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  • 1
    Electronic Resource
    Electronic Resource
    NADP+ glyceraldehyde-3-phosphate dehydrogenase in soybeans [Glycine max (L.) Merr.]: genetics and developmental expression (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 851-856 
    Details
    ISSN: 1432-2242
    Keywords: (NADP+) glyceraldehyde-3-phosphate dehydrogenase ; Zymogram ; Allelic shift ; Inheri-tance ; Developmental expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chloroplastic (NADP+) glyceraldehyde-3-phosphate dehydrogenase (E.C. 1.2.1.9) catalyzes the second reaction in photosynthesis after the fixation of carbon by RuBisCO. Chloroplast-bound (NADP+) G3PDH was resolved in soybean by starch gel electrophoresis using l-histidine-citrate buffer (pH 5.7). Histochemical staining revealed zymogram patterns indicative of a tetramer. A survey of soybean genotypes revealed differences in zymogram patterns between the principal cytoplasmic sources of the northern and southern US germplasms. In the soybean pedigree, an allelic frequency shift toward a five-banded pattern was observed. G3PDH polymorphism was due to allele associated with gene expression at the slow locus. No linkage was found between the slow locus of (NADP+) G3PDH and AC02, AC03, AC04, ACP, DIA1, IDH1, IDH2, PGM1, and PGM3. Developmental expression in the above-ground tissues was identical, whereas roots as a rule did not express (NADP+) G3PDH activity. The importance of chloroplast-bound (NADP+) G3PDH in photo-synthesis and its interesting mode of inheritance warrants further exploration of this enzyme in soybean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225028
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Resistance to soybean cyst nematode and molecular polymorphism in various sources of Peking soybean (1994)
    Springer
    Euphytica 75 (1994), S. 63-70 
    Details
    ISSN: 1573-5060
    Keywords: cyst nematode ; Heterodera glycines ; diversity ; resistance ; RFLP ; RAPD ; soybean ; Glycine max
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cultivar Peking has been extensively used as a source of resistance to Race 3 and Race 5 of soybean cyst nematode, Heterodera glycines I., and Peking genes for resistance are present in a wide range of resistant soybean cultivars. Peking is also used as a host differential in the soybean cyst nematode race classification system. Thirteen Peking lines maintained in the USDA Soybean Germplasm Collection and in several breeding programs were surveyed using RFLP and RAPD markers for genetic characterization. Based on the molecular diversity combined with reaction to soybean cyst nematode, Peking genotypes from a common original source were identified. Peking lines PI 297543 (introduction from Hungary), and PI 438496A, PI 438496B and PI 438496C (introductions from Russia) represented unrelated germplasms. Identified molecular polymorphism can be used to validate the genetic purity of Peking lines used as host differentials for soybean cyst nematode classification system as well as utilization of an individual germplasm line in genetic-breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024532
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    Paper (German National Licenses)
    Fulltext
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