence of antigens. These observations, carried out with colloidal gold, were checked by using 125I-labelled anti-BSA antibodies. Complement activation determinations of gold-labelled antibodies or immune complexes showed that antibodies or immune complexes fixed on colloidal gold particles do not activate the complement. This observation enabled us to conclude that Fc receptors play a significant part in the retention of gold-labelled antibodies or immune complexes by FDC of lymph nodes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biology of the Cell 73 (1991), S. 50 
    ISSN: 0248-4900
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 24 (1986), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using monoclonal anti-trinitrophenyl (TNP) antibodies complexed to TNP-myoglobin-coated gold particles, we analysed al the ultrastructural level the retention by follicular dendritic cells (FDC) of immune complexes containing various antibody isotypes. Gold-labelled immune complexes were injected subcutaneously or intravenously into naive mice and, after 24 h, germinal centres of draining lymph nodes or spleen were examined by electron microscopy. FDC generally retained complexes containing IgG2a and IgG2b better than those formed with IgGl or IgG3. IgM was rarely retained. FDC isolated from lymph nodes or spleens were incubated in vitro with gold-labelled complexes in a serum-free medium. IgG2a and IgG2b complexes were also retained in vitro in large quantities by FDC; IgGl and IgG3 complexes were retained in smaller quantities or in highly variable quantities compared with IgG2; IgM complexes were rarely seen on FDC. There was no difference between FDC isolated from lymph nodes or from spleen with respect to the Ig isotypes required for Fe-mediated retention of immune complexes.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 1505-1506 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural autoradiographic studies of mouse thymic blast cells after H3 Tdr injection show that their fine nuclear structure is related to their position in the cell cycle. The variations in the composition of the subcapsular blast cell population during radiation-induced leukemogenesis indicate kinetic changes in thymic lymphopoiesis, which are probably due to the oncogenic process.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cancer immunology immunotherapy 2 (1977), S. 179-187 
    ISSN: 1432-0851
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We treated thirty seven patients suffering from a clinical stage I malignant melanoma by immunosurgery and observed them for a three-year period ending December 1975. Prior to surgery the tumor was submitted to the application of DNCB. Patients were divided between those who had or had not been subjected to biopsy before starting treatment. Accordingly, 23 patients treated by immunosurgery as the first treatment, were called the “primitive treatment” group and 14 patients subjected to a previous biopsy, the “secondary treatment” group. In the “primitive treatment” group, all 23 patients survived without any sign of recurrence. Relapse was observed in 4 out of 14 patients of the biopsy group. Extensive histological investigations have been made by light and electron microscopes. The tumors were infiltrated and disrupted by a large number of mononucleated cells. The majority of infiltrating cells were small lymphocytes. True lymphatic follicles have been observed in the skin around the melanoma.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A technique for demonstration of glycosaminoglycans and other biological polyanions has been applied on floating thin sections for electron microscopy. The method is based on the formation of insoluble polyanions-quaternary ammonium salt complexes and the subsequent formation of electron dense ion association complexes following a ferric thiocyanate treatment. Mast cell granules and eosinophilic granule crystalloids were selectively stained. Nucleic acid contrast was selective to some extends.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 66 (1980), S. 49-58 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 μg/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 99 (1969), S. 235-251 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Chez la souris C57 Bl, 2% des sections de noyaux de plasmocytes ganglionnaires contiennent un corps nucléaire, rarement plus. Chez les animaux immunisés par une injection d'hématies hétérologues, le pourcentage de noyaux plasmocytaires contenant un ou plusieurs corps nucléaires augmente jusqu'à 14% du 4ème au 9ème jour qui suivent l'administration de l'antigène. Cette augmentation est parallèle à l'élévation du taux des anticorps sériques. De plus, au même moment, le nombre des noyaux contenant plusieurs corps nucléaires augmente proportionnellement plus que celui des sections n'en contenant qu'un. Les corps nucléaires ont un diamètre moyen de 1,5 μ. Ils sont principalement formés d'un matériel finement granulaire ou filamenteux dont les éléments constitutifs ont un diamètre de 60 à 70 Å et de granules denses de 250 à 280 Å de diamètre. On en distingue plusieurs types selon la proportion relative et la disposition de ces constituants. Certains contiennent également des formations sphériques plus volumineuses dont le diamètre varie de 1000 à 1400 Å. L'étude ultrastructurale de ces corps après incubation des coupes en présence soit de RNAase soit de DNAase, révèle que les fins granules sont constitués de RNA, tandis que les grains denses représentent du DNA. Les digestions enzymatiques font apparaître une disposition en spirale de ces derniers éléments. Quant aux plus volumineuses formations sphériques, elles subsistent après les deux digestions et apparaissent comme étant en fait des tubes denses à double membrane. L'origine des corps nucléaires est discutée ainsi que la relation entre leur présence et la synthèse des protéines.
    Notes: Summary In the C57 Bl mouse, 2% of the sections of plasma cells nuclei from lymphnodes contain one nuclear body (N.B.). The occurrence of two or several bodies per nuclear section is rare. The percentage of plasma cell nuclear sections with one or more nuclear bodies rises up to 14% from the 4th to the 9th day after the injection of heterologous erythrocytes. This increase is parallel to that of blood antibodies. The number of nuclei with several N.B. increases more than that of nuclei with only one. These nuclear bodies have a mean diameter of 1.5 μ. They are made up of a finely granular or filamentous material (60 to 70 Å in diameter) and of larger dense granules (250 to 280 Å). According to the relative distribution of these constituents, several types of N.B. can be identified. Some of these contain also larger spherical granules with a diameter of about 1000 to 1400 Å. Sections have been incubated with RNAase or DNAase. The fine granules disappear with the former while the dense grains are attacked by the latter enzyme. These digestions show a spiral distribution of the dense grains. The large spherical structures are not modified by these treatments but they are then found to be dense tubes with double membranes. The origin of the nuclear bodies is discussed as well as their relationship to protein synthesis.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 83 (1967), S. 249-264 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Après une injection unique d'antigène à des souris C 57 B1, la poussée plasmocytaire dans la médullaire ganglionnaire se développe parallèlement à la montée du taux des anticorps circulants du sérum. Ces plasmocytes semblent provenir principalement de la transformation de grandes cellules pyroninophiles. Celles-ci, présentes préalablement dans le cortex, pourraient provenir de la dislocation précoce des centres germinatifs. Elles ne peuvent se transformer en plasmocytes que dans la médullaire. Ces modifications ne pourraient se faire que lorsque la cellule pyroninophile est intégrée au sein d'un ≪ilôt plasmocytaire≫, c'est-à-dire d'un ilôt dans lequel cette cellule pyroninophile entre étroitement en contact avec un macrophage. A côté de cette origine principale, des plasmocytes peuvent provenir directement de la transformation du lymphoblaste. Enfin, nous avons observé que certaines cellules réticulées se modifient après l'injection de l'antigène. Ces transformations aboutissent à la formation de cellules ayant plusieurs caractères du plasmocyte, sans cependant les présenter tous, d'oú le qualificatif de ≪plasmocytoïde≫ que nous leur avons donné.
    Notes: Summary After a single injection of sheep erythrocytes to C 57 Bl mice, the increase in the number of plasma cells in the medullary cords of lymph nodes parallels the humoral antibody titers. The appearance of these plasma cells has been followed ultrastructurally. Most of them arise by modification of the large pyroninophilic cells. These appear at first in the cortex, and could be formed by an early dislocation of the germinal centers. However, the transformation into plasma cells finds place only in the medulla. These changes seem to occur only when pyroninophilic cells are included in a “rosette” (“ilôt plasmocytaire”), where they surround a macrophage and develop close connections with it. Other plasma cells may originate directly from lymphoblasts. After the injection of the antigen, some reticular cells are modified into “plasmacytoid cells” sharing some, but not all, of the morphological characteristics of plasma cells.
    Type of Medium: Electronic Resource
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